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AB267629

Human AMH ELISA Kit

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(2 Publications)

Human AMH ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human AMH in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

MIF, AMH, Muellerian-inhibiting factor, Anti-Muellerian hormone, Muellerian-inhibiting substance, MIS

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Sandwich ELISA - Human AMH ELISA Kit (AB267629)
  • sELISA

Supplier Data

Sandwich ELISA - Human AMH ELISA Kit (AB267629)

Human AMH ELISA kit (ab267629) Standard curve.

This standard curve is for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture media, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.18 ng/mL

Range

0.164 - 40 ng/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human AMH ELISA Kit is designed for the quantitative determination of AMH in cell culture supernatants, plasma and serum samples.

Δ Note: AMH concentration is low in normal serum/plasma, and may not be detectable in this assay.

This assay employs an antibody specific for human AMH coated on a 96-well plate. Standards and samples are pipetted into the wells and AMH present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human AMH antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of AMH bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Serum", "range": "71 - 144 %", "average": "= 99.9" }, { "sample": "Plasma", "range": "72 - 106 %", "average": "= 84" }, { "sample": "Cell culture media", "range": "97 - 143 %", "average": "= 121" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab267629-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB267629-CMP02", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB267629-CMP06", "productcode":"" }, { "size":"1 x 200 µL", "name":"400X HRP-Streptavidin Concentrate", "number":"AB267629-CMP07", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB267629-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate Solution", "number":"AB267629-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Human AMH coated Microplate (12 x 8 wells)", "number":"AB267629-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Human AMH Detection Antibody", "number":"AB267629-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human AMH Standard (Lyophilized)", "number":"AB267629-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Plays an important role in several reproductive functions. Induces Muellerian duct regression during male fetal sexual differentiation (PubMed : 34155118, PubMed : 3754790, PubMed : 8469238). Also plays a role in Leydig cell differentiation and function (By similarity). In female acts as a negative regulator of the primordial to primary follicle transition and decreases FSH sensitivity of growing follicles (PubMed : 14742691). AMH signals by binding to a specific type-II receptor, AMHR2, that heterodimerizes with type-I receptors (ACVR1 and BMPR1A), and recruiting SMAD proteins that are translocated to the nucleus to regulate target gene expression (PubMed : 20861221, PubMed : 34155118).
See full target information AMH
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Theranostics 11:8894-8908 PubMed34522217

2021

A stem cell-derived ovarian regenerative patch restores ovarian function and rescues fertility in rats with primary ovarian insufficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Sichen Zhang,Dashuai Zhu,Zhenhua Li,Ke Huang,Shiqi Hu,Halle Lutz,Mengjie Xie,Xuan Mei,Junlang Li,Genevieve Neal-Perry,Shaowei Wang,Ke Cheng

American journal of translational research 13:5350-5355 PubMed34150129

2021

Evaluation of diagnosis and prognosis with anti-mullerian hormone level in early missed abortion.

Applications

Unspecified application

Species

Unspecified reactive species

Juan Xie,Xuan Zhou,Lin Fang,Jun Xiong,Xiaoling Tao
View all publications
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Product promise

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