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AB108854

Human Alpha 1 acid Glycoprotein / AGP ELISA Kit (AGP)

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(8 Publications)

Human Alpha 1 acid Glycoprotein / AGP ELISA Kit (AGP) is a competitive ELISA designed to quantify Human Alpha 1 acid Glycoprotein (AGP) with a sensitivity of 94 ng/mL

- Colorimetric competitive ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 0.25 - 2 µg/ml

View Alternative Names

AGP1, ORM1, Alpha-1-acid glycoprotein 1, AGP 1, Orosomucoid-1, OMD 1

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Competitive ELISA - Human Alpha 1 acid Glycoprotein / AGP ELISA Kit (AGP) (AB108854)
  • cELISA

Supplier Data

Competitive ELISA - Human Alpha 1 acid Glycoprotein / AGP ELISA Kit (AGP) (AB108854)

Representative Standard Curve using ab108854.

Competitive ELISA - Human Alpha 1 acid Glycoprotein / AGP ELISA Kit (AGP) (AB108854)
  • cELISA

Lab

Competitive ELISA - Human Alpha 1 acid Glycoprotein / AGP ELISA Kit (AGP) (AB108854)

Alpha1 acid Glycoprotein / AGP measured in various biological fluids showing quantity (ng) per mL of sample tested

Key facts

Detection method

Colorimetric

Sample types

Plasma, Serum

Reacts with

Human

Assay type

Competitive

Sensitivity

= 94 ng/mL

Range

0.25 - 2 µg/mL

Assay time

3h

Assay Platform

Microplate

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Reactivity data

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Product details

Abcam's Alpha 1 acid Glycoprotein / AGP Human in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitive measurement of Alpha 1 acid Glycoprotein / AGP in plasma and serum.

An Alpha 1 acid Glycoprotein / AGP specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Alpha 1 acid Glycoprotein / AGP is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Alpha 1 acid Glycoprotein / AGP captured in plate.

The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "11.1" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.3" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha 1 Acid Glycoprotein also known as AGP or Alpha 1 Glycoprotein is a plasma protein with a molecular weight of approximately 41 to 43 kDa. This protein circulates primarily in the bloodstream and is produced mainly by the liver. AGP is known for its highly glycosylated structure carrying several branched oligosaccharide chains. It exhibits a significant capacity to bind various endogenous and exogenous lipophilic substances influencing their transport and distribution in the body.
Biological function summary

AGP plays a role in modulating the immune response and maintaining homeostasis. As an acute-phase protein its levels rise significantly during inflammation infection and trauma where it acts to modulate the immune system. AGP does not exist as part of a larger protein complex but interacts with plasma lipoproteins and other glycoproteins aiding in its function of maintaining vascular integrity during acute-phase reactions.

Pathways

Alpha 1 Glycoprotein serves as an important component in the inflammatory response and drug-binding pathways. In the inflammatory pathway AGP interacts with cytokines and acute-phase reactants adjusting the body’s inflammatory processes. It is also involved in the binding and distribution of drugs interacting with albumin and other transport proteins to influence the pharmacokinetics of many medications.

AGP levels change in response to conditions like rheumatoid arthritis and liver diseases. In rheumatoid arthritis its increased levels correlate with disease activity and inflammation. In liver diseases the production and plasma concentration of AGP can reflect hepatic synthesis capability. AGP's interaction with cytokines in these conditions often links it to elevated levels of other acute-phase proteins like C-reactive protein reflecting its participation in the pathophysiological processes of inflammation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Functions as a transport protein in the blood stream. Binds various ligands in the interior of its beta-barrel domain. Also binds synthetic drugs and influences their distribution and availability in the body. Appears to function in modulating the activity of the immune system during the acute-phase reaction.
See full target information ORM1
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

BMC sports science, medicine & rehabilitation 14:100 PubMed35658935

2022

Orosomucoid: a promising biomarker for the assessment of exercise-induced fatigue triggered by basic combat training.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Ruan,Ke-Fa Xiang,Hui-Min Zhang,Zhen Qin,Yang Sun,Jing-Jing Wan,Wei Gu,Xia Liu

EJHaem 2:349-356 PubMed35844700

2021

Age-specific differences in the magnitude of malaria-related anemia during low and high malaria seasons in rural Zambian children.

Applications

Unspecified application

Species

Unspecified reactive species

Clement O Acheampong,Maxwell A Barffour,Kerry J Schulze,Justin Chileshe,Ng'andwe Kalungwana,Ward Siamusantu,Keith P West,Amanda C Palmer

The Journal of pediatrics 213:74-81.e1 PubMed31402145

2019

Relative Contributions of Malaria, Inflammation, and Deficiencies of Iron and Vitamin A to the Burden of Anemia during Low and High Malaria Seasons in Rural Zambian Children.

Applications

Unspecified application

Species

Unspecified reactive species

Maxwell A Barffour,Kerry J Schulze,Ng'andwe Kalungwana,William J Moss,Keith P West,Justin Chileshe,Ward Siamusantu,Amanda C Palmer

The Journal of nutrition 148:1160-1166 PubMed29924320

2018

Nutritional Status Measures Are Correlated with Pupillary Responsiveness in Zambian Children.

Applications

Unspecified application

Species

Unspecified reactive species

Katherine Healy,Amanda C Palmer,Maxwell A Barffour,Kerry J Schulze,Ward Siamusantu,Justin Chileshe,Keith P West,Alain B Labrique

The American journal of tropical medicine and hygiene 98:334-343 PubMed29165226

2018

Comparability of Inflammation-Adjusted Vitamin A Deficiency Estimates and Variance in Retinol Explained by C-Reactive Protein and α-Acid Glycoprotein during Low and High Malaria Transmission Seasons in Rural Zambian Children.

Applications

Unspecified application

Species

Unspecified reactive species

Maxwell A Barffour,Kerry J Schulze,Christian L Coles,Justin Chileshe,Ng'andwe Kalungwana,Margia Arguello,Ward Siamusantu,William J Moss,Keith P West,Amanda C Palmer

Tropical medicine & international health : TM & IH 23:53-62 PubMed29121448

2017

Malaria exacerbates inflammation-associated elevation in ferritin and soluble transferrin receptor with only modest effects on iron deficiency and iron deficiency anaemia among rural Zambian children.

Applications

Unspecified application

Species

Unspecified reactive species

Maxwell A Barffour,Kerry J Schulze,Christian L Coles,Justin Chileshe,Ng'andwe Kalungwana,Ward Siamusantu,Margia Arguello,William J Moss,Keith P West,Amanda C Palmer

The Journal of nutrition 147:1531-1536 PubMed28701387

2017

High Iron Stores in the Low Malaria Season Increase Malaria Risk in the High Transmission Season in a Prospective Cohort of Rural Zambian Children.

Applications

Unspecified application

Species

Unspecified reactive species

Maxwell A Barffour,Kerry J Schulze,Christian L Coles,Justin Chileshe,Ng'andwe Kalungwana,Margia Arguello,Ward Siamusantu,William J Moss,Keith P West,Amanda C Palmer

The American journal of clinical nutrition 104:181-90 PubMed27169838

2016

Provitamin A-biofortified maize increases serum β-carotene, but not retinol, in marginally nourished children: a cluster-randomized trial in rural Zambia.

Applications

cELISA

Species

Human

Amanda C Palmer,Ward Siamusantu,Justin Chileshe,Kerry J Schulze,Maxwell Barffour,Neal E Craft,Ngosa Molobeka,Ng'andwe Kalungwana,Margia A Arguello,Maithilee Mitra,Bess Caswell,Rolf Dw Klemm,Keith P West
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Associated Products

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