Human Angiogenin ELISA Kit
- Recombinant
- SimpleStep
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(1 Publication)
Human Angiogenin ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Angiogenin with a sensitivity of 1.06 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
View Alternative Names
RNASE5, ANG, Angiogenin, Ribonuclease 5, RNase 5
- ELISA
Supplier Data
ELISA - Human Angiogenin ELISA Kit (AB219629)
- sELISA
Supplier Data
Sandwich ELISA - Human Angiogenin ELISA Kit (AB219629)
Example of human Angiogenin standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Supplier Data
Sandwich ELISA - Human Angiogenin ELISA Kit (AB219629)
Interpolated concentrations of spiked Angiogenin in RPMI culture media and native Angiogenin in HepG2 cell culture supernatant samples.
The concentrations of Angiogenin were measured in duplicates, interpolated from the Angiogenin standard curves and corrected for sample dilution. Undiluted samples are as follows : RPMI culture media 50% and HepG2 supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Angiogenin concentration was determined to be 527.60 pg/mL in neat, spiked RPMI culture media. The mean Angiogenin concentration was determined to be 945.93 pg/mL in neat, native HepG2 supernatant.
- sELISA
Supplier Data
Sandwich ELISA - Human Angiogenin ELISA Kit (AB219629)
Serum from ten individual healthy human male donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Angiogenin concentration was determined to be 495.29 ng/mL with a range of 214.57 – 824.72 ng/mL.
- sELISA
Supplier Data
Sandwich ELISA - Human Angiogenin ELISA Kit (AB219629)
Interpolated concentrations of native Angiogenin in human serum and plasma samples.
The concentrations of Angiogenin were measured in duplicates, interpolated from the Angiogenin standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 1,000, plasma (citrate) 1 : 1,000, plasma (EDTA) 1 : 1,000, and plasma (heparin) 1 : 1,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Angiogenin concentration was determined to be 342.53 ng/mL in neat serum, 310.16 ng/mL in neat plasma (citrate), 249.90 ng/mL in neat plasma (EDTA), and 329.36 ng/mL in neat plasma (heparin).
- sELISA
Supplier Data
Sandwich ELISA - Human Angiogenin ELISA Kit (AB219629)
Interpolated concentrations of native Angiogenin in HepG2 cell extract sample based on a 62.5 μg/mL extract load.
The concentrations of Angiogenin were measured in duplicate and interpolated from the Angiogenin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Angiogenin concentration was determined to be 304.03 pg/mL in HepG2 cell extract sample.
- sELISA
Supplier Data
Sandwich ELISA - Human Angiogenin ELISA Kit (AB219629)
Example of human Angiogenin standard curve in Sample Diluent 1X Cell Extraction Buffer PTR.
Background-subtracted data values (mean +/- SD) are graphed.
Complementary Products
Reactivity data
Product details
Human Angiogenin ELISA Kit ab219629 is a rapid single-wash 90-min Sandwich ELISA to measure Human Angiogenin in cell culture extracts, cell culture media, cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 1.06 pg/mL.
How the assay works
Human Angiogenin SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Human Angiogenin ELISA Kit ab219629 protocol summary
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:
- Anti-Angiogenin antibody [EPR19893-126] - BSA and Azide free (Capture) ab242681
- Anti-Angiogenin antibody [EPR19893-51] - BSA and Azide free (Detector) ab242926
Angiogenin is a member of the ribonuclease superfamily with approximately 35% amino acid sequence homology with pancreatic RNase. Angiogenin can act to inhibit protein synthesis by functioning as a cytotoxic tRNA-specific RNase.
Cells expressing Angiogenin include vascular endothelial and smooth muscle cells, fibroblasts, colonic epithelium, peripheral blood lymphocytes, lung cell lines, and primary gastrointestinal adenocarcinomas. Basal levels of Angiogenin are also present in normal human serum and plasma. Angiogenin has been reported to serve as protection for motor neurons and it follows that Angiogenin is associated with neurological diseases, specifically, mutations to this gene are linked to Amyotrophic Lateral Sclerosis (ALS). Human and mouse Angiogenin share 74% homology in amino acid sequence.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Precision
Recovery
What's included?
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Angiogenin significantly contributes to the process of angiogenesis the formation of new blood vessels from pre-existing vascular structures. It characterizes acts as both a ribonuclease and a potent angiogenic factor. Although not part of a multi-protein complex angiogenin interacts with endothelial cells to promote their growth and migration. Angiogenin facilitates these cellular behaviors by triggering pathways that enhance cell survival and proliferation essential for healthy tissue development and repair.
Pathways
Angiogenin is intricately involved in the angiogenesis pathway a critical process for both physiological and pathological conditions. It interacts with proteins such as VEGF (vascular endothelial growth factor) which plays an essential role in vascular function. Angiogenin's interaction with these pathways enhances cellular responses to external stimuli maintaining vascular integrity and supporting tissue regeneration under stress or injury conditions.
Product protocols
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- Download websiteProtocolBooklet|en
Target data
Publications (1)
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Integrative cancer therapies 22:15347354221134513 PubMed36859800
2023
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