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Human Amyloid beta 1-40 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human Amyloid beta 1-40 in Human in Plasma, Cell culture supernatant, Serum samples.

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Sandwich ELISA - Human Amyloid beta 1-40 ELISA Kit (AB193692), expandable thumbnail

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Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

0.1 - 100 ng/mL

Sensitivity

= 0.1 ng/mL

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Reactivity data

Application

sELISA

Reactivity

Reacts

Dilution info
-
Notes

-

Target data

Function

Functions as a cell surface receptor and performs physiological functions on the surface of neurons relevant to neurite growth, neuronal adhesion and axonogenesis. Interaction between APP molecules on neighboring cells promotes synaptogenesis (PubMed:25122912). Involved in cell mobility and transcription regulation through protein-protein interactions. Can promote transcription activation through binding to APBB1-KAT5 and inhibits Notch signaling through interaction with Numb. Couples to apoptosis-inducing pathways such as those mediated by G(o) and JIP. Inhibits G(o) alpha ATPase activity (By similarity). Acts as a kinesin I membrane receptor, mediating the axonal transport of beta-secretase and presenilin 1 (By similarity). By acting as a kinesin I membrane receptor, plays a role in axonal anterograde transport of cargo towards synapses in axons (PubMed:17062754, PubMed:23011729). Involved in copper homeostasis/oxidative stress through copper ion reduction. In vitro, copper-metallated APP induces neuronal death directly or is potentiated through Cu(2+)-mediated low-density lipoprotein oxidation. Can regulate neurite outgrowth through binding to components of the extracellular matrix such as heparin and collagen I and IV. The splice isoforms that contain the BPTI domain possess protease inhibitor activity. Induces a AGER-dependent pathway that involves activation of p38 MAPK, resulting in internalization of amyloid-beta peptide and leading to mitochondrial dysfunction in cultured cortical neurons. Provides Cu(2+) ions for GPC1 which are required for release of nitric oxide (NO) and subsequent degradation of the heparan sulfate chains on GPC1.Amyloid-beta peptides are lipophilic metal chelators with metal-reducing activity. Bind transient metals such as copper, zinc and iron. In vitro, can reduce Cu(2+) and Fe(3+) to Cu(+) and Fe(2+), respectively. Amyloid-beta protein 42 is a more effective reductant than amyloid-beta protein 40. Amyloid-beta peptides bind to lipoproteins and apolipoproteins E and J in the CSF and to HDL particles in plasma, inhibiting metal-catalyzed oxidation of lipoproteins. APP42-beta may activate mononuclear phagocytes in the brain and elicit inflammatory responses. Promotes both tau aggregation and TPK II-mediated phosphorylation. Interaction with overexpressed HADH2 leads to oxidative stress and neurotoxicity. Also binds GPC1 in lipid rafts.Appicans elicit adhesion of neural cells to the extracellular matrix and may regulate neurite outgrowth in the brain.The gamma-CTF peptides as well as the caspase-cleaved peptides, including C31, are potent enhancers of neuronal apoptosis.

Alternative names

What's included?

1 x 96 Tests
Components
1100X HRP-Streptavidin Concentrate
1 x 200 µL
20X Wash Buffer
1 x 25 mL
5X Assay Diluent
1 x 15 mL
Biotinylated Human Amyloid beta detection antibody
2 x 1 Vial
Human Amyloid beta Standards (lyophilized)
2 x 1 Vial
Pre-coated Human amyloid beta 1-40 microplate (12 strips x 8 wells)
1 x 1 Unit
Stop Solution
1 x 8 mL
TMB One-Step Substrate Reagent
1 x 12 mL

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Human Amyloid beta 1-40 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human Amyloid beta 1-40 in Human in Plasma, Cell culture supernatant, Serum samples.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

0.1 - 100 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

Sensitivity

= 0.1 ng/mL

Precision

Intra assay

Sample

Overall

n
0
mean
-
SD
-
C.V.
< 10

Inter assay

Sample

Overall

n
0
mean
-
SD
-
C.V.
< 12

Recovery

Sample specific recovery

Sample type

Serum

Average %

= 101.3

Range

77 - 125 %

Sample type

Plasma

Average %

= 97.39

Range

68 - 136 %

Sample type

Cell culture media

Average %

= 104.3

Range

71 - 145 %

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Notes

Abcam's Amyloid beta 1-40 Human ELISA Kit (ab193692) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Amyloid beta 1-40 in serum, plasma, and cell culture supernatants. (UniprotID: P05067)

This assay employs an antibody specific for Human Amyloid beta 1-40 coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures Amyloid beta 1-40 present in the samples. The wells are washed and biotinylated anti-Human Amyloid beta 1-40 antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of Amyloid beta 1-40 bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Amyloid beta 1-40 often referred to as beta-amyloid or Aβ40 is a peptide with a mass of approximately 4 kDa. It consists of 40 amino acids and constitutes a fragment cleaved from the amyloid precursor protein (APP). This peptide is expressed in various tissues primarily in the brain. Aβ40 is produced through enzymatic processing by β- and γ-secretases with β-APP40 ELISA kits commonly used to measure its levels in research.

Biological function summary

Amyloid beta 1-40 plays a role in cellular processes related to neuronal function and synaptic regulation. It does not act alone but often associates with other molecules forming oligomeric complexes. These complexes can influence membrane permeability and ionic homeostasis in neuronal cells. The peptide is known to disrupt cellular communication and in some contexts can lead to cellular toxicity when overproduced or misregulated.

Pathways

The amyloid beta 1-40 peptide is integral to the amyloidogenic pathway which is part of broader protein processing pathways in the brain. In this pathway it is closely tied to APP and interacts with presenilins which are components of the γ-secretase complex. Another significant pathway is linked to its cytotoxicity effects; this involves Tau protein where Aβ40 can influence hyperphosphorylation of Tau further impacting neurodegenerative processes.

Associated diseases and disorders

Amyloid beta 1-40 is critically involved in Alzheimer's disease and is a hallmark of Alzheimer's pathology due to its aggregation into plaques. The peptide's accumulation correlates with neurodegenerative changes. Besides Alzheimer's Aβ40 has links to cerebral amyloid angiopathy a condition involving amyloid deposition in the walls of the brain's blood vessels. These diseases involve interactions between Aβ40 Tau and APOE proteins which are associated with disease progression and severity.

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1 product image

  • Sandwich ELISA - Human Amyloid beta 1-40 ELISA Kit (ab193692), expandable thumbnail

    Sandwich ELISA - Human Amyloid beta 1-40 ELISA Kit (ab193692)

    Standard Curve using ab193692 Amyloid beta 1-40 Human ELISA Kit.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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