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AB318930

Human AMH ELISA Kit

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Human AMH ELISA Kit is a single-wash 90-min Simplestep used to quantify Human AMH with a sensitivity of 18.389 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput

View Alternative Names

MIF, AMH, Anti-Muellerian hormone, Muellerian-inhibiting factor, Muellerian-inhibiting substance, MIS

7 Images
Sandwich ELISA - Human AMH ELISA Kit (AB318930)
  • sELISA

Supplier Data

Sandwich ELISA - Human AMH ELISA Kit (AB318930)

Interpolated concentrations of human AMH in human serum in 96-well vs. 384-well plates. Interpolated concentration of native AMH was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 22.5% human serum.  The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS + 1X Enhancer.

Sandwich ELISA - Human AMH ELISA Kit (AB318930)
  • sELISA

Supplier Data

Sandwich ELISA - Human AMH ELISA Kit (AB318930)

Interpolated concentrations of AMH in normal human serum donors. Serum of ten individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean AMH concentration of detected samples was determined to be 2,090 pg/mL with a range of undetected – 6,208 pg/mL.

Sandwich ELISA - Human AMH ELISA Kit (AB318930)
  • sELISA

Supplier Data

Sandwich ELISA - Human AMH ELISA Kit (AB318930)

Interpolated concentrations of AMH in normal human serum donors. Serum of ten individual healthy human male donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean AMH concentration was determined to be 2,698 pg/mL with a range of 998 – 4,674 pg/mL.

Sandwich ELISA - Human AMH ELISA Kit (AB318930)
  • sELISA

Supplier Data

Sandwich ELISA - Human AMH ELISA Kit (AB318930)

Interpolated concentrations of human AMH in serum, plasma (citrate), plasma (EDTA), and plasma (heparin). Interpolated concentration of native AMH was measured in duplicate at different sample concentrations. Undiluted samples are 45% serum, plasma (citrate), plasma (EDTA), and plasma (heparin).  The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS + 1X Enhancer.

Sandwich ELISA - Human AMH ELISA Kit (AB318930)
  • sELISA

Supplier Data

Sandwich ELISA - Human AMH ELISA Kit (AB318930)

Interpolated concentrations of human AMH in monkey serum and rat serum. Interpolated concentration of native AMH was measured in duplicate at different sample concentrations. Undiluted samples are 22.5% rat serum, and 2.25% monkey serum.  The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS + 1X Enhancer.

Sandwich ELISA - Human AMH ELISA Kit (AB318930)
  • sELISA

Supplier Data

Sandwich ELISA - Human AMH ELISA Kit (AB318930)

Example of human AMH standard curve in Sample Diluent NS + 1X Enhancer in 96-well vs. 384-well plate. Example of human AMH standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human AMH ELISA Kit (AB318930)
  • sELISA

Supplier Data

Sandwich ELISA - Human AMH ELISA Kit (AB318930)

Example of human AMH standard curve in Sample Diluent NS + 1X Enhancer. Example of human AMH standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Serum, EDTA Plasma, Cell culture media, Citrate plasma, Heparin Plasma

Reacts with

Human, Rat, Monkey

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 18.389 pg/mL

Range

43.75 - 2800 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human AMH SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of AMH protein in Serum, Cit Plasma, EDTA Plasma, Hep Plasma, Cell culture media. Quantitate Human AMH with 18.389 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
-Also available in a fully validated 384-well format.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.1" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.6" } ]
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Recovery

[ { "sample": "Serum", "range": "87 - 108 %", "average": "= 100" }, { "sample": "Citrate plasma", "range": "100 - 117 %", "average": "= 107" }, { "sample": "EDTA Plasma", "range": "94 - 105 %", "average": "= 99" }, { "sample": "Heparin Plasma", "range": "86 - 105 %", "average": "= 94" }, { "sample": "Cell culture media", "range": "82 - 106 %", "average": "= 98" } ]
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What's included?

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab318930-10x96Tests", "assetComponentDetails": [ { "size":"10 x 1 Unit", "name":"Plate Seals", "number":"AB318930-CMP11" }, { "size":"10 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB318930-CMP05" }, { "size":"1 x 120 mL", "name":"Stop Solution", "number":"AB318930-CMP09" }, { "size":"2 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB318930-CMP07" }, { "size":"1 x 120 mL", "name":"TMB Development Solution", "number":"AB318930-CMP08" }, { "size":"10 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB318930-CMP04" }, { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB318930-CMP06" }, { "size":"20 x 1 Vial", "name":"Human AMH Lyophilized Recombinant Protein", "number":"AB318930-CMP03" }, { "size":"10 x 600 µL", "name":"10X Human AMH Capture Antibody", "number":"AB318930-CMP01" }, { "size":"10 x 600 µL", "name":"10X Human AMH Detector Antibody", "number":"AB318930-CMP02" }, { "size":"10 x 96 Wells", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB318930-CMP10" } ] }, "1x384Tests": { "sellingSize": "1 x 384 Tests", "publicAssetCode":"ab318930-1x384Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB318930-CMP11" }, { "size":"1 x 5 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB318930-CMP05" }, { "size":"2 x 12 mL", "name":"Stop Solution", "number":"AB318930-CMP09" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB318930-CMP07" }, { "size":"2 x 12 mL", "name":"TMB Development Solution", "number":"AB318930-CMP08" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB318930-CMP06" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB318930-CMP04" }, { "size":"1 x 600 µL", "name":"10X Human AMH Capture Antibody", "number":"AB318930-CMP01" }, { "size":"2 x 1 Vial", "name":"Human AMH Lyophilized Recombinant Protein", "number":"AB318930-CMP03" }, { "size":"1 x 600 µL", "name":"10X Human AMH Detector Antibody", "number":"AB318930-CMP02" }, { "size":"1 x 384 Wells", "name":"384 well CaptSure™ microplates", "number":"AB318930-CMP12" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab318930-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB318930-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB318930-CMP09", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB318930-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB318930-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB318930-CMP08", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB318930-CMP06", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human AMH Capture Antibody", "number":"AB318930-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human AMH Lyophilized Recombinant Protein", "number":"AB318930-CMP03", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB318930-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human AMH Detector Antibody", "number":"AB318930-CMP02", "productcode":"" }, { "size":"1 x 96 Wells", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB318930-CMP10", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

The anti-Muellerian hormone (AMH) plays an important role in several reproductive functions (PubMed : 14742691, PubMed : 34155118, PubMed : 3754790, PubMed : 8469238). Anti-Muellerian hormone binds and activates AMHR2, its specific type-II receptor, that heterodimerizes with type-I receptors (ACVR1 and BMPR1A) to regulate target gene expression through downstream SMAD protein signal transduction (PubMed : 20861221, PubMed : 34155118). Produced and secreted by Sertoli cells of the male fetus, anti-Muellerian hormone induces Muellerian duct regression during male fetal sexual differentiation (PubMed : 34155118, PubMed : 3754790, PubMed : 8469238). In female, it is produced by granulosa cells of the preantral and small antral follicles and acts as a negative regulator of the primordial to primary follicle transition and decreases FSH sensitivity of growing follicles (PubMed : 14742691). Also plays a role in Leydig cell differentiation and function (By similarity).
See full target information AMH
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websiteProtocolBooklet
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Product promise

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