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AB99975

Human Amphiregulin ELISA Kit (AREG)

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(6 Publications)

Human Amphiregulin ELISA Kit (AREG) is a Sandwich (quantitative) ELISA for the measurement of Human Amphiregulin (AREG) in Human in Biofluids, Cell Culture Media samples.
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Sandwich ELISA - Human Amphiregulin ELISA Kit (AREG) (AB99975)
  • sELISA

Supplier Data

Sandwich ELISA - Human Amphiregulin ELISA Kit (AREG) (AB99975)

Representative Standard Curve using ab99975.

Sandwich ELISA - Human Amphiregulin ELISA Kit (AREG) (AB99975)
  • sELISA

Supplier Data

Sandwich ELISA - Human Amphiregulin ELISA Kit (AREG) (AB99975)

Representative Standard Curve using ab99975.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

< 10 pg/mL

Range

16.46 - 4000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's Amphiregulin (AREG) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Amphiregulin in plasma, and cell culture supernatants. (Human Amphiregulin concentration is pretty low in normal plasma, it may not be detected in this assay).

This assay employs an antibody specific for Human Amphiregulin coated on a 96-well plate. Standards and samples are pipetted into the wells and Amphiregulin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Amphiregulin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Amphiregulin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

We have not been able to detect endogenous Human Amphiregulin in normal serum with ab99975, only in serum spiked with Human Amphiregulin.

Optimisation may be required with urine samples.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "89 - 111 %", "average": "= 100.4" }, { "sample": "Plasma", "range": "64 - 87 %", "average": "= 72.7" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab99975-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB99975-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB99975-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Human Amphiregulin Standard (lyophilized)", "number":"AB99975-CMP03", "productcode":"" }, { "size":"1 x 200 µL", "name":"500X HRP-Streptavidin Concentrate", "number":"AB99975-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Human Amphiregulin", "number":"AB99975-CMP09", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB99975-CMP06", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB99975-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB99975-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Amphiregulin Microplate (12 x 8 well strips)", "number":"AB99975-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

'Amphiregulin' also known as 'AREG' or 'Amphiregulin protein' is a member of the epidermal growth factor (EGF) family. It is a 252 amino acid protein with a predicted mass of approximately 27-29 kDa. Amphiregulin shows expression in various tissues including epithelial cells fibroblasts and immune cells. This protein functions as a ligand for the EGF receptor (EGFR) leading to receptor dimerization and subsequent activation of downstream signaling pathways.
Biological function summary

'Amphiregulin' plays a critical role in cell proliferation differentiation and tissue remodeling. It is not part of a constitutive complex but rather exerts its effects through interaction with EGFR a receptor tyrosine kinase. Amphiregulin contributes significantly to mammary gland development and has a notable role in wound healing. Beyond its developmental roles Amphiregulin can impact immune modulation and inflammation processes.

Pathways

'Amphiregulin' significantly contributes to the mitogen-activated protein kinase (MAPK) and phosphoinositide 3-kinase (PI3K) pathways. These pathways influence cell survival growth and proliferation. Amphiregulin interacts with EGFR and by extension has relationships with other EGFR ligands such as EGF and transforming growth factor-alpha (TGF-alpha). These pathways underline the importance of Amphiregulin in maintaining cellular functions and responding to external stimuli.

'Amphiregulin' has strong associations with cancer and asthma. Overexpression of Amphiregulin can lead to increased cancer cell proliferation and it has been implicated in diverse cancers such as breast and colorectal cancer. Additionally the protein plays a role in airway remodeling in asthma contributing to bronchial hyper-responsiveness. In these contexts Amphiregulin's interactions with EGFR are central influencing disease progression and response to treatment.
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Product protocols

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Target data

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 11:5799 PubMed33199705

2020

Transient commensal clonal interactions can drive tumor metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Suha Naffar-Abu Amara,Hendrik J Kuiken,Laura M Selfors,Timothy Butler,Marco L Leung,Cheuk T Leung,Elaine P Kuhn,Teodora Kolarova,Carina Hage,Kripa Ganesh,Richard Panayiotou,Rosemary Foster,Bo R Rueda,Athena Aktipis,Paul Spellman,Tan A Ince,Joanne Xiu,Matthew Oberley,Zoran Gatalica,Nicholas Navin,Gordon B Mills,Rodrick T Bronson,Joan S Brugge

Inflammation 44:558-569 PubMed33098052

2020

Imperatorin Relieved Ulcerative Colitis by Regulating the Nrf-2/ARE/HO-1 Pathway in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Min Luo,Yin Luo

International journal of oncology 53:2433-2444 PubMed30272278

2018

miR-103 promotes the metastasis and EMT of hepatocellular carcinoma by directly inhibiting LATS2.

Applications

Unspecified application

Species

Unspecified reactive species

Li-Li Han,Xiao-Ran Yin,Shu-Qun Zhang

Cell 168:1101-1113.e13 PubMed28283064

2017

Complement Component 3 Adapts the Cerebrospinal Fluid for Leptomeningeal Metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Adrienne Boire,Yilong Zou,Jason Shieh,Danilo G Macalinao,Elena Pentsova,Joan Massagué

Oncotarget 6:22348-60 PubMed26084293

2015

ERBB activation modulates sensitivity to MEK1/2 inhibition in a subset of driver-negative melanoma.

Applications

Unspecified application

Species

Unspecified reactive species

Katherine E Hutchinson,Douglas B Johnson,Adam S Johnson,Violeta Sanchez,Maria Kuba,Pengcheng Lu,Xi Chen,Mark C Kelley,Qingguo Wang,Zhongming Zhao,Mark Kris,Michael F Berger,Jeffrey A Sosman,William Pao

Molecular cancer therapeutics 14:769-78 PubMed25713333

2015

Multiple molecular subtypes of triple-negative breast cancer critically rely on androgen receptor and respond to enzalutamide in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Valerie N Barton,Nicholas C D'Amato,Michael A Gordon,Hanne T Lind,Nicole S Spoelstra,Beatrice L Babbs,Richard E Heinz,Anthony Elias,Paul Jedlicka,Britta M Jacobsen,Jennifer K Richer
View all publications
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