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AB234578

Human ALT ELISA Kit

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(1 Review)

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(5 Publications)

Human ALT ELISA kit is a single-wash, 90-minute SimpleStep ELISA® used to quantify human alanine aminotransferase (ALT) with a sensitivity of 47.34 pg/mL. The assay employs a simple Mix-Wash-Read protocol with just one incubation and wash step.

- Colorimetric sandwich ELISA with a 450 nm readout; compatible with any standard plate reader
- Different formats for different needs, including 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready carrier-free antibody pair

View Alternative Names

AAT1, GPT1, GPT, Alanine aminotransferase 1, ALT1, Glutamate pyruvate transaminase 1, Glutamic--alanine transaminase 1, Glutamic--pyruvic transaminase 1, GPT 1

8 Images
Sandwich ELISA - Human ALT ELISA Kit (AB234578)
  • sELISA

Lab

Sandwich ELISA - Human ALT ELISA Kit (AB234578)

SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

Sandwich ELISA - Human ALT ELISA Kit (AB234578)
  • sELISA

Lab

Sandwich ELISA - Human ALT ELISA Kit (AB234578)

Example of human ALT standard curve in Sample Diluent NS in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human ALT ELISA Kit (AB234578)
  • sELISA

Lab

Sandwich ELISA - Human ALT ELISA Kit (AB234578)

Interpolated concentration of native ALT was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 25% serum. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS + 1X Enhancer.

Sandwich ELISA - Human ALT ELISA Kit (AB234578)
  • sELISA

Supplier Data

Sandwich ELISA - Human ALT ELISA Kit (AB234578)

Linearity of dilution.

Recombinant ALT was spiked into the following biological samples and diluted in a 2-fold dilution series in Sample Diluent NS + 1X Enhancer.

Sandwich ELISA - Human ALT ELISA Kit (AB234578)
  • sELISA

Supplier Data

Sandwich ELISA - Human ALT ELISA Kit (AB234578)

Interpolated concentrations of native ALT in human serum, plasma (EDTA) and plasma (citrate) samples.

The concentrations of ALT were measured in duplicates, interpolated from the ALT standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 25%, plasma (citrate) 25%, and plasma (EDTA) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ALT concentration was determined to be 4,002.9 pg/mL in serum, 2,685.5 pg/mL in plasma (EDTA) and 1,886.6 pg/mL in plasma (citrate).

Sandwich ELISA - Human ALT ELISA Kit (AB234578)
  • sELISA

Supplier Data

Sandwich ELISA - Human ALT ELISA Kit (AB234578)

Example of human ALT standard curve in Sample Diluent NS + 1X Enhancer. The ALT standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

ELISA - Human ALT ELISA Kit (AB234578)
  • ELISA

Unknown

ELISA - Human ALT ELISA Kit (AB234578)

Example of human ALT standard curve in Sample Diluent NS + Enhancer.

The ALT standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human ALT ELISA Kit (AB234578)
  • sELISA

Supplier Data

Sandwich ELISA - Human ALT ELISA Kit (AB234578)

Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.

Native ALT was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS + 1X Enhancer.

Key facts

Detection method

Colorimetric

Sample types

Cell culture media, Citrate plasma, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 47.34 pg/mL

Range

109.38 - 7000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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You may be interested in:

AB244121

Human ALT Antibody Pair - BSA and Azide free

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0 Reviews

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We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

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Product details

Human ALT ELISA Kit ab234578 is a rapid single-wash 90-min ELISA kit to measure Human ALT in . This SimpleStep ELISA® sensitivity is 47.34 pg/mL.

How the assay works

Human ALT SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

This kit recognizes both native and recombinant human ALT protein in serum, plasma, and cell culture supernatant samples only. Tissue extract samples have not been tested with this kit. This kit is incompatible with plasma- heparin and cell extract samples.

Human ALT ELISA Kit ab234578 protocol summary:

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm

How other researchers are using Human ALT ELISA Kit ab234578

Human ALT ELISA Kit ab234578 has been used to validate a human Liver-Chip as a model for predicting drug-induced liver injury(1)(2) and to study novel therapeutics to hepatitis B(3).

References:

(1)Daniel Levner et al. 2022, PMID: 36473994,
(2)Janey Ronxhi et al. 2022, PMID: 35790095,
(3)Sung-Gyoo Park, et al. 2019, PMID: 31097716

Save your precious samples

Our 384-well Human ALT ELISA Kit only requires a maxmum of 12.5µL

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation

- Human ALT Antibody Pair - BSA and Azide free ab244121
- Anti-Alanine Transaminase antibody [EPR21068-102] - BSA and Azide free (Capture) ab244765
- Anti-Alanine Transaminase antibody [EPR21068-86] - BSA and Azide free (Detector) ab245036

Related and recommended products

Human ALT ELISA kit ab234578 is commonly used by researchers to measure liver function.

Other ELISA kits to measure liver function include:

- Human AST ELISA kit ab263881
- Human Albumin ELISA kit ab179887
- Albumin (BCG) Assay Kit (Colorimetric) ab235628

These ELISA kits are also available for Mouse/Rat:

- Mouse ALT ELISA kit ab282882
- Mouse AST ELISA kit ab263882
- Mouse Albumin ELISA kit ab207620
- Rat ALT ELISA kit abab234579
- Rat AST ELISA kit ab263883
- Rat Albumin ELISA kit ab235642

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.5" } ]
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Recovery

[ { "sample": "Cell culture media", "range": null, "average": "= 95" }, { "sample": "Citrate plasma", "range": null, "average": "= 92" }, { "sample": "EDTA Plasma", "range": null, "average": "= 111" }, { "sample": "Serum", "range": null, "average": "= 117" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alanine transaminase also known as ALT or alanine aminotransferase is an important enzyme in the body. It has a molecular mass of approximately 54 kDa and catalyzes the conversion between alanine and pyruvate by transferring an amino group. ALT is mainly expressed in the liver but also present in other tissues like the heart and muscles. It is often measured as 'ALT activity' in clinical settings to ascertain liver health. In laboratories an 'ALT assay' or 'ALT kit' can determine alanine transaminase activity.
Biological function summary

Alanine transaminase plays a critical role in amino acid metabolism and energy production. It facilitates the transamination process which is significant for the balance of amino acids and carbohydrates. ALT as a standalone enzyme does not form part of a protein complex but works closely with alanine aminotransferase reactions. It helps maintain the cellular nitrogen balance linking nitrogen metabolism with energy pathways.

Pathways

The role of alanine transaminase is to interconnect carbohydrate and amino acid metabolism. It participates in the gluconeogenesis pathway and the citric acid cycle important for energy production. Through its activity in these pathways it interacts with proteins like aspartate aminotransferase (AST) affecting the balance of amino and keto acids in cells.

Alanine transaminase levels indicate liver conditions such as hepatitis and liver cirrhosis. Elevated ALT activity suggests liver damage or inflammation. It also connects to conditions involving muscular injury. In these scenarios increased presence and activity of AST often accompany ALT providing a broader picture of the extent of tissue damage. Understanding the involvement of ALT in these pathways helps diagnose and monitor the progression of relevant health issues.
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Product protocols

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Target data

Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles (By similarity).
See full target information GPT
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Communications medicine 2:154 PubMed36473994

2022

Performance assessment and economic analysis of a human Liver-Chip for predictive toxicology.

Applications

Unspecified application

Species

Unspecified reactive species

Lorna Ewart,Athanasia Apostolou,Skyler A Briggs,Christopher V Carman,Jake T Chaff,Anthony R Heng,Sushma Jadalannagari,Jeshina Janardhanan,Kyung-Jin Jang,Sannidhi R Joshipura,Mahika M Kadam,Marianne Kanellias,Ville J Kujala,Gauri Kulkarni,Christopher Y Le,Carolina Lucchesi,Dimitris V Manatakis,Kairav K Maniar,Meaghan E Quinn,Joseph S Ravan,Ann Catherine Rizos,John F K Sauld,Josiah D Sliz,William Tien-Street,Dennis Ramos Trinidad,James Velez,Max Wendell,Onyi Irrechukwu,Prathap Kumar Mahalingaiah,Donald E Ingber,Jack W Scannell,Daniel Levner

Cell reports 40:111321 PubMed36103835

2022

Human hepatocyte PNPLA3-148M exacerbates rapid non-alcoholic fatty liver disease development in chimeric mice.

Applications

Unspecified application

Species

Unspecified reactive species

Mohammad Kabbani,Eleftherios Michailidis,Sandra Steensels,Clifton G Fulmer,Joseph M Luna,Jérémie Le Pen,Matteo Tardelli,Brandon Razooky,Inna Ricardo-Lax,Chenhui Zou,Briana Zeck,Ansgar F Stenzel,Corrine Quirk,Lander Foquet,Alison W Ashbrook,William M Schneider,Serkan Belkaya,Gadi Lalazar,Yupu Liang,Meredith Pittman,Lindsey Devisscher,Hiroshi Suemizu,Neil D Theise,Luis Chiriboga,David E Cohen,Robert Copenhaver,Markus Grompe,Philip Meuleman,Baran A Ersoy,Charles M Rice,Ype P de Jong

Current protocols 2:e478 PubMed35790095

2022

Co-Culture of Human Primary Hepatocytes and Nonparenchymal Liver Cells in the Emulate® Liver-Chip for the Study of Drug-Induced Liver Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Qiang Shi,Ayesha Arefin,Lijun Ren,Katy S Papineau,Dustyn A Barnette,Laura K Schnackenberg,Jessica J Hawes,Mark Avigan,Donna L Mendrick,Lorna Ewart,Janey Ronxhi

Drug delivery and translational research 12:1959-1973 PubMed35226290

2022

Therapeutic effects of dexamethasone-loaded hyaluronan nanogels in the experimental cholestasis.

Applications

Unspecified application

Species

Unspecified reactive species

Sabina Di Matteo,Chiara Di Meo,Guido Carpino,Nicole Zoratto,Vincenzo Cardinale,Lorenzo Nevi,Diletta Overi,Daniele Costantini,Claudio Pinto,Elita Montanari,Marco Marzioni,Luca Maroni,Antonio Benedetti,Marco Viola,Tommasina Coviello,Pietro Matricardi,Eugenio Gaudio,Domenico Alvaro

Nature communications 10:2184 PubMed31097716

2019

Ciclopirox inhibits Hepatitis B Virus secretion by blocking capsid assembly.

Applications

Unspecified application

Species

Unspecified reactive species

Jung-Ah Kang,Songwon Kim,Minji Park,Hyun-Jin Park,Jeong-Hyun Kim,Sanghyeok Park,Jeong-Ryul Hwang,Yong-Chul Kim,Yoon Jun Kim,Yuri Cho,Mi Sun Jin,Sung-Gyoo Park
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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