AB325075

Human Amyloid-beta 1-38 ELISA Kit

SimpleStep
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Human Amyloid-beta 1-38 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Amyloid-beta 1-38 with a sensitivity of 8.44pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

-Colormetric sandwich ELISA - 450 nm readout- works on any standard plate reader.

-Different formats for different needs: 10 x 96 plates for bulk orders and 384-well for higher throughput.

-Validated on a number of sample types including cerebrospinal fluid (CSF)

View Alternative Names

A4, AD1, App, Amyloid-beta precursor protein, ABPP, Alzheimer disease amyloid A4 protein homolog, Alzheimer disease amyloid protein, Amyloid precursor protein, Amyloid-beta (A4) precursor protein, Amyloid-beta A4 protein, Amyloidogenic glycoprotein, APP, AG

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Sandwich ELISA - Human Amyloid-beta 1-38 ELISA Kit (AB325075)

sELISA

Lab

Sandwich ELISA - Human Amyloid-beta 1-38 ELISA Kit (AB325075)

Interpolated concentration of native Amyloid Beta 1-38 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 10% human cerebrospinal fluid. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Lab

Sandwich ELISA - Human Amyloid-beta 1-38 ELISA Kit (AB325075)

Example of human Amyloid Beta 1-38 standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Lab

Sandwich ELISA - Human Amyloid-beta 1-38 ELISA Kit (AB325075)

Interpolated concentration of native Amyloid Beta 1-38 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : human cerebrospinal fluid (CSF) 10%, mouse CSF 20%, and rat CSF 50%. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Lab

Sandwich ELISA - Human Amyloid-beta 1-38 ELISA Kit (AB325075)

Example of human Amyloid Beta 1-38 standard curve in 96-well vs. 384-well plate in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture media, Cerebral Spinal Fluid

Reacts with

Human, Mouse, Rat

Assay type

Sandwich (quantitative)

Sensitivity

= 8.44 pg/mL

Range

25 - 400 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human Amyloid-beta 1-38 ELISA Kit (ab325075) is a rapid single-wash sandwich 90-min ELISA to measure Human Amyloid-beta 1-38 in Cell Culture Media, Cerebrospinal Fluid. This SimpleStep sensitivity is 8.44 pg/ml.

How the assay works

Human Amyloid-beta 1-38 ELISA Kit (ab325075) SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human Amyloid-beta 1-38 (ab325075) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Save your precious samples

Our Human Amyloid-beta 1-38 ELISA Kit only requires 12.5µL sample.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Cerebral Spinal Fluid", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 3.1" }, { "reproducibilityType": "Inter", "sample": "Cerebral Spinal Fluid", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 3.8" } ]

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Recovery

[ { "sample": "Cell culture media", "range": "87 - 94 %", "average": "= 91" }, { "sample": "Cerebral Spinal Fluid", "range": "95 - 105 %", "average": "= 99" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

Functions as a cell surface receptor and performs physiological functions on the surface of neurons relevant to neurite growth, neuronal adhesion and axonogenesis. Interaction between APP molecules on neighboring cells promotes synaptogenesis. Involved in cell mobility and transcription regulation through protein-protein interactions. Can promote transcription activation through binding to APBB1-KAT5 and inhibit Notch signaling through interaction with Numb. Couples to apoptosis-inducing pathways such as those mediated by G(o) and JIP. Inhibits G(o)-alpha ATPase activity (By similarity). Acts as a kinesin I membrane receptor, mediating the axonal transport of beta-secretase and presenilin 1 (By similarity). By acting as a kinesin I membrane receptor, plays a role in axonal anterograde transport of cargo towards synapses in axons (By similarity). May be involved in copper homeostasis/oxidative stress through copper ion reduction. Can regulate neurite outgrowth through binding to components of the extracellular matrix such as heparin and collagen I and IV (By similarity). The splice isoforms that contain the BPTI domain possess protease inhibitor activity. Induces a AGER-dependent pathway that involves activation of p38 MAPK, resulting in internalization of amyloid-beta peptide and leading to mitochondrial dysfunction in cultured cortical neurons (By similarity). Provides Cu(2+) ions for GPC1 which are required for release of nitric oxide (NO) and subsequent degradation of the heparan sulfate chains on GPC1.. Amyloid-beta peptides are lipophilic metal chelators with metal-reducing activity. Binds transient metals such as copper, zinc and iron. Rat and mouse amyloid-beta peptides bind only weakly transient metals and have little reducing activity due to substitutions of transient metal chelating residues. Amyloid-beta protein 42 may activate mononuclear phagocytes in the brain and elicit inflammatory responses. Promotes both tau aggregation and TPK II-mediated phosphorylation. Also binds GPC1 in lipid rafts (By similarity).. The gamma-CTF peptides as well as the caspase-cleaved peptides, including C31, are potent enhancers of neuronal apoptosis.

See full target information App

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websiteProtocolBooklet

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