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AB243675

Human alpha 1 Acid Glycoprotein ELISA Kit

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(2 Publications)

Human alpha 1 Acid Glycoprotein ELISA Kit is a single-wash 90-min Simplestep used to quantify Human alpha 1 Acid Glycoprotein with a sensitivity of 68.5 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

AGP1, ORM1, Alpha-1-acid glycoprotein 1, AGP 1, Orosomucoid-1, OMD 1

6 Images
ELISA - Human alpha 1 Acid Glycoprotein ELISA Kit (AB243675)
  • ELISA

Supplier Data

ELISA - Human alpha 1 Acid Glycoprotein ELISA Kit (AB243675)
Sandwich ELISA - Human alpha 1 Acid Glycoprotein ELISA Kit (AB243675)
  • sELISA

Supplier Data

Sandwich ELISA - Human alpha 1 Acid Glycoprotein ELISA Kit (AB243675)

Interpolated concentrations of native alpha 1 Acid Glycoprotein in human urine, milk, saliva and HepG2 cell culture supernatant samples.

The concentrations of alpha 1 Acid Glycoprotein were measured in duplicates, interpolated from the alpha 1 Acid Glycoprotein standard curves and corrected for sample dilution. Undiluted samples are as follows : urine 1 : 800, milk 1 : 500, saliva 1 : 800 and HepG2 cell culture supernatant 3.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean alpha 1 Acid Glycoprotein concentration was determined to be 6.85 μg/mL in neat urine, 5.34 μg/mL in neat milk, 8.42 μg/mL in neat saliva and 0.23 μg/mL in neat HepG2 cell culture supernatant.

Sandwich ELISA - Human alpha 1 Acid Glycoprotein ELISA Kit (AB243675)
  • sELISA

Supplier Data

Sandwich ELISA - Human alpha 1 Acid Glycoprotein ELISA Kit (AB243675)

Interpolated concentrations of native alpha 1 Acid Glycoprotein in human serum and plasma samples.

The concentrations of alpha 1 Acid Glycoprotein were measured in duplicates, interpolated from the alpha 1 Acid Glycoprotein standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 80,000, plasma (citrate) 40,000, plasma (EDTA) 1 : 80,000 and plasma (heparin) 1 : 80,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean alpha 1 Acid Glycoprotein concentration was determined to be 821.2 μg/mL in neat serum, 479.5 μg/mL in neat plasma (citrate), 881.7 μg/mL in neat plasma (EDTA) and 767.8 μg/mL in neat plasma (heparin).

Sandwich ELISA - Human alpha 1 Acid Glycoprotein ELISA Kit (AB243675)
  • sELISA

Supplier Data

Sandwich ELISA - Human alpha 1 Acid Glycoprotein ELISA Kit (AB243675)

Example of human alpha 1 Acid Glycoprotein standard curve in Sample Diluent NS.

The alpha 1 Acid Glycoprotein standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human alpha 1 Acid Glycoprotein ELISA Kit (AB243675)
  • sELISA

Supplier Data

Sandwich ELISA - Human alpha 1 Acid Glycoprotein ELISA Kit (AB243675)

Serum from ten individual healthy human male donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean alpha 1 Acid Glycoprotein concentration was determined to be 777.1 μg/mL with a range of 518.6 – 1,117 μg/mL.

Sandwich ELISA - Human alpha 1 Acid Glycoprotein ELISA Kit (AB243675)
  • sELISA

Unknown

Sandwich ELISA - Human alpha 1 Acid Glycoprotein ELISA Kit (AB243675)

Human alpha 1 Acid Glycoprotein Standard Curve Comparison.

Standard Curve comparison between human alpha 1 Acid Glycoprotein SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

Key facts

Detection method

Colorimetric

Sample types

Saliva, Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Milk, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 68.5 pg/mL

Range

0.19 - 12 ng/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human alpha 1 Acid Glycoprotein ELISA Kit ab243675 is a rapid single-wash 90-min Sandwich ELISA to measure Human alpha 1 Acid Glycoprotein in cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, milk, saliva, serum, urine. This SimpleStep sensitivity is 68.5 pg/mL.

How the assay works

Human alpha 1 Acid Glycoprotein SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human alpha 1 Acid Glycoprotein ELISA Kit ab243675 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-Alpha 1 Acid Glycoprotein/AGP antibody [EPR21185-101] - BSA and Azide free (Capture) ab259512
- Anti-Alpha 1 Acid Glycoprotein/AGP antibody [EPR21185-110] - BSA and Azide free (Detector) ab259512

Alpha 1 Acid Glycoprotein is a secreted glycoprotein that functions as transport protein in the blood stream. It binds various ligands in the interior of its beta-barrel domain. It also binds synthetic drugs and influences their distribution and availability in the body. Alpha 1 Acid Glycoprotein appears to function in modulating the activity of the immune system during the acute-phase reaction.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.4" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.9" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "93 - 106 %", "average": "= 99" }, { "sample": "Serum", "range": "85 - 97 %", "average": "= 90" }, { "sample": "Milk", "range": "79 - 104 %", "average": "= 94" }, { "sample": "EDTA Plasma", "range": "105 - 108 %", "average": "= 107" }, { "sample": "Urine", "range": "93 - 112 %", "average": "= 103" }, { "sample": "Saliva", "range": "110 - 117 %", "average": "= 112" }, { "sample": "Heparin Plasma", "range": "100 - 112 %", "average": "= 106" }, { "sample": "Citrate plasma", "range": "88 - 105 %", "average": "= 95" }, { "sample": "Cell culture media", "range": "78 - 89 %", "average": "= 84" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab243675-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB243675-CMP07", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB243675-CMP10", "productcode":"" }, { "size":"2 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB243675-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB243675-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB243675-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human alpha 1 Acid Glycoprotein Lyophilized Recombinant Protein", "number":"AB243675-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human alpha 1 Acid Glycoprotein Capture Antibody", "number":"AB243675-CMP02", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB243675-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB243675-CMP06", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human alpha 1 Acid Glycoprotein Detector Antibody", "number":"AB243675-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha 1 Acid Glycoprotein also known as AGP or Alpha 1 Glycoprotein is a plasma protein with a molecular weight of approximately 41 to 43 kDa. This protein circulates primarily in the bloodstream and is produced mainly by the liver. AGP is known for its highly glycosylated structure carrying several branched oligosaccharide chains. It exhibits a significant capacity to bind various endogenous and exogenous lipophilic substances influencing their transport and distribution in the body.
Biological function summary

AGP plays a role in modulating the immune response and maintaining homeostasis. As an acute-phase protein its levels rise significantly during inflammation infection and trauma where it acts to modulate the immune system. AGP does not exist as part of a larger protein complex but interacts with plasma lipoproteins and other glycoproteins aiding in its function of maintaining vascular integrity during acute-phase reactions.

Pathways

Alpha 1 Glycoprotein serves as an important component in the inflammatory response and drug-binding pathways. In the inflammatory pathway AGP interacts with cytokines and acute-phase reactants adjusting the body's inflammatory processes. It is also involved in the binding and distribution of drugs interacting with albumin and other transport proteins to influence the pharmacokinetics of many medications.

AGP levels change in response to conditions like rheumatoid arthritis and liver diseases. In rheumatoid arthritis its increased levels correlate with disease activity and inflammation. In liver diseases the production and plasma concentration of AGP can reflect hepatic synthesis capability. AGP's interaction with cytokines in these conditions often links it to elevated levels of other acute-phase proteins like C-reactive protein reflecting its participation in the pathophysiological processes of inflammation.
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Product protocols

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Target data

Functions as a transport protein in the blood stream. Binds various ligands in the interior of its beta-barrel domain. Also binds synthetic drugs and influences their distribution and availability in the body. Appears to function in modulating the activity of the immune system during the acute-phase reaction.
See full target information ORM1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Diagnostics (Basel, Switzerland) 12: PubMed35741222

2022

Urine Proteome in Distinguishing Hepatic Steatosis in Patients with Metabolic-Associated Fatty Liver Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Chang-Hai Liu,Shanshan Zheng,Shisheng Wang,Dongbo Wu,Wei Jiang,Qingmin Zeng,Yi Wei,Yong Zhang,Hong Tang

Clinical kidney journal 14:1798-1807 PubMed34221387

2021

Pro-calcifying analysis of uraemic serum from patients treated with medium cut-off membrane in a prospective, cross-over study.

Applications

Unspecified application

Species

Unspecified reactive species

Paola Ciceri,Giorgia Tettamanti,Andrea Galassi,Lorenza Magagnoli,Nicolas Fabresse,Jean-Claude Alvarez,Ziad A Massy,Piergiorgio Messa,Mario Cozzolino
View all publications
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