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AB277417

Human AMN ELISA kit

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Human AMN ELISA kit is a Sandwich ELISA for the measurement of Human AMN in Human in Cell Culture Media, Biofluids samples.

View Alternative Names

UNQ513/PRO1028, AMN, Protein amnionless

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ELISA - Human AMN ELISA kit (AB277417)
  • ELISA

Supplier Data

ELISA - Human AMN ELISA kit (AB277417)

These standard curves are for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Citrate plasma, EDTA Plasma, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 21 pg/mL

Range

20.5 - 5000 pg/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Primary Antibodies

AB224213

Anti-AMN antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMN antibody (AB224213)

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Proteins & Peptides

AB139248

Recombinant Human AMN protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human AMN protein (His tag N-Terminus) (AB139248)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human AMN ELISA Kit (ab277417) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human AMN in serum, plasma (collect plasma using EDTA and Citrate as an anticoagulant. Heparin is not recommended), and cell culture supernatants.

This assay employs an antibody specific for Human AMN coated on a 96-well plate. Standards and samples are pipetted into the wells and Human AMN present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human AMN antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human AMN bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" } ]
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Recovery

[ { "sample": "Serum", "range": "87 - 145 %", "average": "= 116.6" }, { "sample": "Cell culture media", "range": "92 - 147 %", "average": "= 125.4" }, { "sample": "Plasma", "range": "70 - 89 %", "average": "= 75.71" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab277417-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB277417-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB277417-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Human AMN coated Microplate", "number":"AB277417-CMP02", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB277417-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human AMN standard protein (Lyophilized)", "number":"AB277417-CMP04", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin Concentrate", "number":"AB277417-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Human AMN Antibody", "number":"AB277417-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB277417-CMP07", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Membrane-bound component of the endocytic receptor formed by AMN and CUBN (PubMed : 14576052, PubMed : 29402915, PubMed : 30523278). Required for normal CUBN glycosylation and trafficking to the cell surface (PubMed : 14576052, PubMed : 29402915). The complex formed by AMN and CUBN is required for efficient absorption of vitamin B12 (PubMed : 12590260, PubMed : 14576052, PubMed : 26040326). Required for normal CUBN-mediated protein transport in the kidney (Probable).
See full target information AMN
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websiteProtocolBooklet
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Product promise

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