AB240678

Human alpha SMA ELISA Kit

Recombinant
SimpleStep
What is this?

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(9 Publications)

Human alpha SMA ELISA Kit is a single-wash 90-min Simplestep used to quantify Human alpha SMA with a sensitivity of 0.2 ng/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 5 citations

View Alternative Names

ACTSA, ACTVS, GIG46, ACTA2, Alpha-actin-2, Cell growth-inhibiting gene 46 protein

6 Images

ELISA - Human alpha SMA ELISA Kit (AB240678)

ELISA

Supplier Data

ELISA - Human alpha SMA ELISA Kit (AB240678)

sELISA

Lab

Sandwich ELISA - Human alpha SMA ELISA Kit (AB240678)

Interpolated concentration of native Human alpha SMA was measured in duplicate at different sample concentrations. Undiluted samples are 0.5 µg/mL human colon tissue extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

sELISA

Lab

Sandwich ELISA - Human alpha SMA ELISA Kit (AB240678)

Example of species Human alpha SMA standard curve in 96-well vs. 384-well plate in 1X Cell Extraction Buffer PTR. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Lab

Sandwich ELISA - Human alpha SMA ELISA Kit (AB240678)

Interpolated concentration of native Human alpha SMA was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 0.5 µg/mL human colon tissue extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

sELISA

Lab

Sandwich ELISA - Human alpha SMA ELISA Kit (AB240678)

Example of species Human alpha SMA standard curve in 1X Cell Extraction Buffer PTR. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human alpha SMA ELISA Kit (AB240678)

Representative image of Human alpha SMA ELISA Kit (ab240678)

Components shown from left to right :

- 10X Wash Buffer PT (ab206977)

- 10X Human alpha SMA Capture Antibody

- 10X Human alpha SMA Detector Antibody

- Human alpha SMA Lyophilised Purified Protein (2 vials)

- 50X Cell Extraction Enhancer Solution (ab193971)

- Sample diluent NS

- TMB solution

- Stop Solution

- 5X Cell Extraction Buffer PTR (ab193970)

- Antibody diluent 5BI

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

Key facts

Detection method

Colorimetric

Sample types

Tissue Extracts

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.2 ng/mL

Range

0.7 - 45 ng/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human alpha SMA ELISA Kit ab240678 is a rapid single-wash 90-min Sandwich ELISA to measure Human alpha SMA in cell culture extracts, tissue extracts. This SimpleStep sensitivity is 21.5 pg/mL.

How the assay works

Human alpha SMA SimpleStep ELISA^{^®}employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^{^®} plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^{^®} protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human alpha SMA ELISA Kit ab240678 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Save your precious samples

Our 384-well Human alpha SMA ELISA Kit only requires a maximum of 12.5µL sample

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-alpha smooth muscle Actin antibody [EPR20062-47] - BSA and Azide free (Capture) ab244818
- Anti-alpha smooth muscle Actin antibody [EPR20062-77] - BSA and Azide free (Detector) ab245091

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Colon", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.8" }, { "reproducibilityType": "Intra", "sample": "Colon", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.2" } ]

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Recovery

[ { "sample": "Tissue Extracts", "range": "89 - 112 %", "average": "= 101" } ]

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What's included?

{ "values": { "1x384Tests": { "sellingSize": "1 x 384 Tests", "publicAssetCode":"ab240678-1x384Tests", "assetComponentDetails": [ { "size":"2 x 12 mL", "name":"Stop Solution", "number":"AB240678-CMP07" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB240678-CMP12" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB240678-CMP05" }, { "size":"1 x 600 µL", "name":"10X Human alpha SMA Capture Antibody", "number":"AB240678-CMP02" }, { "size":"2 x 1 Vial", "name":"Human alpha SMA Lyophilized Recombinant Protein", "number":"AB240678-CMP01" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB240678-CMP11" }, { "size":"1 x 50 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB240678-CMP10" }, { "size":"1 x 600 µL", "name":"10X Human alpha SMA Detector Antibody", "number":"AB240678-CMP03" }, { "size":"2 x 12 mL", "name":"TMB Development Solution", "number":"AB240678-CMP08" }, { "size":"1 x 1 Unit", "name":"384 well CaptSure™ microplates", "number":"AB240678-CMP14" }, { "size":"6 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB240678-CMP09" } ] }, "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab240678-10x96Tests", "assetComponentDetails": [ { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB240678-CMP12" }, { "size":"10 x 600 µL", "name":"10X Human alpha SMA Capture Antibody", "number":"AB240678-CMP02" }, { "size":"10 x 2 Vial", "name":"Human alpha SMA Lyophilized Recombinant Protein", "number":"AB240678-CMP01" }, { "size":"10 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB240678-CMP05" }, { "size":"1 x 120 mL", "name":"TMB Development Solution", "number":"AB240678-CMP08" }, { "size":"10 x 1 Unit", "name":"Plate Seals", "number":"AB240678-CMP11" }, { "size":"10 x 600 µL", "name":"10X Human alpha SMA Detector Antibody", "number":"AB240678-CMP03" }, { "size":"2 x 50 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB240678-CMP10" }, { "size":"10 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB240678-CMP09" }, { "size":"1 x 120 mL", "name":"Stop Solution", "number":"AB240678-CMP07" }, { "size":"10 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB240678-CMP06" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab240678-1x96Tests", "assetComponentDetails": [ { "size":"1 x 600 µL", "name":"10X Human alpha SMA Capture Antibody", "number":"AB240678-CMP02", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB240678-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB240678-CMP11", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human alpha SMA Detector Antibody", "number":"AB240678-CMP03", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB240678-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human alpha SMA Lyophilized Recombinant Protein", "number":"AB240678-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB240678-CMP08", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB240678-CMP12", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB240678-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB240678-CMP06", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB240678-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB240678-CMP13", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha smooth muscle actin (?-SMA) also known as ACTA2 is an actin isoform with a specific role in the contractile function of smooth muscle cells. The molecular weight of alpha-SMA is approximately 42 kDa. This protein is expressed widely in vascular smooth muscle cells the peritoneal lining and myofibroblasts. It often serves as a marker for these cell types. The expression of alpha-SMA is critical for the mechanical attributes of cells contributing to the rigidity and contractility of tissues where it is present.

Biological function summary

Alpha smooth muscle actin aids in maintaining the structural integrity of tissues by forming part of the actin cytoskeleton an important element in cellular support. It exists in high concentration in stress fibers contributing to cellular movements and shape maintenance. These actions are essential in various dynamic cellular processes such as cell migration and adhesion. Alpha smooth muscle actin does not typically form complexes but it associates with other components of the actin cytoskeleton to ensure cell stability and function.

Pathways

Alpha smooth muscle actin functions significantly within the TGF-beta signaling pathway which influences cell proliferation differentiation and apoptosis. It interacts closely with proteins such as myosin to facilitate cellular contractility and motility. Additionally alpha-SMA plays a part in the RhoA/Rho kinase (ROCK) pathway connecting with regulators of actin filament organization. These pathways are essential for modulation of smooth muscle contraction and actin filament assembly contributing to vascular development and wound healing.

Alpha smooth muscle actin is importantly involved in fibrotic diseases and vascular diseases like arteriosclerosis. Increased expression of alpha-SMA is often observed in fibrotic tissues contributing to pathogenesis due to excessive extracellular matrix deposition. In the context of vascular disorders overexpression of alpha-SMA can lead to abnormal vascular remodeling often seen in diseases like hypertension. Interactions with proteins such as fibronectin and collagens facilitate these pathological changes highlighting the importance of alpha-SMA in disease development.

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Product protocols

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Target data

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

See full target information ACTA2

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Journal of cosmetic dermatology 24:e70177 PubMed40214031

2025

ALKBH5 Inhibits YTHDF2-m6A-Mediated Degradation of RCN1 mRNA to Promote Keloid Formation by Activating IRE1α-XBP1-Mediated ER Stress.

Applications

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Species

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Min Shi,Lu Zhang,Fangfang Bi,Zhuo Zhou

Biomedicines 11: PubMed37760792

2023

Is Spheroid a Relevant Model to Address Fibrogenesis in Keloid Research?

Applications

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Species

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Zélie Dirand,Marion Tissot,Brice Chatelain,Céline Viennet,Gwenaël Rolin

Journal of cellular and molecular medicine 27:1867-1879 PubMed37259630

2023

Anti-fibrotic effect of ciglitazone in HRV-induced airway remodelling cell model.

Applications

Unspecified application

Species

Unspecified reactive species

Joanna Wieczfinska,Rafal Pawliczak

Advanced functional materials 33: PubMed37601745

2023

Simulation of Cortical and Cancellous Bone to Accelerate Tissue Regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Zhihai Fan,Hongxiang Liu,Zhaozhao Ding,Liying Xiao,Qiang Lu,David L Kaplan

Materials today. Bio 15:100283 PubMed35634170

2022

Anisotropic silk nanofiber layers as regulators of angiogenesis for optimized bone regeneration.

Applications

Unspecified application

Species

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Zhihai Fan,Hongxiang Liu,Shilei Shi,Zhaozhao Ding,Zhen Zhang,Qiang Lu,David L Kaplan

Experimental and therapeutic medicine 22:1349 PubMed34659495

2021

YAP1 promotes high glucose-induced inflammation and extracellular matrix deposition in glomerular mesangial cells by modulating NF-κB/JMJD3 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Wang,Jinmei Xu,Zhifeng Cheng

Journal of inflammation research 14:3217-3229 PubMed34285545

2021

Mesenchymal Stem Cell-Derived Exosomes Induced by IL-1β Attenuate Urethral Stricture Through Let-7c/PAK1/NF-κB-Regulated Macrophage M2 Polarization.

Applications

Unspecified application

Species

Unspecified reactive species

Ye-Hui Chen,Ru-Nan Dong,Jian Hou,Ting-Ting Lin,Shao-Hao Chen,Hang Chen,Jun-Ming Zhu,Jia-Yin Chen,Zhi-Bin Ke,Fei Lin,Xue-Yi Xue,Yong Wei,Ning Xu

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 135:111182 PubMed33433355

2021

Halofuginone regulates keloid fibroblast fibrotic response to TGF-β induction.

Applications

Unspecified application

Species

Unspecified reactive species

Pierre Marty,Brice Chatelain,Thomas Lihoreau,Marion Tissot,Zélie Dirand,Philippe Humbert,Clémence Senez,Eleonora Secomandi,Ciro Isidoro,Gwenaël Rolin

Drug design, development and therapy 14:3547-3557 PubMed32921988

2020

LncRNA PVT1 Suppresses the Progression of Renal Fibrosis via Inactivation of TGF-β Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Lu Cao,Peng Qin,Jianjiang Zhang,Huiju Qiao,Peipei Shi,Huali Huo

View all publications

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websiteProtocolBooklet

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Product promise

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Human alpha SMA ELISA Kit

ELISA - Human alpha SMA ELISA Kit (AB240678)

Sandwich ELISA - Human alpha SMA ELISA Kit (AB240678)

Sandwich ELISA - Human alpha SMA ELISA Kit (AB240678)

Sandwich ELISA - Human alpha SMA ELISA Kit (AB240678)

Sandwich ELISA - Human alpha SMA ELISA Kit (AB240678)

Sandwich ELISA - Human alpha SMA ELISA Kit (AB240678)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (9)

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Product promise