Human AMN ELISA kit is a Sandwich (quantitative) ELISA kit for the measurement of Human AMN in Human in Cell culture supernatant, Citrate plasma, EDTA Plasma, Serum samples.
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Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture supernatant, Citrate plasma, EDTA Plasma, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 20.5 - 5000 pg/mL
- Sensitivity
- = 21 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Membrane-bound component of the endocytic receptor formed by AMN and CUBN (PubMed:14576052, PubMed:29402915, PubMed:30523278). Required for normal CUBN glycosylation and trafficking to the cell surface (PubMed:14576052, PubMed:29402915). The complex formed by AMN and CUBN is required for efficient absorption of vitamin B12 (PubMed:12590260, PubMed:14576052, PubMed:26040326). Required for normal CUBN-mediated protein transport in the kidney (Probable).
Alternative names
UNQ513/PRO1028, AMN, Protein amnionless
What's included?
Recommended products
Human AMN ELISA kit is a Sandwich (quantitative) ELISA kit for the measurement of Human AMN in Human in Cell culture supernatant, Citrate plasma, EDTA Plasma, Serum samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture supernatant, Citrate plasma, EDTA Plasma, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 20.5 - 5000 pg/mL
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 21 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. < 10 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. < 12 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 116.6 | Range 87 - 145 % |
Sample type Cell culture media | Average % = 125.4 | Range 92 - 147 % |
Sample type Plasma | Average % = 75.71 | Range 70 - 89 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Human AMN ELISA Kit (ab277417) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human AMN in serum, plasma (collect plasma using EDTA and Citrate as an anticoagulant. Heparin is not recommended), and cell culture supernatants.
This assay employs an antibody specific for Human AMN coated on a 96-well plate. Standards and samples are pipetted into the wells and Human AMN present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human AMN antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human AMN bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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1 product image
ELISA - Human AMN ELISA kit (ab277417)
These standard curves are for demonstration only. A standard curve must be run with each assay.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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