Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) is a single-wash 90-min Simplestep used to quantify Human alpha 1 Antitrypsin (SERPINA1) with a sensitivity of 13 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Cited in over 10 citations
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Publications
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- Asia Pacific allergy 13:158-1632023Activities and concentration of alpha-1 antitrypsin and cystatin C in serum from patients with house dust mite asthma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMailani Dwi Hidayati,Febriana Catur Iswanti,Samsuridjal Djauzi,Sukamto Koesnoe,Mohamad SadikinPubMed 38094096
- Bioengineering (Basel, Switzerland) 10:2023Manufacturing and Functional Characterization of Bioengineered Liver Grafts for Extracorporeal Liver Assistance in Acute Liver Failure.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVictoria L Nelson,Aron R Stumbras,R Noelle Palumbo,Shawn A Riesgraf,Marie S Balboa,Zachary A Hannah,Isaac J Bergstrom,Christopher J Fecteau,John R Lake,John J Barry,Jeff J RossPubMed 37892931
- Arthritis & rheumatology (Hoboken, N.J.) 75:1039-10472023Neutrophil Extracellular Trap-Borne Elastase Prevents Inflammatory Relapse in Intercritical Gout.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLei Liu,Lizhen Shan,Han Wang,Christine Schauer,Janina Schoen,Lingjiang Zhu,Ci Lu,Zitao Wang,Yu Xue,Huaxiang Wu,Martin HerrmannPubMed 36575650
- International journal of molecular sciences 23:2022Alpha-1 Antitrypsin Reduces Disease Progression in a Mouse Model of Charcot-Marie-Tooth Type 1A: A Role for Decreased Inflammation and ADAM-17 Inhibition.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNikolay Zhukovsky,Marianna Silvano,Thierry Filloux,Sergio Gonzalez,Karl-Heinz KrausePubMed 35806409
- Frontiers in cell and developmental biology 8:5505432020SERPINA1 Gene Promoter Is Differentially Methylated in Peripheral Blood Mononuclear Cells of Pregnant Women.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJohn Charles Rotondo et. al.PubMed 33015055
- Journal of thoracic disease 11:5053-50622020Serum alpha 1-antitrypsin predicts severe acute kidney injury after cardiac surgery.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSonglin Du,Jianwei Tian,Zhiwen Xiao,Zhiwen Luo,Tong Lin,Shaoyi Zheng,Jun AiPubMed 32030221
- The Journal of heart and lung transplantation : the official publication of the International Society for Heart Transplantation 37:656-6662017α-Anti-trypsin improves function of porcine donor lungs during ex-vivo lung perfusion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuiqing Lin,Manyin Chen,Feng Tian,Jussi Tikkanen,Lei Ding,Hei Yu Andrew Cheung,Daisuke Nakajima,Zhe Wang,Andrea Mariscal,David Hwang,Marcelo Cypel,Shaf Keshavjee,Mingyao LiuPubMed 29153638
- Biotechnology journal 11:1648-16562016Case study on human α1-antitrypsin: Recombinant protein titers obtained by commercial ELISA kits are inaccurate.
- Journal of advanced research 7:1019-10282016The effect of α-antitrypsin deficiency combined with increased bacterial loads on chronic obstructive pulmonary disease pharmacotherapy: A prospective, parallel, controlled pilot study.Applications:sELISAReactive species:HumanMarwa G Hennawy,Noha M Elhosseiny,Hussein Sultan,Wael Abdelfattah,Yousry Akl,Nirmeen A Sabry,Ahmed S AttiaPubMed 27857848
- Analytical chemistry 87:7235-402015Direct Detection of α-1 Antitrypsin in Serum Samples using Surface Plasmon Resonance with a New Aptamer-Antibody Sandwich Assay.
Key facts
- Detection method
- Colorimetric
- Sample types
- Heparin Plasma, Citrate plasma, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 31.25 - 2000 ng/mL
- Assay time
- 1h 30m
- Sensitivity
- = 13 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin. Short peptide from AAT. Reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE).
Alternative names
AAT, PI, PRO0684, PRO2209, SERPINA1, Alpha-1-antitrypsin, Alpha-1 protease inhibitor, Alpha-1-antiproteinase, Serpin A1
What's included?
Recommended products
Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) is a single-wash 90-min Simplestep used to quantify Human alpha 1 Antitrypsin (SERPINA1) with a sensitivity of 13 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Cited in over 10 citations
Key facts
- Detection method
- Colorimetric
- Sample types
- Heparin Plasma, Citrate plasma, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 31.25 - 2000 ng/mL
- Assay time
- 1h 30m
- Assay Platform
- Microplate
- Sensitivity
- = 13 ng/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Overall | n 5 | C.V. 3.9 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Overall | n 3 | C.V. 2.1 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 96 | Range 92 - 101 % |
Sample type EDTA Plasma | Average % = 77 | Range 70 - 87 % |
Sample type Heparin Plasma | Average % = 99 | Range 94 - 102 % |
Sample type Citrate plasma | Average % = 85 | Range 83 - 86 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) ab189579 is a rapid single-wash 90-min ELISA kit to measure Human alpha 1 Antitrypsin in serum and plasma. This SimpleStep ELISA® sensitivity is 0.02 ng/mL.
How the assay works
Human alpha 1 Antitrypsin SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
Human IgM, human IgA and human IgE were prepared at 10 ng/mL and 250 ng/mL in Sample Diluent NS and assayed for cross reactivity. No cross-reactivity was observed for IgM or IgE at either concentration with a mean OD deviation from background of -0.01. No cross-reactivity was observed for IgA at 10 ng/mL and only 0.4% cross-reactivity at 250 ng/mL of IgA.
Purified human IgG was assayed at 5 ng/mL in the presence and absence of 250 ng/mL of human IgM, human IgA and human IgE to determine interference. After background subtraction, recovery of human IgG was observed at a mean of 98% with a standard deviation of 0.06.
Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) ab189579 protocol summary:
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm
How other researchers have used Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) ab189579
Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) ab189579 has been used in publications to study bioengineered liver grafts for treating acute liver failure (1), alpha 1 antitrypsin for predicting acute kidney injury after cardiopulmonary bypass (2), and cystatin C correlation with alpha-1 antitrypsin in asthma(3).
References:
(1)Jeff J Ross et al. 2022 PMID: 37892931,
(2)Jun Ai et al. 2019 PMID: 32030221,
(3)Mohamad Sadikin et al. 2023 PMID: 38094096
Save your precious samples
Our 384-well Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) only requires a maxmum of 12.5µL
Related and recommended products
Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) ab189579 is commonly used to study liver function and inflammation.
Other kits for measuring liver function and inflammation include:
- Human Albumin ELISA kit Human Albumin ELISA Kit ab179887
- Human ALT ELISA kit Human ALT ELISA Kit ab234578
- Human AST ELISA Kit Human AST ELISA Kit (Aspartate Aminotransferase) ab263881
alpha 1 Antitrypsin (SERPINA1) is a serine protease inhibitor found in a variety of biological fluids. While it can inhibit trypsin, chymotrypsin, and plasminogen activator, it's main target is elastase. Alpha 1 antitrypsin plays a critical role in the lung by inhibiting the activity of elastase, produced by neutrophils during inflammatory responses. The aberrant form plays a role in coagulation and insulin induced NO synthesis. Alpha 1 antitrypsin deficiency results in emphysema and may also result in liver disease.
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6 product images
ELISA - Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) (ab189579)
Sandwich ELISA - Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) (ab189579)
Interpolated concentration of native alpha 1 Antitrypsin was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1:2,500 serum. The interpolated dilution factor corrected values (to neat) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) (ab189579)
Serum of eight individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean alpha 1 Antitrypsin concentration was determined to be 0.73 mg/mL with a range of 0.22 – 2.39 mg/mL.
Sandwich ELISA - Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) (ab189579)
Interpolated concentration of native alpha 1 Antitrypsin was measured in duplicate at different sample concentrations. Undiluted samples are as follows: serum 1:4,000, plasma (citrate)1:2,000, plasma (EDTA) 1:8,000, and plasma (heparin) 1:8,000. The interpolated dilution factor corrected values (to neat) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) (ab189579)
Example of human alpha 1 Antitrypsin standard curve. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) (ab189579)
Example of human alpha 1 Antitrypsin standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
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