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AB314343

Human Alpha 2 Antiplasmin ELISA Kit

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Human Alpha 2 Antiplasmin ELISA Kit is a Sandwich ELISA for the measurement of Human Alpha 2 Antiplasmin in Human in Cell Culture Media, Biofluids samples.

View Alternative Names

AAP, PLI, SERPINF2, Alpha-2-antiplasmin, Alpha-2-AP, Alpha-2-plasmin inhibitor, Serpin F2, Alpha-2-PI

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Sandwich ELISA - Human Alpha 2 Antiplasmin ELISA Kit (AB314343)
  • sELISA

Supplier Data

Sandwich ELISA - Human Alpha 2 Antiplasmin ELISA Kit (AB314343)

Example of standard curve using ab314343. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Plasma, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.12 ng/mL

Range

0.12 - 30 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Alpha 2 Antiplasmin ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Alpha 2 Antiplasmin in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human Alpha 2 Antiplasmin coated on a 96-well plate.
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Recovery

[ { "sample": "Serum", "range": "69 - 100 %", "average": "= 77.49" }, { "sample": "Plasma", "range": "68 - 150 %", "average": "= 93.27" }, { "sample": "Cell culture media", "range": "68 - 77 %", "average": "= 71.78" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab314343-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB314343-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human Alpha 2 Antiplasmin Detection Antibody", "number":"AB314343-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB314343-CMP07", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent (Item E2)", "number":"AB314343-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human Alpha 2 Antiplasmin Antibody-coated ELISA Plate", "number":"AB314343-CMP08", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB314343-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human Alpha 2 Antiplasmin Protein Standard", "number":"AB314343-CMP10", "productcode":"" }, { "size":"1 x 200 µL", "name":"450X HRP-Streptavidin", "number":"AB314343-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha 2 Antiplasmin also known as A2AP or anti-alpha 2 plays an important role in inhibiting the fibrinolytic system by inactivating plasmin. Weighing around 70 kDa this glycoprotein is mainly expressed in the liver and circulates in plasma. Its alternative names include alpha-2-antiplasmin and anti-alpha 2 which highlight its blocking role against excess plasmin action.
Biological function summary

Alpha 2 Antiplasmin functions as the primary inhibitor of plasmin blocking unwanted fibrinolysis. It can form a complex with fibrin which enhances its efficiency in preventing premature fibrin clot dissolution. This regulation of plasmin activity ensures the stability of clots essential for proper wound healing and controlling bleeding.

Pathways

Alpha 2 Antiplasmin engages significantly with the fibrinolysis and coagulation pathways. Its action balances the fibrinolytic process which involves transforming plasminogen to plasmin. Related proteins like plasminogen activator inhibitor also regulate plasmin maintaining equilibrium between clot formation and degradation.

Alpha 2 Antiplasmin has implications in hemorrhagic conditions and thrombotic disorders. Its deficiency might lead to excessive bleeding due to unregulated plasmin activity whilst elevated levels could contribute to thrombotic problems causing harmful clot formation. The interplay with plasminogen and its activation is important in understanding its disease linkages.
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Product protocols

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Target data

Serine protease inhibitor. The major targets of this inhibitor are plasmin and trypsin, but it also inactivates matriptase-3/TMPRSS7 and chymotrypsin.
See full target information SERPINF2
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websiteProtocolBooklet
en
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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