AB322431

Human alpha 2 Macroglobulin ELISA Kit

Recombinant
SimpleStep
What is this?

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Human alpha 2 Macroglobulin ELISA Kit is a single-wash 90-min Simplestep used to quantify Human alpha 2 Macroglobulin with a sensitivity of 22 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Validated on a number of sample types including cerebrospinal fluid (CSF)

View Alternative Names

CPAMD5, FWP007, A2M, Alpha-2-macroglobulin, Alpha-2-M, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5

8 Images

Sandwich ELISA - Human alpha 2 Macroglobulin ELISA Kit (AB322431)

sELISA

Supplier Data

Sandwich ELISA - Human alpha 2 Macroglobulin ELISA Kit (AB322431)

Interpolated concentration of native alpha 2 Macroglobulin was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1 : 500,000 diluted serum. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Human alpha 2 Macroglobulin ELISA Kit (AB322431)

Interpolated concentrations of human alpha 2 Macroglobulin in serum, plasma (citrate), plasma (EDTA), and plasma (heparin).

sELISA

Supplier Data

Sandwich ELISA - Human alpha 2 Macroglobulin ELISA Kit (AB322431)

Interpolated concentration of native alpha 2 Macroglobulin was measured in duplicate at different sample concentrations. Undiluted samples are 50% urine. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Human alpha 2 Macroglobulin ELISA Kit (AB322431)

Serial dilutions of serum from eight individual healthy human female donors were measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean alpha 2 Macroglobulin concentration was determined to be 1,781 µg/mL with a range of 1,211 – 2,258 µg/mL.

sELISA

Supplier Data

Sandwich ELISA - Human alpha 2 Macroglobulin ELISA Kit (AB322431)

Serial dilutions of human, rat, mouse, monkey, and cow serum samples were measured in duplicate and interpolated from the human standard curve. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Human alpha 2 Macroglobulin ELISA Kit (AB322431)

Interpolated concentrations of human alpha 2 Macroglobulin in serum, plasma (citrate), plasma (EDTA), and plasma (heparin).

sELISA

Supplier Data

Sandwich ELISA - Human alpha 2 Macroglobulin ELISA Kit (AB322431)

Example of human alpha 2 Macroglobulin standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human alpha 2 Macroglobulin ELISA Kit (AB322431)

Example of human alpha 2 Macroglobulin standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture supernatant, Urine, Milk, Saliva, Cerebral Spinal Fluid

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 22 pg/mL

Range

87.5 - 5600 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human alpha 2 Macroglobulin SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of alpha 2 Macroglobulin protein in Serum, Cit Plasma, EDTA Plasma, Hep Plasma, Cell culture supernatant, Urine, Saliva, and Milk, Cerebral Spinal Fluid. Quantitate Human alpha 2 Macroglobulin with 22 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
-Also available in a fully validated 384-well format.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.8" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.5" } ]

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Recovery

[ { "sample": "Serum", "range": "107 - 116 %", "average": "= 112" }, { "sample": "Citrate plasma", "range": "98 - 104 %", "average": "= 101" }, { "sample": "EDTA Plasma", "range": "100 - 111 %", "average": "= 107" }, { "sample": "Heparin Plasma", "range": "100 - 108 %", "average": "= 104" }, { "sample": "Cell culture supernatant", "range": "107 - 118 %", "average": "= 111" }, { "sample": "Urine", "range": "101 - 110 %", "average": "= 105" }, { "sample": "Saliva", "range": "102 - 114 %", "average": "= 108" }, { "sample": "Milk", "range": "100 - 112 %", "average": "= 105" }, { "sample": "Cerebral Spinal Fluid", "range": "98 - 109 %", "average": "= 102" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region, a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.

See full target information A2M

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websiteProtocolBooklet

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Product promise

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