Human Alpha-synuclein ELISA Kit

Human Alpha-synuclein ELISA Kit (ab260052) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Alpha-synuclein protein in cell culture media, cell lysate, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Alpha-synuclein with 504 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

ASSAY SPECIFICITY

This kit recognizes both native and recombinant human Alpha-synuclein protein in serum, plasma, and cell culture supernatant, cell and tissue extract samples only.

Urine, saliva, and cerebrospinal fluid samples have not been tested with this kit.

CROSS REACTIVITY

Recombinant Tau 441, UCHL-1, beta-tubulin, and Gamma-synuclein were prepared at 7,000 pg/mL and assayed for cross reactivity. No cross-reactivity was observed.

INTERFERENCE

Recombinant Tau 441, UCHL-1, and beta tubulin were prepared at 4 ng/mL and tested for interference. No interference was observed.

SPECIES REACTIVITY

This kit recognizes human Alpha-synuclein protein.

Other species reactivity was determined by measuring 50% serum samples of various species, interpolating the protein concentrations from the human standard curve, and expressing the interpolated concentrations as a percentage of the protein concentration in human serum assayed at the same dilution.

This kit was found to react with rhesus macaque serum.

Reactivity < 3% was determined for the following species:

• Mouse


• Rat


• Cow

Other species reactivity not determined.

Alpha-synuclein is a 140- amino acid protein encoded by the SNCA gene, primarily found in neural tissue. It is a member of the synuclein family of proteins, including β-Synuclein and γ-Synuclein. Alpha-synuclein has been found concentrated in the presynaptic nerve terminals of neurons and has been found in the nucleus of neurons. Alpha-synuclein is a major component of Lewy Bodies. This protein appears to play a role in dopamine metabolism and vesicle trafficking.

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