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AB108888

Human alpha 2 Macroglobulin ELISA Kit

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(8 Publications)

Human alpha 2 Macroglobulin ELISA Kit is a competitive ELISA designed to quantify Human alpha 2 Macroglobulin with a sensitivity of 1.0 µg/mL

- Colorimetric competitive ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 1.25 - 40 µg/ml

View Alternative Names

CPAMD5, FWP007, A2M, Alpha-2-macroglobulin, Alpha-2-M, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5

2 Images
Competitive ELISA - Human alpha 2 Macroglobulin ELISA Kit (AB108888)
  • cELISA

Supplier Data

Competitive ELISA - Human alpha 2 Macroglobulin ELISA Kit (AB108888)

Standard curve : mean of duplicates (+/- SD) with background reads subtracted

Competitive ELISA - Human alpha 2 Macroglobulin ELISA Kit (AB108888)
  • cELISA

Unknown

Competitive ELISA - Human alpha 2 Macroglobulin ELISA Kit (AB108888)

Alpha 2 Macroglobulin measured in biological fluids and cell culture supernatant showing quantity (ug) per mL of tested sample. Human serum diluted 400-1600 fold. Human plasma diluted 80-320 fold. Other samples undiluted.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Serum

Reacts with

Human

Assay type

Competitive

Sensitivity

= 1 µg/mL

Range

1.25 - 40 µg/mL

Assay time

3h

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "cELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's alpha 2 Macroglobulin Human in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of alpha 2 Macroglobulin in plasma and serum.

An alpha 2 Macroglobulin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated alpha 2 Macroglobulin is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of alpha 2 Macroglobulin captured in plate.

The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.2" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab108888-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Vial", "name":"1X Biotinylated alpha 2 Macroglobulin (Lyophilized)", "number":"AB108888-CMP01", "productcode":"" }, { "size":"1 x 30 mL", "name":"20X Wash Buffer Concentrate", "number":"AB108888-CMP06", "productcode":"" }, { "size":"1 x 30 mL", "name":"10X Diluent M Concentrate", "number":"AB108888-CMP02", "productcode":"" }, { "size":"1 x 7 mL", "name":"Chromogen Substrate", "number":"AB108888-CMP05", "productcode":"" }, { "size":"1 x 1 Vial", "name":"alpha 2 Macroglobulin Standard", "number":"AB108888-CMP03", "productcode":"" }, { "size":"1 x 80 µL", "name":"100X Streptavidin-Peroxidase Conjugate", "number":"AB108888-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"alpha 2 Macroglobulin Microplate (12 x 8 well strips)", "number":"AB108888-CMP04", "productcode":"" }, { "size":"3 x 1 Unit", "name":"Sealing Tapes", "number":"AB108888-CMP07", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Solution", "number":"AB108888-CMP08", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha-2-macroglobulin also known as alpha-2-M and A2M is a large glycoprotein with a molecular mass of approximately 725 kDa. It is expressed in the liver and is found in high concentrations in the blood plasma. This protein functions mechanically as a broad-spectrum protease inhibitor. It can trap proteases within its structure preventing them from accessing substrates. Additionally alpha-2-macroglobulin can bind to cytokines and growth factors altering their availability and activities.
Biological function summary

Alpha-2-macroglobulin plays an important role in maintaining homeostasis by regulating protease activities and modulating immune system reactions. It does not form a permanent complex but can transiently associate with the proteases and various molecules mentioned. By interacting with proteases it controls processes like coagulation and fibrinolysis and participates in the clearance of proteinases and growth factors from the circulation. It influences cell signaling pathways and inflammatory responses highlighting its significance in maintaining balance within the body.

Pathways

Alpha-2-macroglobulin is an important component in the immune response and coagulation pathways. In the complement system it binds with complement components to regulate their activities. It associates with proteins such as plasmin and thrombin which are important for blood clotting and fibrinolysis. These relationships emphasize the protein's involvement in both protective mechanisms against pathogens and the regulation of blood coagulation and breakdown connecting the immune and hemostatic systems.

Alpha-2-macroglobulin has been linked to Alzheimer's disease and liver diseases. In Alzheimer's altered levels of this protein might affect the amyloid-beta peptide clearance due to its protease inhibition and binding properties. It associates with proteins like lipoprotein receptor-related protein-1 (LRP1) influencing amyloid-beta metabolism. In liver diseases its levels can indicate liver dysfunction or inflammation given its synthesis in the liver and can relate to proteins involved in inflammation such as cytokines. Its modulation of protease activity makes it a critical target in understanding these disorders.
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Product protocols

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Target data

Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region, a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.
See full target information A2M
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:4523 PubMed39915642

2025

Increasing the concentration of plasma molecules improves the biological activity of platelet-rich plasma for tissue regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Mikel Sánchez,Jon Mercader Ruiz,Daniel Marijuán Pinel,Pello Sánchez,Nicolás Fiz,Jorge Guadilla,Juan Azofra,Maider Beitia,Diego Delgado

Translational psychiatry 12:377 PubMed36085284

2022

Transdiagnostic inflammatory subgroups among psychiatric disorders and their relevance to role functioning: a nested case-control study of the ALSPAC cohort.

Applications

Unspecified application

Species

Unspecified reactive species

Jonah F Byrne,Colm Healy,David Mongan,Subash Raj Susai,Stan Zammit,Melanie Fӧcking,Mary Cannon,David R Cotter

Neuropsychiatric disease and treatment 15:2853-2867 PubMed31632033

2019

Cerebrospinal Fluid Proteomics For Identification Of α2-Macroglobulin As A Potential Biomarker To Monitor Pharmacological Therapeutic Efficacy In Dopamine Dictated Disease States Of Parkinson's Disease And Schizophrenia.

Applications

Unspecified application

Species

Unspecified reactive species

Ashish Kumar Gupta,Ruchika Pokhriyal,Mohd Imran Khan,Domada Ratna Kumar,Rishab Gupta,Rakesh Kumar Chadda,Rashmi Ramachandran,Vinay Goyal,Manjari Tripathi,Gururao Hariprasad

The archives of bone and joint surgery 6:219-224 PubMed29911139

2018

High Serum Alpha-2-Macroglobulin Level in Patients with Osteonecrosis of the Femoral Head.

Applications

Unspecified application

Species

Unspecified reactive species

Zari Naderi Ghale-Noie,Mohammad Hassani,Amir R Kachooei,Mohammad A Kerachian

Journal of translational medicine 15:258 PubMed29258604

2017

Water T as an early, global and practical biomarker for metabolic syndrome: an observational cross-sectional study.

Applications

Unspecified application

Species

Unspecified reactive species

Michelle D Robinson,Ina Mishra,Sneha Deodhar,Vipulkumar Patel,Katrina V Gordon,Raul Vintimilla,Kim Brown,Leigh Johnson,Sid O'Bryant,David P Cistola

Molecular & cellular proteomics : MCP 16:924-935 PubMed28336724

2017

Quantitative Age-specific Variability of Plasma Proteins in Healthy Neonates, Children and Adults.

Applications

Unspecified application

Species

Unspecified reactive species

Stefan Bjelosevic,Dana Pascovici,Hui Ping,Vasiliki Karlaftis,Thiri Zaw,Xiaomin Song,Mark P Molloy,Paul Monagle,Vera Ignjatovic

Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology 46:813-24 PubMed27228572

2016

A proteomics analysis reveals that A2M might be regulated by STAT3 in persistent allergic rhinitis.

Applications

Unspecified application

Species

Unspecified reactive species

X Chen,Z H Xie,Y X Lv,Q P Tang,H Zhang,J Y Zhang,B Wu,W H Jiang

Experimental gerontology 58:154-8 PubMed25106097

2014

Protein molecular forms of insulin-like growth factor binding protein-2 change with aging.

Applications

Unspecified application

Species

Unspecified reactive species

Miloš Sunderić,Nevena Mihailović,Olgica Nedić
View all publications
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