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AB254502

Human alpha-2 antiplasmin ELISA Kit

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(1 Publication)

Human alpha-2 antiplasmin ELISA Kit is a single-wash 90-min Simplestep used to quantify Human alpha-2 antiplasmin with a sensitivity of 29.4 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

AAP, PLI, SERPINF2, Alpha-2-antiplasmin, Alpha-2-AP, Alpha-2-plasmin inhibitor, Serpin F2, Alpha-2-PI

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ELISA - Human alpha-2 antiplasmin ELISA Kit (AB254502)
  • ELISA

Supplier Data

ELISA - Human alpha-2 antiplasmin ELISA Kit (AB254502)
Sandwich ELISA - Human alpha-2 antiplasmin ELISA Kit (AB254502)
  • sELISA

Supplier Data

Sandwich ELISA - Human alpha-2 antiplasmin ELISA Kit (AB254502)

Interpolated concentrations of native alpha-2 antiplasmin in human cell culture supernatant samples.

The concentrations of alpha-2 antiplasmin were measured in duplicates, interpolated from the alpha-2 antiplasmin standard curves and corrected for sample dilution. Undiluted samples are as follows : HepG2 cell culture supernatant 1 : 50. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean alpha-2 antiplasmin concentration was determined to be 185.1 ng/mL in neat HepG2 cell culture supernatant.

Sandwich ELISA - Human alpha-2 antiplasmin ELISA Kit (AB254502)
  • sELISA

Supplier Data

Sandwich ELISA - Human alpha-2 antiplasmin ELISA Kit (AB254502)

Interpolated concentrations of native alpha-2 antiplasmin in human serum and plasma samples.

The concentrations of alpha-2 antiplasmin were measured in duplicates, interpolated from the alpha-2 antiplasmin standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 20,000, plasma (citrate) 1 : 20,000, plasma (EDTA) 1 : 20,000, and plasma (heparin) 1 : 20,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean alpha-2 antiplasmin concentration was determined to be 40.2 μg/mL in neat serum, 70.3 μg/mL in neat plasma (citrate), 59.4 μg/mL in neat plasma (EDTA), and 43.5 μg/mL in neat plasma (heparin).

Sandwich ELISA - Human alpha-2 antiplasmin ELISA Kit (AB254502)
  • sELISA

Supplier Data

Sandwich ELISA - Human alpha-2 antiplasmin ELISA Kit (AB254502)

Serum from ten individual healthy human male donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean alpha-2 antiplasmin concentration was determined to be 36 μg/mL with a range of 25 – 50 μg/mL.

Sandwich ELISA - Human alpha-2 antiplasmin ELISA Kit (AB254502)
  • sELISA

Supplier Data

Sandwich ELISA - Human alpha-2 antiplasmin ELISA Kit (AB254502)

Example of human alpha-2 antiplasmin standard curve in Sample Diluent NS.

The alpha-2 antiplasmin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 29.4 pg/mL

Range

62.5 - 4000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human alpha-2 antiplasmin ELISA Kit ab254502 is a rapid single-wash 90-min Sandwich ELISA to measure Human alpha-2 antiplasmin in cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 29.4 pg/mL.

How the assay works

Human alpha-2 antiplasmin SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human alpha-2 antiplasmin ELISA Kit ab254502 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-PEDF antibody [EPR21067-285] - BSA and Azide free (Capture) ab259608
- Anti-PEDF antibody [EPR21067-2] - BSA and Azide free (Detector) ab259608

Alpha-2-antiplasmin is a serine protease inhibitor. The mature form of alpha-2-antiplasmin is generated by cleaved at Pro-39 by both the prolyl endopeptidase FAP form and antiplasmin-cleaving enzyme FAP soluble form. The major targets of this inhibitor are plasmin and trypsin, but it also inactivates matriptase-3/TMPRSS7 and chymotrypsin.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.1" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "100 - 113 %", "average": "= 106" }, { "sample": "Serum", "range": "101 - 112 %", "average": "= 105" }, { "sample": "EDTA Plasma", "range": "92 - 111 %", "average": "= 101" }, { "sample": "Heparin Plasma", "range": "104 - 110 %", "average": "= 106" }, { "sample": "Citrate plasma", "range": "101 - 112 %", "average": "= 106" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha 2 Antiplasmin also known as A2AP or anti-alpha 2 plays an important role in inhibiting the fibrinolytic system by inactivating plasmin. Weighing around 70 kDa this glycoprotein is mainly expressed in the liver and circulates in plasma. Its alternative names include alpha-2-antiplasmin and anti-alpha 2 which highlight its blocking role against excess plasmin action.
Biological function summary

Alpha 2 Antiplasmin functions as the primary inhibitor of plasmin blocking unwanted fibrinolysis. It can form a complex with fibrin which enhances its efficiency in preventing premature fibrin clot dissolution. This regulation of plasmin activity ensures the stability of clots essential for proper wound healing and controlling bleeding.

Pathways

Alpha 2 Antiplasmin engages significantly with the fibrinolysis and coagulation pathways. Its action balances the fibrinolytic process which involves transforming plasminogen to plasmin. Related proteins like plasminogen activator inhibitor also regulate plasmin maintaining equilibrium between clot formation and degradation.

Alpha 2 Antiplasmin has implications in hemorrhagic conditions and thrombotic disorders. Its deficiency might lead to excessive bleeding due to unregulated plasmin activity whilst elevated levels could contribute to thrombotic problems causing harmful clot formation. The interplay with plasminogen and its activation is important in understanding its disease linkages.
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Product protocols

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Target data

Serine protease inhibitor. The major targets of this inhibitor are plasmin and trypsin, but it also inactivates matriptase-3/TMPRSS7 and chymotrypsin.
See full target information SERPINF2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

PloS one 17:e0275901 PubMed36260611

2022

A liver secretome gene signature-based approach for determining circulating biomarkers of NAFLD severity.

Applications

Unspecified application

Species

Unspecified reactive species

Christoffer A Hagemann,Christian Legart,Mathias B Møllerhøj,Martin R Madsen,Henrik H Hansen,Merete J Kønig,Frederik Helgstrand,Flemming P Hjørne,Anders Toxværd,Jill L Langhoff,Urd L Kielgast,Lise L Gluud,Helene Ægidius,Kristoffer T G Rigbolt,Tina Vilsbøll,Jacob Jelsing,Filip K Knop
View all publications
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websiteProtocolBooklet
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