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AB326752

Human AMH ELISA Kit, Chemiluminescent

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Human AMH ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Human AMH with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 3.89 - 8500 pg/ml with a sensitivity of 2.85 pg/mL.
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
- Patent pending

View Alternative Names

MIF, AMH, Anti-Muellerian hormone, Muellerian-inhibiting factor, Muellerian-inhibiting substance, MIS

5 Images
Sandwich ELISA - Human AMH ELISA Kit, Chemiluminescent (AB326752)
  • sELISA

Supplier Data

Sandwich ELISA - Human AMH ELISA Kit, Chemiluminescent (AB326752)

Interpolated concentrations of human AMH in serum, plasma (citrate), plasma (EDTA), and plasma (heparin). Interpolated concentration of native AMH was measured in duplicate at different sample concentrations. Undiluted samples are 45% serum, plasma (citrate), plasma (EDTA), and plasma (heparin).  The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS + 1X Enhancer.

Sandwich ELISA - Human AMH ELISA Kit, Chemiluminescent (AB326752)
  • sELISA

Supplier Data

Sandwich ELISA - Human AMH ELISA Kit, Chemiluminescent (AB326752)

Interpolated concentrations of AMH in normal human serum donors. Serum of ten individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean AMH concentration of detected samples was determined to be 2,090 pg/mL with a range of undetected – 6,208 pg/mL.

Sandwich ELISA - Human AMH ELISA Kit, Chemiluminescent (AB326752)
  • sELISA

Supplier Data

Sandwich ELISA - Human AMH ELISA Kit, Chemiluminescent (AB326752)

Interpolated concentrations of AMH in normal human serum donors. Serum of ten individual healthy human male donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean AMH concentration was determined to be 2,698 pg/mL with a range of 998 – 4,674 pg/mL.

Sandwich ELISA - Human AMH ELISA Kit, Chemiluminescent (AB326752)
  • sELISA

Supplier Data

Sandwich ELISA - Human AMH ELISA Kit, Chemiluminescent (AB326752)

Interpolated concentrations of human AMH in monkey serum and rat serum. Interpolated concentration of native AMH was measured in duplicate at different sample concentrations. Undiluted samples are 22.5% rat serum, and 2.25% monkey serum.  The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS + 1X Enhancer.

Sandwich ELISA - Human AMH ELISA Kit, Chemiluminescent (AB326752)
  • sELISA

Lab

Sandwich ELISA - Human AMH ELISA Kit, Chemiluminescent (AB326752)

Standard curve comparison between the Chemiluminescent Human AMH SimpleStep ELISA and colorimetric Human AMH SimpleStep ELISA (ab318930) in Sample Diluent NS + 1X Enhancer. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Luminescent

Sample types

Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture media

Reacts with

Human, Rat, Monkey

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 2.85 pg/mL

Range

3.89 - 8500 pg/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

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Product details

Human AMH ELISA Kit, Chemiluminescent (ab326752) is a rapid single-wash sandwich 55-min ELISA to measure Human AMH in Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture media with a sensitivity of 2.85 pg/mL.

How the assay works

Human AMH ELISA Kit, Chemiluminescent (ab326752) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human AMH (ab326752) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-AMH antibody [EPR25835-80] - BSA and Aside free (Capture) ab314564
- Anti-AMH antibody [EPR25767-77] - BSA and Aside free (Detector) ab314565
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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 6.1" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 10.6" } ]
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Recovery

[ { "sample": "Serum", "range": "87 - 108 %", "average": "= 108" }, { "sample": "Citrate plasma", "range": "100 - 117 %", "average": "= 117" }, { "sample": "EDTA Plasma", "range": "94 - 105 %", "average": "= 105" }, { "sample": "Heparin Plasma", "range": "86 - 105 %", "average": "= 105" }, { "sample": "Cell culture media", "range": "82 - 106 %", "average": "= 106" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

The anti-Muellerian hormone (AMH) plays an important role in several reproductive functions (PubMed : 14742691, PubMed : 34155118, PubMed : 3754790, PubMed : 8469238). Anti-Muellerian hormone binds and activates AMHR2, its specific type-II receptor, that heterodimerizes with type-I receptors (ACVR1 and BMPR1A) to regulate target gene expression through downstream SMAD protein signal transduction (PubMed : 20861221, PubMed : 34155118). Produced and secreted by Sertoli cells of the male fetus, anti-Muellerian hormone induces Muellerian duct regression during male fetal sexual differentiation (PubMed : 34155118, PubMed : 3754790, PubMed : 8469238). In female, it is produced by granulosa cells of the preantral and small antral follicles and acts as a negative regulator of the primordial to primary follicle transition and decreases FSH sensitivity of growing follicles (PubMed : 14742691). Also plays a role in Leydig cell differentiation and function (By similarity).
See full target information AMH
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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