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AB189579

Human alpha 1 Antitrypsin ELISA Kit (SERPINA1)

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(12 Publications)

Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) is a single-wash 90-min Simplestep used to quantify Human alpha 1 Antitrypsin (SERPINA1) with a sensitivity of 13 ng/mL. Suitable to work with Heparin Plasma, Citrate plasma, Serum, EDTA Plasma samples. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput.
- Cited in over 10 citations

View Alternative Names

AAT, PI, PRO0684, PRO2209, SERPINA1, Alpha-1-antitrypsin, Alpha-1 protease inhibitor, Alpha-1-antiproteinase, Serpin A1

6 Images
ELISA - Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) (AB189579)
  • ELISA

Supplier Data

ELISA - Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) (AB189579)
Sandwich ELISA - Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) (AB189579)
  • sELISA

Supplier Data

Sandwich ELISA - Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) (AB189579)

Example of human alpha 1 Antitrypsin standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) (AB189579)
  • sELISA

Supplier Data

Sandwich ELISA - Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) (AB189579)

Serum of eight individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean alpha 1 Antitrypsin concentration was determined to be 0.73 mg/mL with a range of 0.22 – 2.39 mg/mL.

Sandwich ELISA - Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) (AB189579)
  • sELISA

Supplier Data

Sandwich ELISA - Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) (AB189579)

Interpolated concentration of native alpha 1 Antitrypsin was measured in duplicate at different sample concentrations. Undiluted samples are as follows : serum 1 : 4,000, plasma (citrate)1 : 2,000, plasma (EDTA) 1 : 8,000, and plasma (heparin) 1 : 8,000. The interpolated dilution factor corrected values (to neat) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) (AB189579)
  • sELISA

Supplier Data

Sandwich ELISA - Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) (AB189579)

Example of human alpha 1 Antitrypsin standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) (AB189579)
  • sELISA

Supplier Data

Sandwich ELISA - Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) (AB189579)

Interpolated concentration of native alpha 1 Antitrypsin was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1 : 2,500 serum.  The interpolated dilution factor corrected values (to neat) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 13 ng/mL

Range

31.25 - 2000 ng/mL

Assay time

1h 30m

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) ab189579 is a rapid single-wash 90-min ELISA kit to measure Human alpha 1 Antitrypsin in serum and plasma. This SimpleStep ELISA® sensitivity is 0.02 ng/mL.

How the assay works

Human alpha 1 Antitrypsin SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Human IgM, human IgA and human IgE were prepared at 10 ng/mL and 250 ng/mL in Sample Diluent NS and assayed for cross reactivity. No cross-reactivity was observed for IgM or IgE at either concentration with a mean OD deviation from background of -0.01. No cross-reactivity was observed for IgA at 10 ng/mL and only 0.4% cross-reactivity at 250 ng/mL of IgA.
Purified human IgG was assayed at 5 ng/mL in the presence and absence of 250 ng/mL of human IgM, human IgA and human IgE to determine interference. After background subtraction, recovery of human IgG was observed at a mean of 98% with a standard deviation of 0.06.

Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) ab189579 protocol summary:

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm

How other researchers have used Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) ab189579

Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) ab189579 has been used in publications to study bioengineered liver grafts for treating acute liver failure (1), alpha 1 antitrypsin for predicting acute kidney injury after cardiopulmonary bypass (2), and cystatin C correlation with alpha-1 antitrypsin in asthma(3).

References:

(1)Jeff J Ross et al. 2022 PMID: 37892931,
(2)Jun Ai et al. 2019 PMID: 32030221,
(3)Mohamad Sadikin et al. 2023 PMID: 38094096

Save your precious samples

Our 384-well Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) only requires a maxmum of 12.5µL

Related and recommended products

Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) ab189579 is commonly used to study liver function and inflammation.

Other kits for measuring liver function and inflammation include:

- Human Albumin ELISA kit ab179887
- Human ALT ELISA kit ab234578
- Human AST ELISA Kit ab263881

alpha 1 Antitrypsin (SERPINA1) is a serine protease inhibitor found in a variety of biological fluids. While it can inhibit trypsin, chymotrypsin, and plasminogen activator, it's main target is elastase. Alpha 1 antitrypsin plays a critical role in the lung by inhibiting the activity of elastase, produced by neutrophils during inflammatory responses. The aberrant form plays a role in coagulation and insulin induced NO synthesis. Alpha 1 antitrypsin deficiency results in emphysema and may also result in liver disease.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.1" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.9" } ]
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Recovery

[ { "sample": "Serum", "range": "92 - 101 %", "average": "= 96" }, { "sample": "EDTA Plasma", "range": "70 - 87 %", "average": "= 77" }, { "sample": "Heparin Plasma", "range": "94 - 102 %", "average": "= 99" }, { "sample": "Citrate plasma", "range": "83 - 86 %", "average": "= 85" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin.. Short peptide from AAT. Reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE).
See full target information SERPINA1
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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

Biomedicines 12: PubMed38927479

2024

First Application of a Mixed Porcine-Human Repopulated Bioengineered Liver in a Preclinical Model of Post-Resection Liver Failure.

Applications

Unspecified application

Species

Unspecified reactive species

Philipp Felgendreff,Seyed Mohammad Hosseiniasl,Anna Minshew,Bruce P Amiot,Silvana Wilken,Boyukkhanim Ahmadzada,Robert C Huebert,Nidhi Jalan Sakrikar,Noah G Engles,Peggy Halsten,Kendra Mariakis,John Barry,Shawn Riesgraf,Chris Fecteau,Jeffrey J Ross,Scott L Nyberg

Asia Pacific allergy 13:158-163 PubMed38094096

2023

Activities and concentration of alpha-1 antitrypsin and cystatin C in serum from patients with house dust mite asthma.

Applications

Unspecified application

Species

Unspecified reactive species

Mailani Dwi Hidayati,Febriana Catur Iswanti,Samsuridjal Djauzi,Sukamto Koesnoe,Mohamad Sadikin

Bioengineering (Basel, Switzerland) 10: PubMed37892931

2023

Manufacturing and Functional Characterization of Bioengineered Liver Grafts for Extracorporeal Liver Assistance in Acute Liver Failure.

Applications

Unspecified application

Species

Unspecified reactive species

Victoria L Nelson,Aron R Stumbras,R Noelle Palumbo,Shawn A Riesgraf,Marie S Balboa,Zachary A Hannah,Isaac J Bergstrom,Christopher J Fecteau,John R Lake,John J Barry,Jeff J Ross

Arthritis & rheumatology (Hoboken, N.J.) 75:1039-1047 PubMed36575650

2023

Neutrophil Extracellular Trap-Borne Elastase Prevents Inflammatory Relapse in Intercritical Gout.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Liu,Lizhen Shan,Han Wang,Christine Schauer,Janina Schoen,Lingjiang Zhu,Ci Lu,Zitao Wang,Yu Xue,Huaxiang Wu,Martin Herrmann

International journal of molecular sciences 23: PubMed35806409

2022

Alpha-1 Antitrypsin Reduces Disease Progression in a Mouse Model of Charcot-Marie-Tooth Type 1A: A Role for Decreased Inflammation and ADAM-17 Inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Nikolay Zhukovsky,Marianna Silvano,Thierry Filloux,Sergio Gonzalez,Karl-Heinz Krause

Frontiers in cell and developmental biology 8:550543 PubMed33015055

2020

SERPINA1 Gene Promoter Is Differentially Methylated in Peripheral Blood Mononuclear Cells of Pregnant Women.

Applications

Unspecified application

Species

Unspecified reactive species

John Charles Rotondo,Lucia Oton-Gonzalez,Rita Selvatici,Paola Rizzo,Rita Pavasini,Gianluca Calogero Campo,Carmen Lanzillotti,Chiara Mazziotta,Monica De Mattei,Mauro Tognon,Fernanda Martini

Journal of thoracic disease 11:5053-5062 PubMed32030221

2020

Serum alpha 1-antitrypsin predicts severe acute kidney injury after cardiac surgery.

Applications

Unspecified application

Species

Unspecified reactive species

Songlin Du,Jianwei Tian,Zhiwen Xiao,Zhiwen Luo,Tong Lin,Shaoyi Zheng,Jun Ai

The Journal of heart and lung transplantation : the official publication of the International Society for Heart Transplantation 37:656-666 PubMed29153638

2017

α-Anti-trypsin improves function of porcine donor lungs during ex-vivo lung perfusion.

Applications

Unspecified application

Species

Unspecified reactive species

Huiqing Lin,Manyin Chen,Feng Tian,Jussi Tikkanen,Lei Ding,Hei Yu Andrew Cheung,Daisuke Nakajima,Zhe Wang,Andrea Mariscal,David Hwang,Marcelo Cypel,Shaf Keshavjee,Mingyao Liu

Biotechnology journal 11:1648-1656 PubMed27731958

2016

Case study on human α1-antitrypsin: Recombinant protein titers obtained by commercial ELISA kits are inaccurate.

Applications

sELISA

Species

Human

Henning Gram Hansen,Helene Faustrup Kildegaard,Gyun Min Lee,Stefan Kol

Journal of advanced research 7:1019-1028 PubMed27857848

2016

The effect of α-antitrypsin deficiency combined with increased bacterial loads on chronic obstructive pulmonary disease pharmacotherapy: A prospective, parallel, controlled pilot study.

Applications

sELISA

Species

Human

Marwa G Hennawy,Noha M Elhosseiny,Hussein Sultan,Wael Abdelfattah,Yousry Akl,Nirmeen A Sabry,Ahmed S Attia
View all publications
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