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AB193765

Human alpha Fetoprotein ELISA Kit

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(11 Publications)

Human alpha Fetoprotein ELISA Kit is a single-wash 90-min Simplestep used to quantify Human alpha Fetoprotein with a sensitivity of 44.8 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 5 citations

View Alternative Names

HPAFP, AFP, Alpha-fetoprotein, Alpha-1-fetoprotein, Alpha-fetoglobulin

6 Images
ELISA - Human alpha Fetoprotein ELISA Kit (AB193765)
  • ELISA

Supplier Data

ELISA - Human alpha Fetoprotein ELISA Kit (AB193765)
Sandwich ELISA - Human alpha Fetoprotein ELISA Kit (AB193765)
  • sELISA

Supplier Data

Sandwich ELISA - Human alpha Fetoprotein ELISA Kit (AB193765)

Serum from ten individual healthy human male donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean AFP concentration was determined to be 678.4 pg/mL with a range of 133.9 – 2,257 pg/mL.

Sandwich ELISA - Human alpha Fetoprotein ELISA Kit (AB193765)
  • sELISA

Supplier Data

Sandwich ELISA - Human alpha Fetoprotein ELISA Kit (AB193765)

Interpolated concentrations of native AFP in pregnant human serum and HepG2 cell culture supernatant samples.

The concentrations of AFP were measured in duplicates, interpolated from the AFP standard curves and corrected for sample dilution. Undiluted samples are as follows : pregnant human serum 3% and HepG2 cell culture supernatant 1 : 666. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean AFP concentration was determined to be 221.8 ng/mL in neat pregnant human serum and 6,165 ng/mL in neat HepG2 cell culture supernatant.

Sandwich ELISA - Human alpha Fetoprotein ELISA Kit (AB193765)
  • sELISA

Supplier Data

Sandwich ELISA - Human alpha Fetoprotein ELISA Kit (AB193765)

Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean AFP concentration in nine detectable donor samples was determined to be 550.7 pg/mL with a range of 247.9-816.1 pg/mL. Donor #5 was not detectable.

Sandwich ELISA - Human alpha Fetoprotein ELISA Kit (AB193765)
  • sELISA

Supplier Data

Sandwich ELISA - Human alpha Fetoprotein ELISA Kit (AB193765)

Example of human AFP standard curve in Sample Diluent 75BS.

The AFP standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human alpha Fetoprotein ELISA Kit (AB193765)
  • sELISA

Supplier Data

Sandwich ELISA - Human alpha Fetoprotein ELISA Kit (AB193765)

Human AFP standard curve comparison.

Standard Curve comparison between human AFP SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

Key facts

Detection method

Colorimetric

Sample types

Urine, Cell culture media, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 44.8 pg/mL

Range

156.25 - 10000 pg/mL

Assay time

1h 30m

Assay Platform

Microplate (12 x 8 well strips)

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Primary Antibodies

AB46799

Anti-alpha 1 Fetoprotein antibody

Western blot - Anti-alpha 1 Fetoprotein antibody (AB46799)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human alpha Fetoprotein ELISA Kit ab193765 is a rapid single-wash 90-min Sandwich ELISA to measure Human alpha Fetoprotein in cell culture media, cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, serum, urine. This SimpleStep sensitivity is 44.8 pg/mL.

How the assay works

Human alpha Fetoprotein SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human alpha Fetoprotein ELISA Kit ab193765 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-alpha 1 Fetoprotein antibody [EPR20365-101] - BSA and Azide free (Capture) ab252632
- Anti-alpha 1 Fetoprotein antibody [EPR20364-26] - BSA and Azide free (Detector) ab252632

Alpha-fetoprotein (AFP) is a secreted single chain glycoprotein expressed normally during fetal development as well as by liver pathologies, including cancer. It is a member of the albuminoid superfamily and may act as a carrier protein. Pregnant women may have elevated alpha-fetoprotein levels derived from fetal circulation and male and female adults have low levels of circulating alpha-fetoprotein.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.5" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "101 - 113 %", "average": "= 103" }, { "sample": "Serum", "range": "88 - 96 %", "average": "= 91" }, { "sample": "EDTA Plasma", "range": "98 - 102 %", "average": "= 100" }, { "sample": "Urine", "range": "116 - 121 %", "average": "= 119" }, { "sample": "Cell culture media", "range": "91 - 98 %", "average": "= 94" }, { "sample": "Heparin Plasma", "range": "94 - 97 %", "average": "= 96" }, { "sample": "Citrate plasma", "range": "89 - 89 %", "average": "= 89" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha-fetoprotein also known as alpha 1-fetoprotein or AFP is a glycoprotein composed of a single polypeptide chain with a molecular mass of approximately 69 kDa. AFP is primarily expressed in the fetal liver yolk sac and gastrointestinal tract during embryonic development. In adults the expression of AFP is limited and occurs mainly in the liver. The protein belongs to the albuminoid gene family and shares structural similarity with serum albumin contributing to its role in fetal serum transport.
Biological function summary

Alpha-fetoprotein functions as a fetal plasma protein with roles similar to serum albumin including the binding and transport of ligands such as bilirubin and fatty acids. It does not form part of any larger protein complex but is significant in modulating fetal immune tolerance. In the fetal environment AFP helps protect cells from maternal immune attacks by binding to estrogens and other growth-modulating factors. Its function during pregnancy is critical for maintaining homeostasis and ensuring the fetus's viability.

Pathways

Alpha 1-fetoprotein plays a role in several important biological pathways related to cellular growth and immune response modulation. One such pathway involves regulation of cell proliferation and differentiation in the developing liver. AFP interacts with cellular receptors that influence these processes. Additionally it plays a part in immune pathways interacting with elements that control immune overlooking to fetal antigens hence being linked to both hepatic and immune system proteins like albumin and estrogen receptors.

High levels of alpha-fetoprotein are linked to hepatocellular carcinoma and germ cell tumors such as yolk sac tumor. AFP serves as a tumor marker in these conditions aiding in both diagnosis and monitoring treatment response. In liver cancer AFP levels often correlate with tumor burden while in germ cell tumors it connects to proteins like beta-human chorionic gonadotropin (beta-hCG). Both markers are used together in clinical settings to provide insights into tumor progression and therapeutic efficacy.
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Product protocols

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Target data

Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.
See full target information AFP
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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Journal of the Egyptian National Cancer Institute 36:32 PubMed39428452

2024

Association of IL-6 G-174C (rs1800795) variant with the susceptibility to hepatocellular carcinoma in patients with chronic hepatitis.

Applications

Unspecified application

Species

Unspecified reactive species

Eman H Abuelnadar,Lamiaa M Ramadan,Hanaa Elsayed Shahin,Saleha Y M Alakilli,Eman Wahsh,Nanis S El-Beltagy,Eman T Salem,Abdelrahman S Hatata,Afaf M El-Said,Maha Alhelf

Oncogenesis 10:69 PubMed34650031

2021

GP73-mediated secretion of AFP and GP73 promotes proliferation and metastasis of hepatocellular carcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yujuan Liu,Jiyin Wang,Ruixiang Yang,Yuning Cheng,Yue Zhou,Hui Li,Wei Jiang,Xiaowei Zhang

Environmental science and pollution research international 28:37661-37671 PubMed33721166

2021

Doxorubicin, L-arginine, or their combination as a prophylactic agent against hepatic carcinoma in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Eman A Al-Shahari,Amira Ragab El Barky,Tarek M Mohamed,Abeer A Alm-Eldeen

Metallomics : integrated biometal science 12:249-258 PubMed31815268

2019

Comprehensive and comparative exploration of the Atp7b mouse plasma proteome.

Applications

Unspecified application

Species

Unspecified reactive species

Maud Lacombe,Michel Jaquinod,Lucid Belmudes,Yohann Couté,Claire Ramus,Florence Combes,Thomas Burger,Elisabeth Mintz,Justine Barthelon,Vincent Leroy,Aurélia Poujois,Alain Lachaux,France Woimant,Virginie Brun

Analytical chemistry 91:12055-12062 PubMed31436433

2019

H-Based Electrochemical Biosensor with Pd Nanowires@ZIF-67 Molecular Sieve Bilayered Sensing Interface for Immunoassay.

Applications

Unspecified application

Species

Unspecified reactive species

Shuzhen Lv,Kangyao Zhang,Ling Zhu,Dianping Tang,Reinhard Niessner,Dietmar Knopp

The Analyst 144:4858-4864 PubMed31294738

2019

Prussian blue nanoparticle-labeled aptasensing platform on graphene oxide for voltammetric detection of α-fetoprotein in hepatocellular carcinoma with target recycling.

Applications

Unspecified application

Species

Unspecified reactive species

Bei Zhang,Hao Ding,Qi Chen,Ting Wang,Kunhe Zhang

Scientific reports 9:8920 PubMed31222080

2019

Generation of fully functional hepatocyte-like organoids from human induced pluripotent stem cells mixed with Endothelial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Giuseppe Pettinato,Sylvain Lehoux,Rajesh Ramanathan,Mohamed M Salem,Li-Xia He,Oluwatoyosi Muse,Robert Flaumenhaft,Melissa T Thompson,Emily A Rouse,Richard D Cummings,Xuejun Wen,Robert A Fisher

Cancer management and research 11:1349-1362 PubMed30809100

2019

Activated Wnt signaling promotes growth and progression of AFP-producing gastric cancer in preclinical models.

Applications

Unspecified application

Species

Unspecified reactive species

Dongshao Chen,Xiaoting Lin,Cheng Zhang,Guo An,Zhongwu Li,Bin Dong,Lin Shen,Jing Gao,Xiaotian Zhang

PloS one 13:e0198552 PubMed29879168

2018

Extracellular mRNA detected by molecular beacons in tethered lipoplex nanoparticles for diagnosis of human hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xinmei Wang,Kwang Joo Kwak,Zhaogang Yang,Aili Zhang,Xiaoli Zhang,Rachael Sullivan,Dan Lin,Robert L Lee,Carlos Castro,Kalpana Ghoshal,Carl Schmidt,L James Lee

Scientific reports 6:32888 PubMed27616299

2016

Scalable Differentiation of Human iPSCs in a Multicellular Spheroid-based 3D Culture into Hepatocyte-like Cells through Direct Wnt/β-catenin Pathway Inhibition.

Applications

sELISA

Species

Human

Giuseppe Pettinato,Rajesh Ramanathan,Robert A Fisher,Martin J Mangino,Ning Zhang,Xuejun Wen
View all publications
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