Human Alpha-synuclein ELISA kit is a single-wash, 90-minute SimpleStep ELISA® used to quantify human alpha-synuclein (SNCa) with a sensitivity of 504 pg/mL. The assay employs a simple Mix-Wash-Read protocol with just one incubation and wash step.
- Colorimetric sandwich ELISA with a 450 nm readout; compatible with any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready carrier-free antibody pair
- KO validated for guaranteed specificity
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- Molecular neurodegeneration 18:412023Targeted degradation of ⍺-synuclein aggregates in Parkinson's disease using the AUTOTAC technology.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJihoon Lee,Ki Woon Sung,Eun-Jin Bae,Dabin Yoon,Dasarang Kim,Jin Saem Lee,Da-Ha Park,Daniel Youngjae Park,Su Ran Mun,Soon Chul Kwon,Hye Yeon Kim,Joo-Ok Min,Seung-Jae Lee,Young Ho Suh,Yong Tae KwonPubMed 37355598
- The Journal of clinical investigation 133:2023Accumulation of α-synuclein mediates podocyte injury in Fabry nephropathy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFabian Braun,Ahmed Abed,Dominik Sellung,Manuel Rogg,Mathias Woidy,Oysten Eikrem,Nicola Wanner,Jessica Gambardella,Sandra D Laufer,Fabian Haas,Milagros N Wong,Bernhard Dumoulin,Paula Rischke,Anne Mühlig,Wiebke Sachs,Katharina von Cossel,Kristina Schulz,Nicole Muschol,Sören W Gersting,Ania C Muntau,Oliver Kretz,Oliver Hahn,Markus M Rinschen,Michael Mauer,Tillmann Bork,Florian Grahammer,Wei Liang,Thorsten Eierhoff,Winfried Römer,Arne Hansen,Catherine Meyer-Schwesinger,Guido Iaccarino,Camilla Tøndel,Hans-Peter Marti,Behzad Najafian,Victor G Puelles,Christoph Schell,Tobias B HuberPubMed 37014703
- Acta pharmaceutica Sinica. B 13:834-8512023Nanopolyphenol rejuvenates microglial surveillance of multiple misfolded proteins through metabolic reprogramming.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDayuan Wang,Xiao Gu,Xinyi Ma,Jun Chen,Qizhi Zhang,Zhihua Yu,Juan Li,Meng Hu,Xiaofang Tan,Yuyun Tang,Jianrong Xu,Minjun Xu,Qingxiang Song,Huahua Song,Gan Jiang,Zaiming Tang,Xiaoling Gao,Hongzhuan ChenPubMed 36873190
- Frontiers in neurology 13:8691032022The Quest for Anti-α-Synuclein Antibody Specificity-Lessons Learnt From Flow Cytometry Analysis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLukas Leupold,Veronika Sigutova,Elizaveta Gerasimova,Martin Regensburger,Sebastian Zundler,Friederike Zunke,Wei Xiang,Beate Winner,Iryna ProtsPubMed 35911883
- Medical science monitor : international medical journal of experimental and clinical research 27:e9329982021The Association Between Plasma α-Synuclein (α-syn) Protein, Urinary Alzheimer-Associated Neuronal Thread Protein (AD7c-NTP), and Apolipoprotein Epsilon 4 (ApoE ε4) Alleles and Cognitive Decline in 60 Patients with Alzheimer's Disease Compared with 28 Age-Matched Normal Individuals.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShuang Lv,Xiao Zhou,Yiming Li,Shujuan Zhang,Yu Wang,Shuhong Jia,Xiaoqian Niu,Lei Wang,Dantao PengPubMed 34312362
- Communications biology 4:6132021Super-resolution imaging reveals α-synuclein seeded aggregation in SH-SY5Y cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJason C Sang,Eric Hidari,Georg Meisl,Rohan T Ranasinghe,Maria Grazia Spillantini,David KlenermanPubMed 34021258
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue Lysate, Cell culture media, Heparin Plasma, Citrate plasma, Serum, Cell Lysate, EDTA Plasma, Cell culture supernatant
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 615 - 7000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 504 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
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Target data
Function
The protein expressed by the SNCA gene is involved in various synaptic activities, including the regulation of synaptic vesicle trafficking and neurotransmitter release. As a monomer, it enhances synaptic vesicle exocytosis through vesicle priming, fusion, and dilation of exocytotic fusion pores. Mechanistically, it increases local Ca(2+) release, which is crucial for ATP-induced exocytosis. In its multimeric membrane-bound form, SNCA acts as a molecular chaperone, assisting in the folding of synaptic fusion components known as SNAREs at the presynaptic plasma membrane, in conjunction with cysteine string protein-alpha/DNAJC5, a function that is vital for maintaining normal SNARE-complex assembly during aging. Additionally, SNCA plays a role in dopamine neurotransmission regulation by associating with the dopamine transporter (DAT1) and modulating its activity. This supplementary information is collated from multiple sources and compiled automatically.
Alternative names
NACP, PARK1, SNCA, Alpha-synuclein, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor
What's included?
Recommended products
Human Alpha-synuclein ELISA kit is a single-wash, 90-minute SimpleStep ELISA® used to quantify human alpha-synuclein (SNCa) with a sensitivity of 504 pg/mL. The assay employs a simple Mix-Wash-Read protocol with just one incubation and wash step.
- Colorimetric sandwich ELISA with a 450 nm readout; compatible with any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready carrier-free antibody pair
- KO validated for guaranteed specificity
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue Lysate, Cell culture media, Heparin Plasma, Citrate plasma, Serum, Cell Lysate, EDTA Plasma, Cell culture supernatant
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 615 - 7000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 504 pg/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 8 | C.V. 2.5 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 3 | C.V. 8.9 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 117 | Range 116 - 118 % |
Sample type EDTA Plasma | Average % = 118 | Range 105 - 129 % |
Sample type Cell culture media | Average % = 103 | Range 90 - 114 % |
Sample type Heparin Plasma | Average % = 106 | Range 93 - 115 % |
Sample type Citrate plasma | Average % = 113 | Range 113 - 113 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human Alpha-synuclein ELISA Kit ab260052 is a rapid single-wash 90-min ELISA kit to measure Human Alpha-synuclein in Tissue lysate, cell lysate, cell culture media, cell culture supernatant, plasma and serum. This SimpleStep ELISA® sensitivity is 504 pg/mL.
How the assay works
Human Alpha-synuclein SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
This kit recognizes both native and recombinant human human α-Synuclein protein in serum, plasma, and cell culture supernatant, cell and tissue extract samples only. Urine, saliva, and cerebrospinal fluid samples have not been tested with this kit. Both monomeric and aggregated Alpha-synuclein can be detected with this kit.
Human Alpha-synuclein ELISA Kit ab260052 protocol summary:
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm
How other researchers are using Human Alpha-synuclein ELISA kit ab260052
Human Alpha-synuclein ELISA kit ab260052 has been used in publications to study a targeted protein degradation platform for developing drugs to delay symptoms of Parkinson's Disease(1), the correlation of alpha-synuclein levels with neuronal thread protein and apolipoprotein E in Alzheimer's Disease(2), and metabolic reprogramming of misfolded proteins in neurodegenerative disorders using polyphenol(3).
References:
(1)Young Ho Suh et al. 2023 PMID: 37355598,
(2)Dantao Peng et al. 2021 PMID: 34312362,
(3)Hongzhuan Chen et al. 2023 PMID: 36873190
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation
- Human Alpha-synuclein Antibody Pair - BSA and Azide free Human Alpha-synuclein Antibody Pair - BSA and Azide free ab270346
- Anti-Alpha-synuclein antibody [MJFR-14-6-4-2] - BSA and Azide free (Capture) Anti-Alpha-synuclein antibody [MJFR-14-6-4-2] - BSA and Azide free (Capture) ab259611
- Anti-Alpha-synuclein antibody [MJFR1] - BSA and Azide free (Capture/Detector) Anti-Alpha-synuclein antibody [MJFR1] - BSA and Azide free (Capture/Detector) ab259612
Related and recommended products
Human Alpha-synuclein ELISA kit ab260052 is commonly used to study neurodegenerative diseases such as Parkinson's disease and Lewy body dementia.
Other kits for measuring neurodegeneration include:
- Human IL-1 beta ELISA kit Human IL-1 beta ELISA Kit ab214025
- Human Amyloid beta 1-40 ELISA kit Human Amyloid beta 1-40 ELISA Kit ab193692
- Human Amyloid Beta 42 ELISA kit Human Amyloid Beta 42 ELISA Kit ab289832
REACH authorisation
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It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Product promise
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14 product images
ELISA - Human Alpha-synuclein ELISA Kit (ab260052)
Sandwich ELISA - Human Alpha-synuclein ELISA Kit (ab260052)
Human Alpha Synuclein rabmab remake comparison.
Standard Curve comparison between the original Human Alpha-synuclein SimpleStep ELISA kit (ab210973) and current Human Alpha-synuclein SimpleStep ELISA kit (ab260052).
Sandwich ELISA - Human Alpha-synuclein ELISA Kit (ab260052)
Example of human Alpha-synuclein standard curve in Sample Diluent NS.
The Alpha-synuclein standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Alpha-synuclein ELISA Kit (ab260052)
Example of human Alpha-synuclein standard curve in Sample Diluent NS.
The Alpha-synuclein standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Alpha-synuclein ELISA Kit (ab260052)
Example of human Alpha-synuclein standard curve in 1X Cell Extraction Buffer PTR.
The Alpha-synuclein standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed
Sandwich ELISA - Human Alpha-synuclein ELISA Kit (ab260052)
Example of human Alpha-synuclein standard curve in 1X Cell Extraction Buffer PTR.
The Alpha-synuclein standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Alpha-synuclein ELISA Kit (ab260052)
Interpolated concentrations of native Alpha-synuclein in human serum and plasmas samples.
The concentrations of Alpha-synuclein were measured in duplicates, interpolated from the Alpha-synuclein standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 100%, plasma (EDTA) 100%, plasma (citrate) 50% and plasma (heparin) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Alpha-synuclein concentration was determined to be 6.6 ng/mL in serum, 6.8 ng/mL in plasma (EDTA), 13.6 ng/mL in plasma (citrate), and 7.1 ng/mL in plasma (heparin).
Sandwich ELISA - Human Alpha-synuclein ELISA Kit (ab260052)
Interpolated concentrations of native Alpha-synuclein in human brain and SH-SY5Y cell extract based on a 3 μg/mL and 500 µg/mL extract load, respectfully.
The concentrations of Alpha-synuclein were measured in duplicate and interpolated from the Alpha-synuclein standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Sandwich ELISA - Human Alpha-synuclein ELISA Kit (ab260052)
Serum from eight individual healthy human female donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Alpha-synuclein concentration was determined to be 7.8 ng/mL with a range of 3.0 – 8.9 ng/mL.
Sandwich ELISA - Human Alpha-synuclein ELISA Kit (ab260052)
Raw data is plotted for wild-type and alpha synuclein-knockout HAP1 cell extracts tested at 111 μg/mL.
Sandwich ELISA - Human Alpha-synuclein ELISA Kit (ab260052)
Assay sensitivity.
The MDD was determined by calculating the mean of zero standard replicates and adding 2 standard deviations then extrapolating the corresponding concentration.
Sandwich ELISA - Human Alpha-synuclein ELISA Kit (ab260052)
Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.
Native Alpha-synuclein was measured in the following biological samples in a 1.5-fold dilution series. Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Human Alpha-synuclein ELISA Kit (ab260052)
Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.
Native Alpha-synuclein was measured in the following biological samples in a 1.5-fold dilution series. Sample dilutions are made in Sample Diluent 1X Cell Extraction Buffer PTR.
Recombinant Alpha-synuclein protein was spiked into RPMI media containing 10% FBS in a 1.5 -fold dilution series. Serial dilutions are made in Sample Diluent NS.
Sandwich ELISA - Human Alpha-synuclein ELISA Kit (ab260052)
Two-fold serial dilutions of monomer and filament Alpha-synuclein forms were measured in duplicate in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.
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