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AB108852

Human Alpha 1 Acid Glycoprotein / AGP ELISA Kit (AGP)

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(4 Publications)

Human Alpha 1 Acid Glycoprotein / AGP ELISA Kit (AGP) is a Sandwich ELISA for the measurement of Human Alpha 1 Acid Glycoprotein / AGP (AGP) in Human in Biofluids samples.

View Alternative Names

AGP1, ORM1, Alpha-1-acid glycoprotein 1, AGP 1, Orosomucoid-1, OMD 1

1 Images
Sandwich ELISA - Human Alpha 1 Acid Glycoprotein / AGP ELISA Kit (AGP) (AB108852)
  • sELISA

Supplier Data

Sandwich ELISA - Human Alpha 1 Acid Glycoprotein / AGP ELISA Kit (AGP) (AB108852)

Representative Standard Curve using ab108852.

Key facts

Detection method

Colorimetric

Sample types

Cerebral Spinal Fluid, Saliva, Urine, Plasma, Milk, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 2 ng/mL

Range

3.125 - 200 ng/mL

Assay time

4h

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's Alpha 1 Acid Glycoprotein / AGP Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of AGP levels in cell culture supernatant, saliva, milk, and urine.

An Alpha 1 Acid Glycoprotein / AGP specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an Alpha 1 Acid Glycoprotein / AGP specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Alpha 1 Acid Glycoprotein / AGP captured in plate.

The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9.7" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha 1 Acid Glycoprotein also known as AGP or Alpha 1 Glycoprotein is a plasma protein with a molecular weight of approximately 41 to 43 kDa. This protein circulates primarily in the bloodstream and is produced mainly by the liver. AGP is known for its highly glycosylated structure carrying several branched oligosaccharide chains. It exhibits a significant capacity to bind various endogenous and exogenous lipophilic substances influencing their transport and distribution in the body.
Biological function summary

AGP plays a role in modulating the immune response and maintaining homeostasis. As an acute-phase protein its levels rise significantly during inflammation infection and trauma where it acts to modulate the immune system. AGP does not exist as part of a larger protein complex but interacts with plasma lipoproteins and other glycoproteins aiding in its function of maintaining vascular integrity during acute-phase reactions.

Pathways

Alpha 1 Glycoprotein serves as an important component in the inflammatory response and drug-binding pathways. In the inflammatory pathway AGP interacts with cytokines and acute-phase reactants adjusting the body’s inflammatory processes. It is also involved in the binding and distribution of drugs interacting with albumin and other transport proteins to influence the pharmacokinetics of many medications.

AGP levels change in response to conditions like rheumatoid arthritis and liver diseases. In rheumatoid arthritis its increased levels correlate with disease activity and inflammation. In liver diseases the production and plasma concentration of AGP can reflect hepatic synthesis capability. AGP's interaction with cytokines in these conditions often links it to elevated levels of other acute-phase proteins like C-reactive protein reflecting its participation in the pathophysiological processes of inflammation.
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Product protocols

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Target data

Functions as a transport protein in the blood stream. Binds various ligands in the interior of its beta-barrel domain. Also binds synthetic drugs and influences their distribution and availability in the body. Appears to function in modulating the activity of the immune system during the acute-phase reaction.
See full target information ORM1
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 10:3844 PubMed32123229

2020

Serum proteomics of active tuberculosis patients and contacts reveals unique processes activated during Mycobacterium tuberculosis infection.

Applications

Unspecified application

Species

Unspecified reactive species

Jesús Mateos,Olivia Estévez,África González-Fernández,Luis Anibarro,Ángeles Pallarés,Rajko Reljic,Tufária Mussá,Cremildo Gomes-Maueia,Artur Nguilichane,José M Gallardo,Isabel Medina,Mónica Carrera

International journal of chronic obstructive pulmo 15:311-321 PubMed32103930

2020

Circulating Biomarkers of Handgrip Strength and Lung Function in Chronic Obstructive Pulmonary Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Rizwan Qaisar,Asima Karim,Tahir Muhammad

American journal of physiology. Gastrointestinal and liver physiology 318:G305-G312 PubMed31736338

2019

Urine α-fetoprotein and orosomucoid 1 as biomarkers of hepatitis B virus-associated hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Zhu Zhan,Yalan Guan,Kenley Mew,Weiqiong Zeng,Mingli Peng,Peng Hu,Yixuan Yang,Yi Lu,Hong Ren

PloS one 8:e70341 PubMed23940561

2013

Polymorphism of ORM1 is associated with the pharmacokinetics of telmisartan.

Applications

Unspecified application

Species

Unspecified reactive species

Wang-Qing Chen,Yan Shu,Qing Li,Lin-Yong Xu,Mary W Roederer,Lan Fan,Lan-Xiang Wu,Fa-Zhong He,Jian-Quan Luo,Zhi-Rong Tan,Yi-Jing He,Hong-Hao Zhou,Xiang Chen,Wei Zhang
View all publications
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