AB99972

Human Angiopoietin 1 ELISA Kit (ANG1)

Name: Human Angiopoietin 1 ELISA Kit (ANG1) (ab99972) | Abcam
Brand: Abcam Limited
SKU: AB99972
Price: 780 USD
Availability: InStock

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(6 Publications)

Human Angiopoietin 1 ELISA Kit (ANG1) is a sandwich ELISA designed to quantify Human Angiopoietin 1 (ANG1) with a sensitivity of 30 pg/mL.

- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 74 - 18000 pg/mL

View Alternative Names

KIAA0003, ANGPT1, Angiopoietin-1, ANG-1

2 Images

ELISA - Human Angiopoietin 1 ELISA Kit (ANG1) (AB99972)

ELISA

Supplier Data

ELISA - Human Angiopoietin 1 ELISA Kit (ANG1) (AB99972)

TYPICAL STANDARD CURVE - Assay DIluent A – Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

ELISA

Supplier Data

ELISA - Human Angiopoietin 1 ELISA Kit (ANG1) (AB99972)

TYPICAL STANDARD CURVE - Assay Diluent B – Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 30 pg/mL

Range

74 - 18000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human Angiopoietin 1 ELISA Kit (ANG1) ab99972 is a sandwich ELISA to measure Human Angiopoietin 1 (ANG1) in serum, plasma, cell culture supernatant with a sensitivity of 30 pg/ml.

How the assay works

This assay employs an antibody specific for Human Angiopoietin 1 coated on a 96-well plate. Standards and samples are pipetted into the wells and Angiopoietin 1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Angiopoietin 1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Angiopoietin 1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Assay Specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details

Human Angiopoietin 1 ELISA Kit (ANG1) ab99972 protocol summary

1. Add standard or sample to each well used. Incubate at room temperature
2. Wash and add prepared biotinylated antibody to each well. Incubate at room temperature.
3. Wash and add prepared streptavidin solution. Incubate at room temperature.
4. Add TMB Development Solution to each well. Incubate at room temperature.
5. Add Stop Solution to each well. Read at 450nm immediately.

Optimisation may be required with urine samples.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "83 - 105 %", "average": "= 95.25" }, { "sample": "Serum", "range": "81 - 103 %", "average": "= 94.47" }, { "sample": "Plasma", "range": "80 - 102 %", "average": "= 93.93" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab99972-1x96Tests", "assetComponentDetails": [ { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB99972-CMP03", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB99972-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB99972-CMP06", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB99972-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Angiopoietin 1 Microplate (12 x 8 well strips)", "number":"AB99972-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Human Angiopoietin 1", "number":"AB99972-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Angiopoietin 1 Standard Human (lyophilized)", "number":"AB99972-CMP08", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB99972-CMP01", "productcode":"" }, { "size":"1 x 200 µL", "name":"400X HRP-Streptavidin Concentrate", "number":"AB99972-CMP02", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Angiopoietin 1 also known as Ang1 or Angpt1 is a protein important to the vascular system with a molecular mass of around 70 kDa. It is expressed widely in endothelial cells and fibroblasts. Angiopoietin 1 functions mechanically to bind the Tie2 receptor triggering signaling pathways that regulate blood vessel maturation and stability. This interaction is important for the development and maintenance of the blood vessel network.

Biological function summary

Angiopoietin 1 supports the structural integrity of blood vessels by interacting with the Tie2 receptor as part of a signaling complex. It helps prevent vascular leak and promotes endothelial cell survival migration and adhesion. By playing such roles Angiopoietin 1 is fundamental for maintaining vascular homeostasis and facilitating proper blood flow throughout the body. It also influences the immune response by modulating endothelial functions.

Pathways

Angiopoietin 1's involvement is most notable within the PI3K/AKT pathway and the MAPK pathway which are important for signaling cell survival and growth. These pathways work closely with other proteins such as VEGF (Vascular Endothelial Growth Factor) in angiogenesis and vascular remodeling. Angiopoietin 1 interacts with its related pathways to ensure that blood vessels remain functional and adaptive to physiological changes.

Angiopoietin 1 plays significant roles in cardiovascular diseases like atherosclerosis and diabetic vascular complications. Its dysregulation can lead to abnormalities in vascular maintenance and repair. There is also an association between Angiopoietin 1 and proteins like VEGF in cancer as both have roles in tumor angiogenesis. Understanding Angiopoietin 1's function could provide insights into therapeutic strategies for these conditions.

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Product protocols

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Target data

Binds and activates TEK/TIE2 receptor by inducing its dimerization and tyrosine phosphorylation. Plays an important role in the regulation of angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Required for normal angiogenesis and heart development during embryogenesis. After birth, activates or inhibits angiogenesis, depending on the context. Inhibits angiogenesis and promotes vascular stability in quiescent vessels, where endothelial cells have tight contacts. In quiescent vessels, ANGPT1 oligomers recruit TEK to cell-cell contacts, forming complexes with TEK molecules from adjoining cells, and this leads to preferential activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascades. In migrating endothelial cells that lack cell-cell adhesions, ANGT1 recruits TEK to contacts with the extracellular matrix, leading to the formation of focal adhesion complexes, activation of PTK2/FAK and of the downstream kinases MAPK1/ERK2 and MAPK3/ERK1, and ultimately to the stimulation of sprouting angiogenesis. Mediates blood vessel maturation/stability. Implicated in endothelial developmental processes later and distinct from that of VEGF. Appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme.

See full target information ANGPT1

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Journal of cannabis research 6:43 PubMed39707578

2024

Evaluation of the metabolic activity, angiogenic impacts, and GSK-3β signaling of the synthetic cannabinoid MMB-2201 on human cerebral microvascular endothelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Laith Naser Al-Eitan,Saif Zuhair Alahmad,Sufyan Ali Ajeen,Ahmad Younis Altawil,Iliya Yacoub Khair,Hana Salah Abu Kharmah,Mansour Abdullah Alghamdi

Cells 10: PubMed34831134

2021

Additive Manufacturing of Caffeic Acid-Inspired Mineral Trioxide Aggregate/Poly-ε-Caprolactone Scaffold for Regulating Vascular Induction and Osteogenic Regeneration of Dental Pulp Stem Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ni Tien,Jian-Jr Lee,Alvin Kai-Xing Lee,Yen-Hong Lin,Jian-Xun Chen,Ting-You Kuo,Ming-You Shie

Stem cells translational medicine 9:1389-1400 PubMed32543083

2020

CD34+CD146+ adipose-derived stromal cells enhance engraftment of transplanted fat.

Applications

Unspecified application

Species

Unspecified reactive species

Mimi R Borrelli,Ronak A Patel,Charles Blackshear,Stephanie Vistnes,Nestor M Diaz Deleon,Sandeep Adem,Abra H Shen,Jan Sokol,Arash Momeni,Dung Nguyen,Michael T Longaker,Derrick C Wan

Stem cell research & therapy 11:183 PubMed32430063

2020

Comparative separation methods and biological characteristics of human placental and umbilical cord mesenchymal stem cells in serum-free culture conditions.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao Yi,Feng Chen,Fenghua Liu,Qing Peng,Yang Li,Shao Li,Jiang Du,Yi Gao,Yifeng Wang

Bioscience reports 39: PubMed31138757

2019

Low concentration of rutin treatment might alleviate the cardiotoxicity effect of pirarubicin on cardiomyocytes via activation of PI3K/AKT/mTOR signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Junjie Fei,Yi Sun,Yuyin Duan,Jianming Xia,Songhua Yu,Peigang Ouyang,Teng Wang,Guimin Zhang

PloS one 9:e104712 PubMed25116206

2014

Collagen can selectively trigger a platelet secretory phenotype via glycoprotein VI.

Applications

Unspecified application

Species

Unspecified reactive species

Véronique Ollivier,Varouna Syvannarath,Angèle Gros,Amena Butt,Stéphane Loyau,Martine Jandrot-Perrus,Benoît Ho-Tin-Noé

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human Angiopoietin 1 ELISA Kit (ANG1)

ELISA - Human Angiopoietin 1 ELISA Kit (ANG1) (AB99972)

ELISA - Human Angiopoietin 1 ELISA Kit (ANG1) (AB99972)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay Platform

Complementary Products

Assay Kits

Assay Kits

Assay Kits

Assay Kits

Reactivity data

Product details

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

Product promise