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AB108799

Human alpha 1 Antitrypsin ELISA Kit (SERPINA1)

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(1 Review)

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(26 Publications)

Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) is a sandwich ELISA designed to quantify Human alpha 1 Antitrypsin (SERPIN A1) with a sensitivity of 0.26 ng/mL.

- Wide dynamic range – quantifies 0.4–25 ng/mL
- Compatible with multiple sample types – works with serum, plasma, cerebral spinal fluid (CSF), milk and urine
- Assay platform – pre-coated microplate (12 x 8-well strips) for easy setup and reliable results
- Cited in over 20 publications

View Alternative Names

AAT, PI, PRO0684, PRO2209, SERPINA1, Alpha-1-antitrypsin, Alpha-1 protease inhibitor, Alpha-1-antiproteinase, Serpin A1

4 Images
ELISA - Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) (AB108799)
  • ELISA

Lab

ELISA - Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) (AB108799)

ELISA : alpha 1 Antitrypsin (SERPINA1) Human ELISA Kit.

Linearity : alpha 1 antitrypsin in serum and plasma (Li-heparine) at different diutions, corrected for the factor of dilution (duplicates +/- SD)

ELISA - Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) (AB108799)
  • ELISA

Lab

ELISA - Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) (AB108799)

ELISA : alpha 1 Antitrypsin (SERPINA1) Human ELISA Kit.

Alpha 1 antitrypsin in human biological fluids (duplicates +/- SD).

The samples were tested in different ranges of dilutions (serum and Li-heparine plasma : 2e3-1e6; CSF : 5e3-5e4; milk : 1e2-1e3; saliva : 2e2-2e3).

ELISA - Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) (AB108799)
  • ELISA

Lab

ELISA - Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) (AB108799)

ELISA : alpha 1 Antitrypsin (SERPINA1) Human ELISA Kit.

Linearity : alpha 1 antitrypsin in urine at different diutions, corrected for the factor of dilution (duplicates +/- SD).

ELISA - Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) (AB108799)
  • ELISA

Lab

ELISA - Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) (AB108799)

ELISA : alpha 1 Antitrypsin (SERPINA1) Human ELISA Kit.

Linearity : alpha 1 antitrypsin in human milk at different diutions, corrected for the factor of dilution (duplicates +/- SD)

Key facts

Detection method

Colorimetric

Sample types

Cerebral Spinal Fluid, Milk, Plasma, Saliva, Serum, Urine

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.22 ng/mL

Range

0.391 - 25 ng/mL

Assay time

4h

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) ab108799 is a sanwich ELISA to measure Human alpha 1 Antitrypsin (SERPIN A1) in Cerebral Spinal Fluid (CSF), Milk, Plasma, Saliva, Serum, Urine with a sensitivity of 0.26 ng/mL.

How the assay works

An alpha 1 antitrypsin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an alpha 1 antitrypsin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of alpha 1 antitrypsin captured in plate.

Assay specificity

The standard has been calibrated against WHO International Standard.

Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) ab108799 protocol summary

1. Add standards or samples to the wells, incubate 2 hrs
2. Wash, then add 50 µl of Biotinylated Antibody per well. Incubate 1 hr
3. Wash, then add 50 µl of SP Conjugate per well. Incubate 30 min
4. Wash, then add 50 µl of Chromogen Substrate per well. Incubate 20 min
5. Add 50 µl of Stop Solution per well. Read at 450 nm immediately

How other researchers are using Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) ab108799

Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) ab108799 has been used in publications to develop liver organoids from human PSCs (1), urinary biomarker panels for endometriosis (2), and mRNA therapies for alpha 1-antitrypsin deficiency (3).

References:

(1)Gareth J Sullivan et al. 2023 PMID: 37653039,
(2)Ting-Chang Chang et al. 2022 PMID: 35453583,
(3)Ewa Ellis et al. 2020 PMID: 32341402

Get results in 90 minutes our SimpleStep ELISA® kit:

- Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) ab189579

Related and recommended products

Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) ab108799 is commonly used to study liver function and inflammation.

Other kits for measuring liver function and inflammation include:

- Human Albumin ELISA kit ab179887
- Human ALT ELISA kit ab234578
- Human AST ELISA Kit ab263881

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.4" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.3" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab108799-1x96Tests", "assetComponentDetails": [ { "size":"2 x 30 mL", "name":"20X Wash Buffer Concentrate", "number":"AB108799-CMP06", "productcode":"" }, { "size":"1 x 120 µL", "name":"50X Biotinylated Human alpha 1 Antitrypsin Antibody", "number":"AB108799-CMP01", "productcode":"" }, { "size":"1 x 30 mL", "name":"10X Diluent N Concentrate", "number":"AB108799-CMP02", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Solution", "number":"AB108799-CMP08", "productcode":"" }, { "size":"1 x 7 mL", "name":"Chromogen Substrate", "number":"AB108799-CMP03", "productcode":"" }, { "size":"1 x 1 Vial", "name":"alpha 1 Antitrypsin Standard", "number":"AB108799-CMP05", "productcode":"" }, { "size":"3 x 1 Unit", "name":"Sealing Tapes", "number":"AB108799-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"alpha 1 Antitrypsin Microplate (12 x 8 well strips)", "number":"AB108799-CMP04", "productcode":"" }, { "size":"1 x 80 µL", "name":"100X Streptavidin-Peroxidase Conjugate", "number":"AB108799-CMP09", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha 1 Antitrypsin also known as A1AT or alpha-1 proteinase inhibitor is a serine protease inhibitor with a molecular mass of about 52 kDa. This protein mainly expresses in the liver and found in high concentrations in the blood plasma. A1AT protects tissues from enzymes of inflammatory cells especially neutrophil elastase. Its expression level is regulated by the liver making it a significant player in maintaining tissue integrity.
Biological function summary

A1AT regulates protease activity by forming complexes with target enzymes. It specifically inhibits neutrophil elastase a powerful enzyme capable of degrading elastin an important component of connective tissues. A1AT prevents excessive tissue damage during inflammation by maintaining a balance in protease activity within connective tissues across various organs.

Pathways

Alpha 1 Antitrypsin functions within the proteolytic pathways involved in inflammatory response and tissue remodeling. A1AT is closely related to neutrophil elastase in these processes. It interacts with other protease inhibitors like alpha 2-macroglobulin reinforcing its protective role against enzymatic activity that can lead to tissue destruction under pathophysiological conditions.

Alpha 1 Antitrypsin deficiency is a genetic condition that can cause chronic obstructive pulmonary disease (COPD) and liver cirrhosis. Deficient A1AT levels result in unregulated elastase activity leading to lung tissue damage and impaired liver function. Other proteins such as MMP-9 collaborate with elastase in exacerbating tissue damage illustrating how insufficient A1AT can significantly contribute to disease development.
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Product protocols

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Target data

Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin.. Short peptide from AAT. Reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE).
See full target information SERPINA1
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Publications (26)

Recent publications for all applications. Explore the full list and refine your search

Journal of ovarian research 18:109 PubMed40420300

2025

a1-antitrypsin, a new biomarker of polycystic ovary syndrome by changing its expression and rhythm.

Applications

Unspecified application

Species

Unspecified reactive species

Na Li,Beilei Shen,Wei Cao,RouRou Chen,Rongbo He,Li Qian,Lin Xu,Yu Liu

Molecular vision 30:137-149 PubMed39377095

2024

The effect of age on aqueous humor of humans with high myopia.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Wen,Mengjun Fu,Yongtao Li,Haorun Zhang,Xiu Wang,Yang Cai,Yaoling Li,Ruihong Su,Yifang Huang,Ming Liu,Yufeng Zhang,Shaozhen Zhao,Jing Sun

Experimental & molecular medicine 55:2005-2024 PubMed37653039

2023

Scalable production of tissue-like vascularized liver organoids from human PSCs.

Applications

Unspecified application

Species

Unspecified reactive species

Sean P Harrison,Richard Siller,Yoshiaki Tanaka,Maria Eugenia Chollet,María Eugenia de la Morena-Barrio,Yangfei Xiang,Benjamin Patterson,Elisabeth Andersen,Carlos Bravo-Pérez,Henning Kempf,Kathrine S Åsrud,Oleg Lunov,Alexandr Dejneka,Marie-Christine Mowinckel,Benedicte Stavik,Per Morten Sandset,Espen Melum,Saphira Baumgarten,Flavio Bonanini,Dorota Kurek,Santosh Mathapati,Runar Almaas,Kulbhushan Sharma,Steven R Wilson,Frøydis S Skottvoll,Ida C Boger,Inger Lise Bogen,Tuula A Nyman,Jun Jie Wu,Ales Bezrouk,Dana Cizkova,Javier Corral,Jaroslav Mokry,Robert Zweigerdt,In-Hyun Park,Gareth J Sullivan

mBio 13:e0089222 PubMed35532162

2022

Serine Protease Inhibitors Restrict Host Susceptibility to SARS-CoV-2 Infections.

Applications

Unspecified application

Species

Unspecified reactive species

Ebba Rosendal,Ionut Sebastian Mihai,Miriam Becker,Debojyoti Das,Lars Frängsmyr,B David Persson,Gregory D Rankin,Remigius Gröning,Johan Trygg,Mattias Forsell,Johan Ankarklev,Anders Blomberg,Johan Henriksson,Anna K Överby,Annasara Lenman

Biomedicines 10: PubMed35453583

2022

Urinary Biomarkers for Detection of Clinical Endometriosis or Adenomyosis.

Applications

Unspecified application

Species

Unspecified reactive species

Wei-Chun Chen,Chao-Min Cheng,Wan-Ting Liao,Ting-Chang Chang

Cells 10: PubMed34065411

2021

Establishing a 3D In Vitro Hepatic Model Mimicking Physiologically Relevant to In Vivo State.

Applications

Unspecified application

Species

Unspecified reactive species

Hyun Kyoung Kang,Madina Sarsenova,Da-Hyun Kim,Min Soo Kim,Jin Young Lee,Eun-Ah Sung,Myung Geun Kook,Nam Gyo Kim,Soon Won Choi,Vyacheslav Ogay,Kyung-Sun Kang

Cell reports 33:108453 PubMed33264615

2020

The Microfluidic Environment Reveals a Hidden Role of Self-Organizing Extracellular Matrix in Hepatic Commitment and Organoid Formation of hiPSCs.

Applications

Unspecified application

Species

Unspecified reactive species

Federica Michielin,Giovanni G Giobbe,Camilla Luni,Qianjiang Hu,Ida Maroni,Michael R Orford,Anna Manfredi,Lucio Di Filippo,Anna L David,Davide Cacchiarelli,Paolo De Coppi,Simon Eaton,Nicola Elvassore

Malaria journal 19:214 PubMed32571333

2020

Plasmodium vivax liver stage assay platforms using Indian clinical isolates.

Applications

Unspecified application

Species

Unspecified reactive species

Pradeep A Subramani,Neha Vartak-Sharma,Seetha Sreekumar,Pallavi Mathur,Bhavana Nayer,Sushrut Dakhore,Sowmya K Basavanna,Devaiah M Kalappa,Ramya V Krishnamurthy,Benudhar Mukhi,Priyasha Mishra,Noriko Yoshida,Susanta Kumar Ghosh,Radhakrishan Shandil,Shridhar Narayanan,Brice Campo,Kouichi Hasegawa,Anupkumar R Anvikar,Neena Valecha,Varadharajan Sundaramurthy

Scientific reports 10:7052 PubMed32341402

2020

Systemic modified messenger RNA for replacement therapy in alpha 1-antitrypsin deficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Ahmad Karadagi,Alex G Cavedon,Helen Zemack,Greg Nowak,Marianne E Eybye,Xuling Zhu,Eleonora Guadagnin,Rebecca A White,Lisa M Rice,Andrea L Frassetto,Stephen Strom,Carl Jorns,Paolo G V Martini,Ewa Ellis

Nitric oxide : biology and chemistry 95:1-11 PubMed31778801

2019

Saliva panel of protein candidates: A comprehensive study for assessing high altitude acclimatization.

Applications

Unspecified application

Species

Unspecified reactive species

Shikha Jain,Subhojit Paul,Ram Niwas Meena,Anamika Gangwar,Usha Panjwani,Yasmin Ahmad,Kalpana Bhargava
View all publications
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