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AB283980

Human ALKBH3 ELISA Kit

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Human ALKBH3 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human ALKBH3 in Human in Cell/Tissue Extracts samples.

View Alternative Names

ABH3, DEPC1, ALKBH3, Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3, Alkylated DNA repair protein alkB homolog 3, DEPC-1, Prostate cancer antigen 1, hABH3

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Sandwich ELISA - Human ALKBH3 ELISA Kit (AB283980)
  • sELISA

Supplier Data

Sandwich ELISA - Human ALKBH3 ELISA Kit (AB283980)

Example of Human ALKBH3 standard curve.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate, Tissue

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.17 ng/mL

Range

0.625 - 5 ng/mL

Assay time

5h

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

The Human ALKBH3 ELISA Kit (ab283980) is designed for detection of ALKBH3 in human cell lysate and tissue samples

This assay employs a quantitative sandwich enzyme immunoassay technique that measures human ALKBH3 in approximately 5 hours. A polyclonal antibody specific for human ALKBH3 has been pre-coated onto a 96-well microplate with removable strips. ALKBH3 in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for human ALKBH3, which is recognized by a streptavidin-peroxidase (SP) conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The colour development is stopped and the intensity of the colour is measured.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 60, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.6" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 60, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.8" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab283980-1x96Tests", "assetComponentDetails": [ { "size":"2 x 30 mL", "name":"20X Wash Buffer Concentrate", "number":"AB283980-CMP06", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Solution", "number":"AB283980-CMP09", "productcode":"" }, { "size":"1 x 7 mL", "name":"Chromogen Substrate", "number":"AB283980-CMP04", "productcode":"" }, { "size":"1 x 120 µL", "name":"50X Biotinylated Human ALKBH3 Antibody", "number":"AB283980-CMP07", "productcode":"" }, { "size":"1 x 80 µL", "name":"100X Streptavidin-Peroxidase Conjugate", "number":"AB283980-CMP10", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Diluent M Concentrate", "number":"AB283980-CMP01", "productcode":"" }, { "size":"1 x 1 Vial", "name":"ALKBH3 Standard", "number":"AB283980-CMP03", "productcode":"" }, { "size":"3 x 1 Unit", "name":"Sealing Tapes", "number":"AB283980-CMP08", "productcode":"" }, { "size":"1 x 2 mL", "name":"1X Standard Diluent", "number":"AB283980-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human ALKBH3 Microplate", "number":"AB283980-CMP05", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Product protocols

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Target data

Dioxygenase that mediates demethylation of DNA and RNA containing 1-methyladenosine (m1A) (PubMed : 12486230, PubMed : 12594517, PubMed : 16174769, PubMed : 26863196, PubMed : 26863410). Repairs alkylated DNA containing 1-methyladenosine (m1A) and 3-methylcytosine (m3C) by oxidative demethylation (PubMed : 12486230, PubMed : 12594517, PubMed : 16174769, PubMed : 25944111). Has a strong preference for single-stranded DNA (PubMed : 12486230, PubMed : 12594517, PubMed : 16174769, PubMed : 20714506). Able to process alkylated m3C within double-stranded regions via its interaction with ASCC3, which promotes DNA unwinding to generate single-stranded substrate needed for ALKBH3 (PubMed : 22055184). Can repair exocyclic 3,N4-ethenocytosine adducs in single-stranded DNA (PubMed : 25797601). Also acts on RNA (PubMed : 12594517, PubMed : 16174769, PubMed : 16858410, PubMed : 26863196, PubMed : 26863410). Demethylates N(1)-methyladenosine (m1A) RNA, an epigenetic internal modification of messenger RNAs (mRNAs) highly enriched within 5'-untranslated regions (UTRs) and in the vicinity of start codons (PubMed : 26863196, PubMed : 26863410). Requires molecular oxygen, alpha-ketoglutarate and iron (PubMed : 16858410, PubMed : 22055184).
See full target information ALKBH3
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