Human alpha 1 Antitrypsin ELISA Kit (SERPINA1)

Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) ab189579 is a rapid single-wash 90-min ELISA kit to measure Human alpha 1 Antitrypsin in serum and plasma. This SimpleStep ELISA® sensitivity is 0.02 ng/mL.

How the assay works

Human alpha 1 Antitrypsin SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Human IgM, human IgA and human IgE were prepared at 10 ng/mL and 250 ng/mL in Sample Diluent NS and assayed for cross reactivity. No cross-reactivity was observed for IgM or IgE at either concentration with a mean OD deviation from background of -0.01. No cross-reactivity was observed for IgA at 10 ng/mL and only 0.4% cross-reactivity at 250 ng/mL of IgA.
Purified human IgG was assayed at 5 ng/mL in the presence and absence of 250 ng/mL of human IgM, human IgA and human IgE to determine interference. After background subtraction, recovery of human IgG was observed at a mean of 98% with a standard deviation of 0.06.

Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) ab189579 protocol summary:

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm

How other researchers have used Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) ab189579

Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) ab189579 has been used in publications to study bioengineered liver grafts for treating acute liver failure ⁽¹⁾, alpha 1 antitrypsin for predicting acute kidney injury after cardiopulmonary bypass ⁽²⁾, and cystatin C correlation with alpha-1 antitrypsin in asthma⁽³⁾.

References:

⁽¹⁾Jeff J Ross et al. 2022 PMID: 37892931,
⁽²⁾Jun Ai et al. 2019 PMID: 32030221,
⁽³⁾Mohamad Sadikin et al. 2023 PMID: 38094096

Save your precious samples

Our 384-well Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) only requires a maxmum of 12.5µL

Related and recommended products

Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) ab189579 is commonly used to study liver function and inflammation.

Other kits for measuring liver function and inflammation include:

- Human Albumin ELISA kit Human Albumin ELISA Kit ab179887
- Human ALT ELISA kit Human ALT ELISA Kit ab234578
- Human AST ELISA Kit Human AST ELISA Kit (Aspartate Aminotransferase) ab263881

alpha 1 Antitrypsin (SERPINA1) is a serine protease inhibitor found in a variety of biological fluids. While it can inhibit trypsin, chymotrypsin, and plasminogen activator, it's main target is elastase. Alpha 1 antitrypsin plays a critical role in the lung by inhibiting the activity of elastase, produced by neutrophils during inflammatory responses. The aberrant form plays a role in coagulation and insulin induced NO synthesis. Alpha 1 antitrypsin deficiency results in emphysema and may also result in liver disease.