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AB267597

Human AMICA ELISA Kit (JAML)

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Human AMICA ELISA Kit (JAML) is a Sandwich (quantitative) ELISA for the measurement of Human AMICA (JAML) in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

AMICA1, UNQ722/PRO1387, JAML, Junctional adhesion molecule-like, Adhesion molecule interacting with CXADR antigen 1, Dendritic cell-specific protein CREA7-1

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Sandwich ELISA - Human AMICA ELISA Kit (JAML) (AB267597)
  • sELISA

Unknown

Sandwich ELISA - Human AMICA ELISA Kit (JAML) (AB267597)

This standard curve is for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.41 ng/mL

Range

0.41 - 100 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human AMICA ELISA Kit is designed for the quantitative determination of AMICA in cell culture supernatants, plasma and serum samples.

This assay employs an antibody specific for human AMICA coated on a 96-well plate. Standards and samples are pipetted into the wells and AMICA present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human AMICA antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of AMICA bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

This ELISA kit shows no cross-reactivity with the following cytokines tested: human aFGF, Angiopoietin-4, BAFF, CD163, Clusterin, CRTAM, CXCL14, Cystatin C, DLL1, Fetuin A, FOLR1, GASP-1, GASP-2, GCSF R, HAI-2, IL-17 RB, IL-27, LAG-3, LDL R, Pepsinogen 1, RBP4, SOST, Syndecan-1, TACI, TFPI, Thrombospondin 1, TRANCE, Troponin I, uPA, WISP-1.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Serum", "range": "97 - 137 %", "average": "= 116.3" }, { "sample": "Plasma", "range": "87 - 133 %", "average": "= 111.3" }, { "sample": "Cell culture media", "range": "86 - 93 %", "average": "= 88.7" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab267597-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB267597-CMP02", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB267597-CMP06", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB267597-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate Solution", "number":"AB267597-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human AMICA Standard (Lyophilized)", "number":"AB267597-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Human AMICA Detection Antibody", "number":"AB267597-CMP04", "productcode":"" }, { "size":"1 x 200 µL", "name":"400X HRP-Streptavidin Concentrate", "number":"AB267597-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Human AMICA coated Microplate (12 x 8 wells)", "number":"AB267597-CMP08", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein JAML also referred to as Junctional Adhesion Molecule-Like is an important transmembrane protein with a molecular mass of approximately 36 kDa. It belongs to the immunoglobulin superfamily and functions in the immune response. JAML is widely expressed in epithelial cells and various immune cells particularly in neutrophils and some subsets of T cells. On a cellular level JAML is involved in cell-cell adhesion processes functioning at interfaces where immune cells interact with epithelial layers.
Biological function summary

Beyond serving as a simple structural component JAML establishes interactions critical for immune surveillance and cell movement. It pairs with other immune receptors to promote the transmigration of leukocytes across endothelial and epithelial barriers. This interaction is notably important since JAML mediates adhesion through its engagement with the Coxsackievirus and Adenovirus Receptor (CAR) forming dynamic complexes that facilitate immune cell trafficking.

Pathways

Many cellular processes engage JAML in key signaling pathways responsible for immune responses. The protein takes part in the integrin signaling pathway affecting cellular adhesion and migration. Additionally JAML interacts with LFA-1 (Lymphocyte Function-associated Antigen 1) an integrin involved in leukocyte function modulating pathways that govern immune cell behavior and inflammation responses.

The functional role of JAML becomes particularly significant in contexts of inflammatory conditions and cancer. Inflammatory diseases such as inflammatory bowel disease (IBD) show considerable involvement of JAML as its regulatory influence on leukocyte transmigration exacerbates inflammation. Similarly changes in JAML expression have implications in cancer metastasis mechanisms as they relate to immune evasion in tumors. In these conditions JAML interacts closely with proteins like CAR and LFA-1 linking its expression and activity directly to disease progression and immune response modulation.
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Product protocols

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Target data

Transmembrane protein of the plasma membrane of leukocytes that control their migration and activation through interaction with CXADR, a plasma membrane receptor found on adjacent epithelial and endothelial cells. The interaction between both receptors mediates the activation of gamma-delta T-cells, a subpopulation of T-cells residing in epithelia and involved in tissue homeostasis and repair. Upon epithelial CXADR-binding, JAML induces downstream cell signaling events in gamma-delta T-cells through PI3-kinase and MAP kinases. It results in proliferation and production of cytokines and growth factors by T-cells that in turn stimulate epithelial tissues repair. It also controls the transmigration of leukocytes within epithelial and endothelial tissues through adhesive interactions with epithelial and endothelial CXADR.
See full target information JAML
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