Human Amyloid Precursor Protein ELISA Kit, Chemiluminescent

• sELISA

Lab

Sandwich ELISA - Human Amyloid Precursor Protein ELISA Kit, Chemiluminescent (AB326890)

Dynamic range comparison between the Chemiluminescent SimpleStep ELISA, colorimetric SimpleStep ELISA (ab216944), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and dynamic range.

• sELISA

Lab

Sandwich ELISA - Human Amyloid Precursor Protein ELISA Kit, Chemiluminescent (AB326890)

Standard curve comparison between the Chemiluminescent Human Amyloid Precursor Protein SimpleStep ELISA and colorimetric Human Amyloid Precursor Protein SimpleStep ELISA (ab216944) in Sample Diluent NS.

• sELISA

Supplier Data

Sandwich ELISA - Human Amyloid Precursor Protein ELISA Kit, Chemiluminescent (AB326890)

Interpolated concentrations of native Amyloid Precursor Protein in human extract samples.

The concentrations of Amyloid Precursor Protein were measured in three different dilutions in duplicate and interpolated from the Amyloid Precursor Protein standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted in ng of Amyloid Precursor Protein per mg of extract (mean +/- SD, n=3). Amyloid Precursor Protein concentration was determined to be 213 ng/mg brain tissue extract, 8.24 ng/mg in liver tissue extract, 59.4 ng/mg in SH-SY5Y cell extract, 56.4 ng/mg in U-87 MG cell extract and 50.7 ng/mg in HeLa cell extract samples.

• sELISA

Supplier Data

Sandwich ELISA - Human Amyloid Precursor Protein ELISA Kit, Chemiluminescent (AB326890)

Interpolated concentrations of native Amyloid Precursor Protein in human cerebrospinal fluid (CSF), urine, A-549 cell culture supernatant, and HeLa cell culture supernatant (3 days).

The concentrations of Amyloid Precursor Protein were measured in duplicates, interpolated from the Amyloid Precursor Protein standard curves and corrected for sample dilution. Undiluted samples are as follows : A-549 cell culture supernatant 25%, HeLa cell culture supernatant 25%, cerebrospinal fluid 0.5%, and urine 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Amyloid Precursor Protein concentration was determined to be 23,336 pg/mL in neat A-549 cell culture supernatant, 7,133 pg/mL in neat HeLa cell culture supernatant, 58,7958 pg/mL in neat cerebrospinal fluid, and 9,188 pg/mL in neat urine.

• sELISA

Supplier Data

Sandwich ELISA - Human Amyloid Precursor Protein ELISA Kit, Chemiluminescent (AB326890)

Interpolated concentrations of native Amyloid Precursor Protein in human serum and plasma samples.

The concentrations of Amyloid Precursor Protein were measured in duplicates, interpolated from the Amyloid Precursor Protein standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 10%, plasma (citrate) 10%, plasma (heparin) 20% and plasma (EDTA) 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Amyloid Precursor Protein concentration was determined to be 52,942 pg/mL in neat serum, 63,882 pg/mL in neat plasma (citrate), 31,342 pg/mL in neat plasma (heparin), and 36,605 pg/mL in neat plasma (EDTA).

• sELISA

Supplier Data

Sandwich ELISA - Human Amyloid Precursor Protein ELISA Kit, Chemiluminescent (AB326890)

Interpolated concentrations of native Amyloid Precursor Protein in human brain tissue extract, liver tissue extract, SH-SY5Y cell extract, HeLa cell extract and U-87 MG cell extract.

Interpolated concentrations of native Amyloid Precursor Protein in human brain tissue extract based on a 20 μg/mL extract load, liver tissue extract based on a 500 μg/mL extract load, SH-SY5Y cell extract based on a 100 μg/mL extract load, HeLa cell extract based on a 25 μg/mL extract load, and U-87 MG cell extract based on a 100 μg/mL extract load. The concentrations of Amyloid Precursor Protein were measured in duplicate and interpolated from the Amyloid Precursor Protein standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Amyloid Precursor Protein concentration was determined to be 4,242 pg/mL in brain tissue extract, 4,224 pg/mL in liver tissue extract, 6,041 pg/mL in SH-SY5Y cell extract, 1,207 pg/mL in HeLa cell extract, and 5,676 pg/mL in U-87 MG cell extract.

• sELISA

Supplier Data

Sandwich ELISA - Human Amyloid Precursor Protein ELISA Kit, Chemiluminescent (AB326890)

Serum from nine individual healthy human male donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Amyloid Precursor Protein concentration was determined to be 32,874 pg/mL with a range of 22,456 –‑ 42,097 pg/mL.