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AB326079

Human Alpha 1 Acid Glycoprotein ELISA Kit, Chemiluminescent

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Human Alpha 1 Acid Glycoprotein ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Human Alpha 1 Acid Glycoprotein with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 0.09 - 192 ng/ml with a sensitivity of 0.05 pg/mL.
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
- Patent pending

View Alternative Names

AGP1, ORM1, Alpha-1-acid glycoprotein 1, AGP 1, Orosomucoid-1, OMD 1

6 Images
Sandwich ELISA - Human Alpha 1 Acid Glycoprotein ELISA Kit, Chemiluminescent (AB326079)
  • sELISA

Lab

Sandwich ELISA - Human Alpha 1 Acid Glycoprotein ELISA Kit, Chemiluminescent (AB326079)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

Sandwich ELISA - Human Alpha 1 Acid Glycoprotein ELISA Kit, Chemiluminescent (AB326079)
  • sELISA

Lab

Sandwich ELISA - Human Alpha 1 Acid Glycoprotein ELISA Kit, Chemiluminescent (AB326079)

Interpolated concentrations of native alpha 1 Acid Glycoprotein in human serum and plasma samples. The concentrations of alpha 1 Acid Glycoprotein were measured in duplicates, interpolated from the alpha 1 Acid Glycoprotein standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 80,000, plasma (citrate) 40,000, plasma (EDTA) 1 : 80,000 and plasma (heparin) 1 : 80,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean alpha 1 Acid Glycoprotein concentration was determined to be 821.2 µg/mL in neat serum, 479.5 µg/mL in neat plasma (citrate), 881.7 µg/mL in neat plasma (EDTA) and 767.8 µg/mL in neat plasma (heparin).

Sandwich ELISA - Human Alpha 1 Acid Glycoprotein ELISA Kit, Chemiluminescent (AB326079)
  • sELISA

Lab

Sandwich ELISA - Human Alpha 1 Acid Glycoprotein ELISA Kit, Chemiluminescent (AB326079)

Serum from ten individual healthy human male donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean alpha 1 Acid Glycoprotein concentration was determined to be 777.1 µg/mL with a range of 518.6 – 1,117 µg/mL.

Sandwich ELISA - Human Alpha 1 Acid Glycoprotein ELISA Kit, Chemiluminescent (AB326079)
  • sELISA

Lab

Sandwich ELISA - Human Alpha 1 Acid Glycoprotein ELISA Kit, Chemiluminescent (AB326079)

Dynamic range comparison between the Chemiluminescent Human alpha 1 Acid Glycoprotein SimpleStep ELISA, colorimetric Human alpha 1 Acid Glycoprotein SimpleStep ELISA (ab243675), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and dynamic range.

Sandwich ELISA - Human Alpha 1 Acid Glycoprotein ELISA Kit, Chemiluminescent (AB326079)
  • sELISA

Lab

Sandwich ELISA - Human Alpha 1 Acid Glycoprotein ELISA Kit, Chemiluminescent (AB326079)

Standard curve comparison between the Chemiluminescent Human alpha 1 Acid Glycoprotein SimpleStep ELISA and colorimetric Human alpha 1 Acid Glycoprotein SimpleStep ELISA (ab243675) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Alpha 1 Acid Glycoprotein ELISA Kit, Chemiluminescent (AB326079)
  • sELISA

Lab

Sandwich ELISA - Human Alpha 1 Acid Glycoprotein ELISA Kit, Chemiluminescent (AB326079)

Interpolated concentrations of native alpha 1 Acid Glycoprotein in human urine, milk, saliva and HepG2 cell culture supernatant samples. The concentrations of alpha 1 Acid Glycoprotein were measured in duplicates, interpolated from the alpha 1 Acid Glycoprotein standard curves and corrected for sample dilution. Undiluted samples are as follows : urine 1 : 800, milk 1 : 500, saliva 1 : 800 and HepG2 cell culture supernatant 3.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean alpha 1 Acid Glycoprotein concentration was determined to be 6.85 µg/mL in neat urine, 5.34 µg/mL in neat milk, 8.42 µg/mL in neat saliva and 0.23 µg/mL in neat HepG2 cell culture supernatant.

Key facts

Detection method

Luminescent

Sample types

Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture supernatant, Urine, Saliva, Milk, Cell culture media

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.05 pg/mL

Range

0.09 - 192 ng/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

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Product details

Human Alpha 1 Acid Glycoprotein ELISA Kit, Chemiluminescent (ab326079) is a rapid single-wash sandwich 55-min ELISA to measure Human Alpha 1 Acid Glycoprotein in Serum, Citrate Plasma, EDTA Plasma, Herpain Plasma, Cell Culture Supernatant, Urine, Saliva, Milk, Cell Culture Media with a sensitivity of 0.05 pg/mL.

How the assay works

Human Alpha 1 Acid Glycoprotein ELISA Kit, Chemiluminescent (ab326079) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human Alpha 1 Acid Glycoprotein (ab326079) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-Human alpha 1-Acid Glycoprotein antibody [EPR21185-101] - BSA and Aside free (Capture) ab259511
- Anti-Human alpha 1-Acid Glycoprotein antibody [EPR21185-110] - BSA and Aside free (Detector) ab259512
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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 5.9" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 5.4" } ]
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Recovery

[ { "sample": "Serum", "range": "85 - 97 %", "average": "= 90" }, { "sample": "Heparin Plasma", "range": "100 - 112 %", "average": "= 106" }, { "sample": "EDTA Plasma", "range": "105 - 108 %", "average": "= 107" }, { "sample": "Citrate plasma", "range": "88 - 105 %", "average": "= 95" }, { "sample": "Cell culture supernatant", "range": "93 - 106 %", "average": "= 99" }, { "sample": "Urine", "range": "93 - 112 %", "average": "= 103" }, { "sample": "Saliva", "range": "110 - 117 %", "average": "= 112" }, { "sample": "Milk", "range": "79 - 104 %", "average": "= 94" }, { "sample": "Cell culture media", "range": "78 - 89 %", "average": "= 84" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Functions as a transport protein in the blood stream. Binds various ligands in the interior of its beta-barrel domain. Also binds synthetic drugs and influences their distribution and availability in the body. Appears to function in modulating the activity of the immune system during the acute-phase reaction.
See full target information ORM1
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websiteProtocolBooklet
en
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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