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AB260052

Human Alpha-synuclein ELISA Kit

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(6 Publications)

Human Alpha-synuclein ELISA kit is a single-wash, 90-minute SimpleStep ELISA® used to quantify human alpha-synuclein (SNCa) with a sensitivity of 504 pg/mL. The assay employs a simple Mix-Wash-Read protocol with just one incubation and wash step.

- Colorimetric sandwich ELISA with a 450 nm readout; compatible with any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready carrier-free antibody pair
- KO validated for guaranteed specificity

View Alternative Names

NACP, PARK1, SNCA, Alpha-synuclein, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor

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ELISA - Human Alpha-synuclein ELISA Kit (AB260052)
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Supplier Data

ELISA - Human Alpha-synuclein ELISA Kit (AB260052)
Sandwich ELISA - Human Alpha-synuclein ELISA Kit (AB260052)
  • sELISA

Unknown

Sandwich ELISA - Human Alpha-synuclein ELISA Kit (AB260052)

Raw data is plotted for wild-type and alpha synuclein-knockout HAP1 cell extracts tested at 111 μg/mL.

Sandwich ELISA - Human Alpha-synuclein ELISA Kit (AB260052)
  • sELISA

Supplier Data

Sandwich ELISA - Human Alpha-synuclein ELISA Kit (AB260052)

Serum from eight individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Alpha-synuclein concentration was determined to be 7.8 ng/mL with a range of 3.0 – 8.9 ng/mL.

Sandwich ELISA - Human Alpha-synuclein ELISA Kit (AB260052)
  • sELISA

Supplier Data

Sandwich ELISA - Human Alpha-synuclein ELISA Kit (AB260052)

Human Alpha Synuclein rabmab remake comparison.

Standard Curve comparison between the original Human Alpha-synuclein SimpleStep ELISA kit (ab210973) and current Human Alpha-synuclein SimpleStep ELISA kit (ab260052).

Sandwich ELISA - Human Alpha-synuclein ELISA Kit (AB260052)
  • sELISA

Supplier Data

Sandwich ELISA - Human Alpha-synuclein ELISA Kit (AB260052)

Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.

Native Alpha-synuclein was measured in the following biological samples in a 1.5-fold dilution series. Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human Alpha-synuclein ELISA Kit (AB260052)
  • sELISA

Supplier Data

Sandwich ELISA - Human Alpha-synuclein ELISA Kit (AB260052)

Example of human Alpha-synuclein standard curve in 1X Cell Extraction Buffer PTR.

The Alpha-synuclein standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Alpha-synuclein ELISA Kit (AB260052)
  • sELISA

Supplier Data

Sandwich ELISA - Human Alpha-synuclein ELISA Kit (AB260052)

Interpolated concentrations of native Alpha-synuclein in human serum and plasmas samples.

The concentrations of Alpha-synuclein were measured in duplicates, interpolated from the Alpha-synuclein standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 100%, plasma (EDTA) 100%, plasma (citrate) 50% and plasma (heparin) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Alpha-synuclein concentration was determined to be 6.6 ng/mL in serum, 6.8 ng/mL in plasma (EDTA), 13.6 ng/mL in plasma (citrate), and 7.1 ng/mL in plasma (heparin).

Sandwich ELISA - Human Alpha-synuclein ELISA Kit (AB260052)
  • sELISA

Supplier Data

Sandwich ELISA - Human Alpha-synuclein ELISA Kit (AB260052)

Interpolated concentrations of native Alpha-synuclein in human brain and SH-SY5Y cell extract based on a 3 μg/mL and 500 µg/mL extract load, respectfully.

The concentrations of Alpha-synuclein were measured in duplicate and interpolated from the Alpha-synuclein standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Sandwich ELISA - Human Alpha-synuclein ELISA Kit (AB260052)
  • sELISA

Supplier Data

Sandwich ELISA - Human Alpha-synuclein ELISA Kit (AB260052)

Assay sensitivity.

The MDD was determined by calculating the mean of zero standard replicates and adding 2 standard deviations then extrapolating the corresponding concentration.

Sandwich ELISA - Human Alpha-synuclein ELISA Kit (AB260052)
  • sELISA

Supplier Data

Sandwich ELISA - Human Alpha-synuclein ELISA Kit (AB260052)

Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.

Native Alpha-synuclein was measured in the following biological samples in a 1.5-fold dilution series. Sample dilutions are made in Sample Diluent 1X Cell Extraction Buffer PTR.

Recombinant Alpha-synuclein protein was spiked into RPMI media containing 10% FBS in a 1.5 -fold dilution series. Serial dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human Alpha-synuclein ELISA Kit (AB260052)
  • sELISA

Supplier Data

Sandwich ELISA - Human Alpha-synuclein ELISA Kit (AB260052)

Example of human Alpha-synuclein standard curve in Sample Diluent NS.

The Alpha-synuclein standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Alpha-synuclein ELISA Kit (AB260052)
  • sELISA

Supplier Data

Sandwich ELISA - Human Alpha-synuclein ELISA Kit (AB260052)

Example of human Alpha-synuclein standard curve in 1X Cell Extraction Buffer PTR.

The Alpha-synuclein standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed

Sandwich ELISA - Human Alpha-synuclein ELISA Kit (AB260052)
  • sELISA

Supplier Data

Sandwich ELISA - Human Alpha-synuclein ELISA Kit (AB260052)

Example of human Alpha-synuclein standard curve in Sample Diluent NS.

The Alpha-synuclein standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Alpha-synuclein ELISA Kit (AB260052)
  • sELISA

Supplier Data

Sandwich ELISA - Human Alpha-synuclein ELISA Kit (AB260052)

Two-fold serial dilutions of monomer and filament Alpha-synuclein forms were measured in duplicate in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Tissue Lysate, Cell culture media, Heparin Plasma, Citrate plasma, Serum, Cell Lysate, EDTA Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 504 pg/mL

Range

615 - 7000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Alpha-synuclein ELISA Kit ab260052 is a rapid single-wash 90-min ELISA kit to measure Human Alpha-synuclein in Tissue lysate, cell lysate, cell culture media, cell culture supernatant, plasma and serum. This SimpleStep ELISA® sensitivity is 504 pg/mL.

How the assay works

Human Alpha-synuclein SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

This kit recognizes both native and recombinant human human α-Synuclein protein in serum, plasma, and cell culture supernatant, cell and tissue extract samples only. Urine, saliva, and cerebrospinal fluid samples have not been tested with this kit. Both monomeric and aggregated Alpha-synuclein can be detected with this kit.

Human Alpha-synuclein ELISA Kit ab260052 protocol summary:

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm

How other researchers are using Human Alpha-synuclein ELISA kit ab260052

Human Alpha-synuclein ELISA kit ab260052 has been used in publications to study a targeted protein degradation platform for developing drugs to delay symptoms of Parkinson's Disease(1), the correlation of alpha-synuclein levels with neuronal thread protein and apolipoprotein E in Alzheimer's Disease(2), and metabolic reprogramming of misfolded proteins in neurodegenerative disorders using polyphenol(3).

References:

(1)Young Ho Suh et al. 2023 PMID: 37355598,
(2)Dantao Peng et al. 2021 PMID: 34312362,
(3)Hongzhuan Chen et al. 2023 PMID: 36873190

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation

- Human Alpha-synuclein Antibody Pair - BSA and Azide free ab270346
- Anti-Alpha-synuclein antibody [MJFR-14-6-4-2] - BSA and Azide free (Capture) ab259611
- Anti-Alpha-synuclein antibody [MJFR1] - BSA and Azide free (Capture/Detector) ab259612

Related and recommended products

Human Alpha-synuclein ELISA kit ab260052 is commonly used to study neurodegenerative diseases such as Parkinson's disease and Lewy body dementia.

Other kits for measuring neurodegeneration include:

- Human IL-1 beta ELISA kit ab214025
- Human Amyloid beta 1-40 ELISA kit ab193692
- Human Amyloid Beta 42 ELISA kit ab289832

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.9" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.5" } ]
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Recovery

[ { "sample": "Serum", "range": "116 - 118 %", "average": "= 117" }, { "sample": "EDTA Plasma", "range": "105 - 129 %", "average": "= 118" }, { "sample": "Cell culture media", "range": "90 - 114 %", "average": "= 103" }, { "sample": "Heparin Plasma", "range": "93 - 115 %", "average": "= 106" }, { "sample": "Citrate plasma", "range": "113 - 113 %", "average": "= 113" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha-synuclein often referred to by alternate names such as SNCA is a protein of around 14 kDa mass. It mainly expresses in the brain particularly in presynaptic nerve terminals. This protein functions mechanically by stabilizing synaptic vesicles and maintaining synaptic function. It exists both in soluble monomer forms and as aggregates in protein filaments. Antibodies like 4D6 and EP1536Y target monomer forms of protein for more detailed studies.
Biological function summary

The alpha-synuclein protein plays critical roles in neuronal activity. It contributes to neurotransmitter release regulation by acting in the formation and plasticity of the presynaptic neuronal network. Alpha-synuclein doesn't usually form parts of large protein complexes but it may associate transiently with membranes and vesicular structures. The protein's monomer form has also been observed in alpha lines and related neuronal processes operating alongside various cellular functions.

Pathways

Synaptic vesicle trafficking and dopamine neurotransmitter release are significant areas involving the alpha-synuclein protein. In these pathways alpha-synuclein interacts with other proteins like synaptophysin and protein monomer monomerizations are intrinsic to these processes. Altered function or aggregation of alpha-synuclein disrupts these pathways influencing broader neurological functions.

Alterations or accumulations of alpha-synuclein are strongly linked to Parkinson's disease and Lewy body dementia. In these conditions alpha-synuclein forms abnormal protein filaments known as Lewy bodies within neurons. These formations disrupt cellular processes and neuron health. Synucleinopathies such as these show connections with proteins like parkin and DJ-1 which also have key roles in these neurodegenerative diseases.
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Product protocols

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Target data

The protein expressed by the SNCA gene is involved in various synaptic activities, including the regulation of synaptic vesicle trafficking and neurotransmitter release. As a monomer, it enhances synaptic vesicle exocytosis through vesicle priming, fusion, and dilation of exocytotic fusion pores. Mechanistically, it increases local Ca(2+) release, which is crucial for ATP-induced exocytosis. In its multimeric membrane-bound form, SNCA acts as a molecular chaperone, assisting in the folding of synaptic fusion components known as SNAREs at the presynaptic plasma membrane, in conjunction with cysteine string protein-alpha/DNAJC5, a function that is vital for maintaining normal SNARE-complex assembly during aging. Additionally, SNCA plays a role in dopamine neurotransmission regulation by associating with the dopamine transporter (DAT1) and modulating its activity. This supplementary information is collated from multiple sources and compiled automatically.
See full target information SNCA
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Molecular neurodegeneration 18:41 PubMed37355598

2023

Targeted degradation of ⍺-synuclein aggregates in Parkinson's disease using the AUTOTAC technology.

Applications

Unspecified application

Species

Unspecified reactive species

Jihoon Lee,Ki Woon Sung,Eun-Jin Bae,Dabin Yoon,Dasarang Kim,Jin Saem Lee,Da-Ha Park,Daniel Youngjae Park,Su Ran Mun,Soon Chul Kwon,Hye Yeon Kim,Joo-Ok Min,Seung-Jae Lee,Young Ho Suh,Yong Tae Kwon

The Journal of clinical investigation 133: PubMed37014703

2023

Accumulation of α-synuclein mediates podocyte injury in Fabry nephropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Fabian Braun,Ahmed Abed,Dominik Sellung,Manuel Rogg,Mathias Woidy,Oysten Eikrem,Nicola Wanner,Jessica Gambardella,Sandra D Laufer,Fabian Haas,Milagros N Wong,Bernhard Dumoulin,Paula Rischke,Anne Mühlig,Wiebke Sachs,Katharina von Cossel,Kristina Schulz,Nicole Muschol,Sören W Gersting,Ania C Muntau,Oliver Kretz,Oliver Hahn,Markus M Rinschen,Michael Mauer,Tillmann Bork,Florian Grahammer,Wei Liang,Thorsten Eierhoff,Winfried Römer,Arne Hansen,Catherine Meyer-Schwesinger,Guido Iaccarino,Camilla Tøndel,Hans-Peter Marti,Behzad Najafian,Victor G Puelles,Christoph Schell,Tobias B Huber

Acta pharmaceutica Sinica. B 13:834-851 PubMed36873190

2023

Nanopolyphenol rejuvenates microglial surveillance of multiple misfolded proteins through metabolic reprogramming.

Applications

Unspecified application

Species

Unspecified reactive species

Dayuan Wang,Xiao Gu,Xinyi Ma,Jun Chen,Qizhi Zhang,Zhihua Yu,Juan Li,Meng Hu,Xiaofang Tan,Yuyun Tang,Jianrong Xu,Minjun Xu,Qingxiang Song,Huahua Song,Gan Jiang,Zaiming Tang,Xiaoling Gao,Hongzhuan Chen

Frontiers in neurology 13:869103 PubMed35911883

2022

The Quest for Anti-α-Synuclein Antibody Specificity-Lessons Learnt From Flow Cytometry Analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Lukas Leupold,Veronika Sigutova,Elizaveta Gerasimova,Martin Regensburger,Sebastian Zundler,Friederike Zunke,Wei Xiang,Beate Winner,Iryna Prots

Medical science monitor : international medical journal of experimental and clinical research 27:e932998 PubMed34312362

2021

The Association Between Plasma α-Synuclein (α-syn) Protein, Urinary Alzheimer-Associated Neuronal Thread Protein (AD7c-NTP), and Apolipoprotein Epsilon 4 (ApoE ε4) Alleles and Cognitive Decline in 60 Patients with Alzheimer's Disease Compared with 28 Age-Matched Normal Individuals.

Applications

Unspecified application

Species

Unspecified reactive species

Shuang Lv,Xiao Zhou,Yiming Li,Shujuan Zhang,Yu Wang,Shuhong Jia,Xiaoqian Niu,Lei Wang,Dantao Peng

Communications biology 4:613 PubMed34021258

2021

Super-resolution imaging reveals α-synuclein seeded aggregation in SH-SY5Y cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jason C Sang,Eric Hidari,Georg Meisl,Rohan T Ranasinghe,Maria Grazia Spillantini,David Klenerman
View all publications
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We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Alternative Version
Assay Kits

AB270346

Human Alpha-synuclein Antibody Pair - BSA and Azide free

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human-alpha-synuclein-antibody-pair-bsa-and-azide-free-ab270346

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