AB229417

Human alpha 1 Antitrypsin ELISA Kit, Fluorescent

CatchPoint SimpleStep ELISA
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(1 Publication)

Human alpha 1 Antitrypsin ELISA Kit, Fluorescent is a single-wash 90-min Simplestep used to quantify Human alpha 1 Antitrypsin with a sensitivity of 5.8 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Fluorescent Sandwich ELISA - 530/570/590 nm readout : works on any standard plate reader

View Alternative Names

AAT, PI, PRO0684, PRO2209, SERPINA1, Alpha-1-antitrypsin, Alpha-1 protease inhibitor, Alpha-1-antiproteinase, Serpin A1

3 Images

Sandwich ELISA - Human alpha 1 Antitrypsin ELISA Kit, Fluorescent (AB229417)

sELISA

Unknown

Sandwich ELISA - Human alpha 1 Antitrypsin ELISA Kit, Fluorescent (AB229417)

Observed alpha 1 Antitrypsin levels in individual donor normal Human serum (n=10).

The mean alpha 1 Antitrypsin concentration was determined to be 72 mg/mL with a range of 17-181 mg/mL.

sELISA

Unknown

Sandwich ELISA - Human alpha 1 Antitrypsin ELISA Kit, Fluorescent (AB229417)

Titration of Human serum within the working range of the assay.

Background-subtracted data values (mean +/- SD, n = 2) are graphed.

sELISA

Unknown

Sandwich ELISA - Human alpha 1 Antitrypsin ELISA Kit, Fluorescent (AB229417)

Example of human alpha 1 Antitrypsin (SERPINA1) standard curve in Sample Diluent NS. The alpha 1 Antitrypsin (SERPINA1) standard curve was prepared as described in Section 10.

Key facts

Detection method

Fluorescent

Sample types

Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 5.8 ng/mL

Range

7.8 - 2000 ng/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

alpha 1 Antitrypsin (SERPINA1) in vitro CatchPoint SimpleStep ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of alpha 1 Antitrypsin (SERPINA1) protein in human serum and plasma.

This CatchPoint SimpleStep ELISA kit has been optimized for Molecular Devices Microplate Readers. Click here for a list of recommended Microplate Readers.
If using a Molecular Devices' plate reader supported by SoftMax® Pro software, a preconfigured protocol for these CatchPoint SimpleStep ELISA Kits is available with all the protocol and analysis settings at www.softmaxpro.org.

The CatchPoint SimpleStep ELISA employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. CatchPoint HRP Development Solution containing the Stoplight Red Substrate is added. During incubation, the substrate is catalyzed by HRP generating a fluorescent product. Signal is generated proportionally to the amount of bound analyte and the intensity is measured in a fluorescence plater reader at 530/570/590 nm Excitation/Cutoff/Emission.

Alpha 1 Antitrypsin (SERPINA1) is a serine protease inhibitor found in a variety of biological fluids. While it can inhibit trypsin, chymotrypsin, and plasminogen activator, its main target is elastase. Alpha 1 Antitrypsin plays a critical role in the lung by inhibiting the activity of elastase, produced by neutrophils during inflammatory responses. The aberrant form plays a role in coagulation and insulin induced NO synthesis. Alpha 1 Antitrypsin deficiency results in emphysema and may also result in liver disease.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Human Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.1" }, { "reproducibilityType": "Intra", "sample": "Human Serum", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.9" } ]

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Recovery

[ { "sample": "Serum", "range": "92 - 101 %", "average": "= 96" }, { "sample": "EDTA Plasma", "range": "70 - 87 %", "average": "= 77" }, { "sample": "Heparin Plasma", "range": "94 - 102 %", "average": "= 99" }, { "sample": "Citrate plasma", "range": "83 - 86 %", "average": "= 85" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha 1 Antitrypsin also known as A1AT or alpha-1 proteinase inhibitor is a serine protease inhibitor with a molecular mass of about 52 kDa. This protein mainly expresses in the liver and found in high concentrations in the blood plasma. A1AT protects tissues from enzymes of inflammatory cells especially neutrophil elastase. Its expression level is regulated by the liver making it a significant player in maintaining tissue integrity.

Biological function summary

A1AT regulates protease activity by forming complexes with target enzymes. It specifically inhibits neutrophil elastase a powerful enzyme capable of degrading elastin an important component of connective tissues. A1AT prevents excessive tissue damage during inflammation by maintaining a balance in protease activity within connective tissues across various organs.

Pathways

Alpha 1 Antitrypsin functions within the proteolytic pathways involved in inflammatory response and tissue remodeling. A1AT is closely related to neutrophil elastase in these processes. It interacts with other protease inhibitors like alpha 2-macroglobulin reinforcing its protective role against enzymatic activity that can lead to tissue destruction under pathophysiological conditions.

Alpha 1 Antitrypsin deficiency is a genetic condition that can cause chronic obstructive pulmonary disease (COPD) and liver cirrhosis. Deficient A1AT levels result in unregulated elastase activity leading to lung tissue damage and impaired liver function. Other proteins such as MMP-9 collaborate with elastase in exacerbating tissue damage illustrating how insufficient A1AT can significantly contribute to disease development.

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Product protocols

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Target data

Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin.. Short peptide from AAT. Reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE).

See full target information SERPINA1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Biomedical reports 16:43 PubMed35371477

2022

Phenotypical, functional and transcriptomic comparison of two modified methods of hepatocyte differentiation from human induced pluripotent stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Rong Li,Yang Zhao,Jeffrey J Yourick,Robert L Sprando,Xiugong Gao

View all publications

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websiteProtocolBooklet

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Human alpha 1 Antitrypsin ELISA Kit, Fluorescent

Sandwich ELISA - Human alpha 1 Antitrypsin ELISA Kit, Fluorescent (AB229417)

Sandwich ELISA - Human alpha 1 Antitrypsin ELISA Kit, Fluorescent (AB229417)

Sandwich ELISA - Human alpha 1 Antitrypsin ELISA Kit, Fluorescent (AB229417)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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