Human alpha-2 antiplasmin ELISA Kit is a single-wash 90-min Simplestep used to quantify Human alpha-2 antiplasmin with a sensitivity of 29.4 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
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- PloS one 17:e02759012022A liver secretome gene signature-based approach for determining circulating biomarkers of NAFLD severity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChristoffer A Hagemann,Christian Legart,Mathias B Møllerhøj,Martin R Madsen,Henrik H Hansen,Merete J Kønig,Frederik Helgstrand,Flemming P Hjørne,Anders Toxværd,Jill L Langhoff,Urd L Kielgast,Lise L Gluud,Helene Ægidius,Kristoffer T G Rigbolt,Tina Vilsbøll,Jacob Jelsing,Filip K KnopPubMed 36260611
Key facts
- Detection method
- Colorimetric
- Sample types
- Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 62.5 - 4000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 29.4 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Serine protease inhibitor. The major targets of this inhibitor are plasmin and trypsin, but it also inactivates matriptase-3/TMPRSS7 and chymotrypsin.
Alternative names
AAP, PLI, SERPINF2, Alpha-2-antiplasmin, Alpha-2-AP, Alpha-2-plasmin inhibitor, Serpin F2, Alpha-2-PI
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Human alpha-2 antiplasmin ELISA Kit is a single-wash 90-min Simplestep used to quantify Human alpha-2 antiplasmin with a sensitivity of 29.4 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 62.5 - 4000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 29.4 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 8 | mean - | SD - | C.V. 6.1 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. 5 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 106 | Range 100 - 113 % |
Sample type Serum | Average % = 105 | Range 101 - 112 % |
Sample type EDTA Plasma | Average % = 101 | Range 92 - 111 % |
Sample type Heparin Plasma | Average % = 106 | Range 104 - 110 % |
Sample type Citrate plasma | Average % = 106 | Range 101 - 112 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human alpha-2 antiplasmin ELISA Kit (ab254502) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of alpha-2 antiplasmin protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human alpha-2 antiplasmin with 29.4 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Alpha-2-antiplasmin is a serine protease inhibitor. The mature form of alpha-2-antiplasmin is generated by cleaved at Pro-39 by both the prolyl endopeptidase FAP form and antiplasmin-cleaving enzyme FAP soluble form. The major targets of this inhibitor are plasmin and trypsin, but it also inactivates matriptase-3/TMPRSS7 and chymotrypsin.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Alpha 2 Antiplasmin also known as A2AP or anti-alpha 2 plays an important role in inhibiting the fibrinolytic system by inactivating plasmin. Weighing around 70 kDa this glycoprotein is mainly expressed in the liver and circulates in plasma. Its alternative names include alpha-2-antiplasmin and anti-alpha 2 which highlight its blocking role against excess plasmin action.
- Biological function summary
Alpha 2 Antiplasmin functions as the primary inhibitor of plasmin blocking unwanted fibrinolysis. It can form a complex with fibrin which enhances its efficiency in preventing premature fibrin clot dissolution. This regulation of plasmin activity ensures the stability of clots essential for proper wound healing and controlling bleeding.
- Pathways
Alpha 2 Antiplasmin engages significantly with the fibrinolysis and coagulation pathways. Its action balances the fibrinolytic process which involves transforming plasminogen to plasmin. Related proteins like plasminogen activator inhibitor also regulate plasmin maintaining equilibrium between clot formation and degradation.
- Associated diseases and disorders
Alpha 2 Antiplasmin has implications in hemorrhagic conditions and thrombotic disorders. Its deficiency might lead to excessive bleeding due to unregulated plasmin activity whilst elevated levels could contribute to thrombotic problems causing harmful clot formation. The interplay with plasminogen and its activation is important in understanding its disease linkages.
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4 product images
Sandwich ELISA - Human alpha-2 antiplasmin ELISA Kit (ab254502)
Example of human alpha-2 antiplasmin standard curve in Sample Diluent NS.
The alpha-2 antiplasmin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human alpha-2 antiplasmin ELISA Kit (ab254502)
Interpolated concentrations of native alpha-2 antiplasmin in human serum and plasma samples.
The concentrations of alpha-2 antiplasmin were measured in duplicates, interpolated from the alpha-2 antiplasmin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:20,000, plasma (citrate) 1:20,000, plasma (EDTA) 1:20,000, and plasma (heparin) 1:20,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean alpha-2 antiplasmin concentration was determined to be 40.2 μg/mL in neat serum, 70.3 μg/mL in neat plasma (citrate), 59.4 μg/mL in neat plasma (EDTA), and 43.5 μg/mL in neat plasma (heparin).
Sandwich ELISA - Human alpha-2 antiplasmin ELISA Kit (ab254502)
Interpolated concentrations of native alpha-2 antiplasmin in human cell culture supernatant samples.
The concentrations of alpha-2 antiplasmin were measured in duplicates, interpolated from the alpha-2 antiplasmin standard curves and corrected for sample dilution. Undiluted samples are as follows: HepG2 cell culture supernatant 1:50. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean alpha-2 antiplasmin concentration was determined to be 185.1 ng/mL in neat HepG2 cell culture supernatant.
Sandwich ELISA - Human alpha-2 antiplasmin ELISA Kit (ab254502)
Serum from ten individual healthy human male donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean alpha-2 antiplasmin concentration was determined to be 36 μg/mL with a range of 25 – 50 μg/mL.
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