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Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) is a single-wash 90-min Simplestep used to quantify Human alpha 1 Antitrypsin (SERPINA1) with a sensitivity of 13 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Cited in over 10 citations

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Images

Sandwich ELISA - Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) (AB189579), expandable thumbnail
  • Sandwich ELISA - Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) (AB189579), expandable thumbnail
  • Sandwich ELISA - Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) (AB189579), expandable thumbnail
  • Sandwich ELISA - Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) (AB189579), expandable thumbnail
  • Sandwich ELISA - Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) (AB189579), expandable thumbnail

Publications

Key facts

Detection method
Colorimetric
Sample types
Heparin Plasma, Citrate plasma, Serum, EDTA Plasma
Assay type
Sandwich (quantitative)
Reactive species
Human
Range
31.25 - 2000 ng/mL
Assay time
1h 30m
Sensitivity
= 13 ng/mL

Reactivity data

Application
sELISA
Reactivity
Reacts
Dilution info
-
Notes

-

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Target data

Function

Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin. Short peptide from AAT. Reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE).

Alternative names

What's included?

1 x 384 Tests
Components
10X Human alpha 1 Antitrypsin Capture Antibody
1 x 600 µL
10X Human alpha 1 Antitrypsin Detector Antibody
1 x 600 µL
10X Wash Buffer PT (ab206977)
1 x 20 mL
384 well CaptSure™ microplates
1 x 1 Unit
Antibody Diluent 4BI
1 x 6 mL
Human alpha 1 Antitrypsin Lyophilized Purified Protein
2 x 1 Vial
Plate Seals
1 x 1 Unit
Sample Diluent NS (ab193972)
1 x 250 mL
Stop Solution
2 x 12 mL
TMB Development Solution
2 x 12 mL

Recommended products

Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) is a single-wash 90-min Simplestep used to quantify Human alpha 1 Antitrypsin (SERPINA1) with a sensitivity of 13 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Cited in over 10 citations

Key facts

Detection method
Colorimetric
Sample types
Heparin Plasma, Citrate plasma, Serum, EDTA Plasma
Assay type
Sandwich (quantitative)
Reactive species
Human
Range
31.25 - 2000 ng/mL
Assay time
1h 30m
Assay Platform
Microplate
Sensitivity
= 13 ng/mL

Precision

Intra assay

Sample
Overall
n
5
C.V.
3.9

Inter assay

Sample
Overall
n
3
C.V.
2.1

Recovery

Sample specific recovery

Sample type
Serum
Average %
= 96
Range
92 - 101 %
Sample type
EDTA Plasma
Average %
= 77
Range
70 - 87 %
Sample type
Heparin Plasma
Average %
= 99
Range
94 - 102 %
Sample type
Citrate plasma
Average %
= 85
Range
83 - 86 %

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C

Notes

Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) ab189579 is a rapid single-wash 90-min ELISA kit to measure Human alpha 1 Antitrypsin in serum and plasma. This SimpleStep ELISA® sensitivity is 0.02 ng/mL.

How the assay works

Human alpha 1 Antitrypsin SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Human IgM, human IgA and human IgE were prepared at 10 ng/mL and 250 ng/mL in Sample Diluent NS and assayed for cross reactivity. No cross-reactivity was observed for IgM or IgE at either concentration with a mean OD deviation from background of -0.01. No cross-reactivity was observed for IgA at 10 ng/mL and only 0.4% cross-reactivity at 250 ng/mL of IgA.
Purified human IgG was assayed at 5 ng/mL in the presence and absence of 250 ng/mL of human IgM, human IgA and human IgE to determine interference. After background subtraction, recovery of human IgG was observed at a mean of 98% with a standard deviation of 0.06.

Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) ab189579 protocol summary:

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm

How other researchers have used Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) ab189579

Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) ab189579 has been used in publications to study bioengineered liver grafts for treating acute liver failure (1), alpha 1 antitrypsin for predicting acute kidney injury after cardiopulmonary bypass (2), and cystatin C correlation with alpha-1 antitrypsin in asthma(3).

References:

(1)Jeff J Ross et al. 2022 PMID: 37892931,
(2)Jun Ai et al. 2019 PMID: 32030221,
(3)Mohamad Sadikin et al. 2023 PMID: 38094096

Save your precious samples

Our 384-well Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) only requires a maxmum of 12.5µL

Related and recommended products

Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) ab189579 is commonly used to study liver function and inflammation.

Other kits for measuring liver function and inflammation include:

- Human Albumin ELISA kit Human Albumin ELISA Kit ab179887
- Human ALT ELISA kit Human ALT ELISA Kit ab234578
- Human AST ELISA Kit Human AST ELISA Kit (Aspartate Aminotransferase) ab263881

alpha 1 Antitrypsin (SERPINA1) is a serine protease inhibitor found in a variety of biological fluids. While it can inhibit trypsin, chymotrypsin, and plasminogen activator, it's main target is elastase. Alpha 1 antitrypsin plays a critical role in the lung by inhibiting the activity of elastase, produced by neutrophils during inflammatory responses. The aberrant form plays a role in coagulation and insulin induced NO synthesis. Alpha 1 antitrypsin deficiency results in emphysema and may also result in liver disease.

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5 product images

  • Sandwich ELISA - Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) (ab189579), expandable thumbnail

    Sandwich ELISA - Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) (ab189579)

    Interpolated concentration of native alpha 1 Antitrypsin was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1:2,500 serum.  The interpolated dilution factor corrected values (to neat) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS. 

  • Sandwich ELISA - Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) (ab189579), expandable thumbnail

    Sandwich ELISA - Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) (ab189579)

    Serum of eight individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean alpha 1 Antitrypsin concentration was determined to be 0.73 mg/mL with a range of 0.22 – 2.39 mg/mL.

  • Sandwich ELISA - Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) (ab189579), expandable thumbnail

    Sandwich ELISA - Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) (ab189579)

    Interpolated concentration of native alpha 1 Antitrypsin was measured in duplicate at different sample concentrations. Undiluted samples are as follows: serum 1:4,000, plasma (citrate)1:2,000, plasma (EDTA) 1:8,000, and plasma (heparin) 1:8,000. The interpolated dilution factor corrected values (to neat) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

  • Sandwich ELISA - Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) (ab189579), expandable thumbnail

    Sandwich ELISA - Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) (ab189579)

    Example of human alpha 1 Antitrypsin standard curve. Background-subtracted data values (mean +/- SD) are graphed.

  • Sandwich ELISA - Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) (ab189579), expandable thumbnail

    Sandwich ELISA - Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) (ab189579)

    Example of human alpha 1 Antitrypsin standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed. 

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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