The Human Alpha-synuclein ELISA kit is a single-wash, 90-minute SimpleStep ELISA® assay designed for the quantification of human alpha-synuclein (SNCa) with a sensitivity of 504 pg/mL. The assay employs a simple Mix-Wash-Read protocol with just one incubation and wash step.Easy-to-use, rapid, sensitive human SNCa sandwich ELISA kit:- Colorimetric assay with a 450 nm readout; compatible with any standard plate reader- Available in different formats to meet various throughput needs, including 384-well plates for higher throughput- Broad sample compatibility – suitable for serum, plasma (heparin, citrate, EDTA), cell culture supernatants, cell lysates, and tissue extracts- KO validated for guaranteed specificity
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- Molecular neurodegeneration 18:412023Targeted degradation of ⍺-synuclein aggregates in Parkinson's disease using the AUTOTAC technology.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJihoon Lee,Ki Woon Sung,Eun-Jin Bae,Dabin Yoon,Dasarang Kim,Jin Saem Lee,Da-Ha Park,Daniel Youngjae Park,Su Ran Mun,Soon Chul Kwon,Hye Yeon Kim,Joo-Ok Min,Seung-Jae Lee,Young Ho Suh,Yong Tae KwonPubMed 37355598
- The Journal of clinical investigation 133:2023Accumulation of α-synuclein mediates podocyte injury in Fabry nephropathy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFabian Braun,Ahmed Abed,Dominik Sellung,Manuel Rogg,Mathias Woidy,Oysten Eikrem,Nicola Wanner,Jessica Gambardella,Sandra D Laufer,Fabian Haas,Milagros N Wong,Bernhard Dumoulin,Paula Rischke,Anne Mühlig,Wiebke Sachs,Katharina von Cossel,Kristina Schulz,Nicole Muschol,Sören W Gersting,Ania C Muntau,Oliver Kretz,Oliver Hahn,Markus M Rinschen,Michael Mauer,Tillmann Bork,Florian Grahammer,Wei Liang,Thorsten Eierhoff,Winfried Römer,Arne Hansen,Catherine Meyer-Schwesinger,Guido Iaccarino,Camilla Tøndel,Hans-Peter Marti,Behzad Najafian,Victor G Puelles,Christoph Schell,Tobias B HuberPubMed 37014703
- Acta pharmaceutica Sinica. B 13:834-8512023Nanopolyphenol rejuvenates microglial surveillance of multiple misfolded proteins through metabolic reprogramming.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDayuan Wang,Xiao Gu,Xinyi Ma,Jun Chen,Qizhi Zhang,Zhihua Yu,Juan Li,Meng Hu,Xiaofang Tan,Yuyun Tang,Jianrong Xu,Minjun Xu,Qingxiang Song,Huahua Song,Gan Jiang,Zaiming Tang,Xiaoling Gao,Hongzhuan ChenPubMed 36873190
- Frontiers in neurology 13:8691032022The Quest for Anti-α-Synuclein Antibody Specificity-Lessons Learnt From Flow Cytometry Analysis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLukas Leupold,Veronika Sigutova,Elizaveta Gerasimova,Martin Regensburger,Sebastian Zundler,Friederike Zunke,Wei Xiang,Beate Winner,Iryna ProtsPubMed 35911883
- Medical science monitor : international medical journal of experimental and clinical research 27:e9329982021The Association Between Plasma α-Synuclein (α-syn) Protein, Urinary Alzheimer-Associated Neuronal Thread Protein (AD7c-NTP), and Apolipoprotein Epsilon 4 (ApoE ε4) Alleles and Cognitive Decline in 60 Patients with Alzheimer's Disease Compared with 28 Age-Matched Normal Individuals.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShuang Lv,Xiao Zhou,Yiming Li,Shujuan Zhang,Yu Wang,Shuhong Jia,Xiaoqian Niu,Lei Wang,Dantao PengPubMed 34312362
- Communications biology 4:6132021Super-resolution imaging reveals α-synuclein seeded aggregation in SH-SY5Y cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJason C Sang,Eric Hidari,Georg Meisl,Rohan T Ranasinghe,Maria Grazia Spillantini,David KlenermanPubMed 34021258
Key facts
- Detection method
Colorimetric
- Sample types
Tissue Lysate, Cell culture media, Heparin Plasma, Citrate plasma, Serum, Cell Lysate, EDTA Plasma, Cell culture supernatant
- Assay type
Sandwich (quantitative)
- Reactive species
Human
- Range
615 - 7000 pg/mL
- Assay time
1h 30m
- Sensitivity
= 504 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Neuronal protein that plays several roles in synaptic activity such as regulation of synaptic vesicle trafficking and subsequent neurotransmitter release (PubMed:20798282, PubMed:26442590, PubMed:28288128, PubMed:30404828). Participates as a monomer in synaptic vesicle exocytosis by enhancing vesicle priming, fusion and dilation of exocytotic fusion pores (PubMed:28288128, PubMed:30404828). Mechanistically, acts by increasing local Ca(2+) release from microdomains which is essential for the enhancement of ATP-induced exocytosis (PubMed:30404828). Acts also as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of synaptic fusion components called SNAREs (Soluble NSF Attachment Protein REceptors) at presynaptic plasma membrane in conjunction with cysteine string protein-alpha/DNAJC5 (PubMed:20798282). This chaperone activity is important to sustain normal SNARE-complex assembly during aging (PubMed:20798282). Also plays a role in the regulation of the dopamine neurotransmission by associating with the dopamine transporter (DAT1) and thereby modulating its activity (PubMed:26442590).
Alternative names
NACP, PARK1, SNCA, Alpha-synuclein, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor, NACP
What's included?
Recommended products
The Human Alpha-synuclein ELISA kit is a single-wash, 90-minute SimpleStep ELISA® assay designed for the quantification of human alpha-synuclein (SNCa) with a sensitivity of 504 pg/mL. The assay employs a simple Mix-Wash-Read protocol with just one incubation and wash step.Easy-to-use, rapid, sensitive human SNCa sandwich ELISA kit:- Colorimetric assay with a 450 nm readout; compatible with any standard plate reader- Available in different formats to meet various throughput needs, including 384-well plates for higher throughput- Broad sample compatibility – suitable for serum, plasma (heparin, citrate, EDTA), cell culture supernatants, cell lysates, and tissue extracts- KO validated for guaranteed specificity
Key facts
- Detection method
Colorimetric
- Sample types
Tissue Lysate, Cell culture media, Heparin Plasma, Citrate plasma, Serum, Cell Lysate, EDTA Plasma, Cell culture supernatant
- Assay type
Sandwich (quantitative)
- Reactive species
Human
- Range
615 - 7000 pg/mL
- Assay time
1h 30m
- Assay Platform
Pre-coated microplate (12 x 8 well strips)
- Sensitivity
= 504 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 8 | mean - | SD - | C.V. 2.5 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. 8.9 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 117 | Range 116 - 118 % |
Sample type EDTA Plasma | Average % = 118 | Range 105 - 129 % |
Sample type Cell culture media | Average % = 103 | Range 90 - 114 % |
Sample type Heparin Plasma | Average % = 106 | Range 93 - 115 % |
Sample type Citrate plasma | Average % = 113 | Range 113 - 113 % |
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
- Storage information
+4°C
Notes
Human Alpha-synuclein ELISA Kit (ab260052) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Alpha-synuclein protein in cell culture media, cell lysate, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Alpha-synuclein with 504 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
ASSAY SPECIFICITY
This kit recognizes both native and recombinant human Alpha-synuclein protein in serum, plasma, and cell culture supernatant, cell and tissue extract samples only.
Urine, saliva, and cerebrospinal fluid samples have not been tested with this kit.
Both monomeric and aggregated Alpha-synuclein can be detected with this kit.
CROSS REACTIVITY
Recombinant Tau 441, UCHL-1, beta-tubulin, and Gamma-synuclein were prepared at 7,000 pg/mL and assayed for cross reactivity. No cross-reactivity was observed.
INTERFERENCE
Recombinant Tau 441, UCHL-1, and beta tubulin were prepared at 4 ng/mL and tested for interference. No interference was observed.
SPECIES REACTIVITY
This kit recognizes human Alpha-synuclein protein.
Other species reactivity was determined by measuring 50% serum samples of various species, interpolating the protein concentrations from the human standard curve, and expressing the interpolated concentrations as a percentage of the protein concentration in human serum assayed at the same dilution.
This kit was found to react with rhesus macaque serum.
Reactivity < 3% was determined for the following species:
- Mouse
- Rat
- Cow
Other species reactivity not determined.
Browse our curated portfolio of extensively validated recombinant antibodies and assays sensitive to Tau, Beta-amyloid, and other Alzheimer’s targets of interest, with everything you need in one place.
Alpha-synuclein is a 140- amino acid protein encoded by the SNCA gene, primarily found in neural tissue. It is a member of the synuclein family of proteins, including β-Synuclein and γ-Synuclein. Alpha-synuclein has been found concentrated in the presynaptic nerve terminals of neurons and has been found in the nucleus of neurons. Alpha-synuclein is a major component of Lewy Bodies. This protein appears to play a role in dopamine metabolism and vesicle trafficking.
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It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Alpha-synuclein often referred to by alternate names such as SNCA is a protein of around 14 kDa mass. It mainly expresses in the brain particularly in presynaptic nerve terminals. This protein functions mechanically by stabilizing synaptic vesicles and maintaining synaptic function. It exists both in soluble monomer forms and as aggregates in protein filaments. Antibodies like 4D6 and EP1536Y target monomer forms of protein for more detailed studies.
- Biological function summary
The alpha-synuclein protein plays critical roles in neuronal activity. It contributes to neurotransmitter release regulation by acting in the formation and plasticity of the presynaptic neuronal network. Alpha-synuclein doesn't usually form parts of large protein complexes but it may associate transiently with membranes and vesicular structures. The protein's monomer form has also been observed in alpha lines and related neuronal processes operating alongside various cellular functions.
- Pathways
Synaptic vesicle trafficking and dopamine neurotransmitter release are significant areas involving the alpha-synuclein protein. In these pathways alpha-synuclein interacts with other proteins like synaptophysin and protein monomer monomerizations are intrinsic to these processes. Altered function or aggregation of alpha-synuclein disrupts these pathways influencing broader neurological functions.
- Associated diseases and disorders
Alterations or accumulations of alpha-synuclein are strongly linked to Parkinson's disease and Lewy body dementia. In these conditions alpha-synuclein forms abnormal protein filaments known as Lewy bodies within neurons. These formations disrupt cellular processes and neuron health. Synucleinopathies such as these show connections with proteins like parkin and DJ-1 which also have key roles in these neurodegenerative diseases.
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13 product images
Sandwich ELISA - Human Alpha-synuclein ELISA Kit (ab260052)
Human Alpha Synuclein rabmab remake comparison.
Standard Curve comparison between the original Human Alpha-synuclein SimpleStep ELISA kit (ab210973) and current Human Alpha-synuclein SimpleStep ELISA kit (ab260052).
Sandwich ELISA - Human Alpha-synuclein ELISA Kit (ab260052)
Example of human Alpha-synuclein standard curve in Sample Diluent NS.
The Alpha-synuclein standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Alpha-synuclein ELISA Kit (ab260052)
Example of human Alpha-synuclein standard curve in Sample Diluent NS.
The Alpha-synuclein standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Alpha-synuclein ELISA Kit (ab260052)
Example of human Alpha-synuclein standard curve in 1X Cell Extraction Buffer PTR.
The Alpha-synuclein standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed
Sandwich ELISA - Human Alpha-synuclein ELISA Kit (ab260052)
Example of human Alpha-synuclein standard curve in 1X Cell Extraction Buffer PTR.
The Alpha-synuclein standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Alpha-synuclein ELISA Kit (ab260052)
Interpolated concentrations of native Alpha-synuclein in human serum and plasmas samples.
The concentrations of Alpha-synuclein were measured in duplicates, interpolated from the Alpha-synuclein standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 100%, plasma (EDTA) 100%, plasma (citrate) 50% and plasma (heparin) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Alpha-synuclein concentration was determined to be 6.6 ng/mL in serum, 6.8 ng/mL in plasma (EDTA), 13.6 ng/mL in plasma (citrate), and 7.1 ng/mL in plasma (heparin).
Sandwich ELISA - Human Alpha-synuclein ELISA Kit (ab260052)
Interpolated concentrations of native Alpha-synuclein in human brain and SH-SY5Y cell extract based on a 3 μg/mL and 500 µg/mL extract load, respectfully.
The concentrations of Alpha-synuclein were measured in duplicate and interpolated from the Alpha-synuclein standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Sandwich ELISA - Human Alpha-synuclein ELISA Kit (ab260052)
Serum from eight individual healthy human female donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Alpha-synuclein concentration was determined to be 7.8 ng/mL with a range of 3.0 – 8.9 ng/mL.
Sandwich ELISA - Human Alpha-synuclein ELISA Kit (ab260052)
Raw data is plotted for wild-type and alpha synuclein-knockout HAP1 cell extracts tested at 111 μg/mL.
Sandwich ELISA - Human Alpha-synuclein ELISA Kit (ab260052)
Assay sensitivity.
The MDD was determined by calculating the mean of zero standard replicates and adding 2 standard deviations then extrapolating the corresponding concentration.
Sandwich ELISA - Human Alpha-synuclein ELISA Kit (ab260052)
Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.
Native Alpha-synuclein was measured in the following biological samples in a 1.5-fold dilution series. Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Human Alpha-synuclein ELISA Kit (ab260052)
Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.
Native Alpha-synuclein was measured in the following biological samples in a 1.5-fold dilution series. Sample dilutions are made in Sample Diluent 1X Cell Extraction Buffer PTR.
Recombinant Alpha-synuclein protein was spiked into RPMI media containing 10% FBS in a 1.5 -fold dilution series. Serial dilutions are made in Sample Diluent NS.
Sandwich ELISA - Human Alpha-synuclein ELISA Kit (ab260052)
Two-fold serial dilutions of monomer and filament Alpha-synuclein forms were measured in duplicate in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.
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