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AB316914

Human Angiopoietin 2/ANG2 ELISA Kit

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Human Angiopoietin 2/ANG2 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Angiopoietin 2/ANG2 with a sensitivity of 73 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader

View Alternative Names

Angiopoietin-2, ANG-2, ANGPT2

3 Images
Sandwich ELISA - Human Angiopoietin 2/ANG2 ELISA Kit (AB316914)
  • sELISA

Supplier Data

Sandwich ELISA - Human Angiopoietin 2/ANG2 ELISA Kit (AB316914)

Interpolated concentration of native Angiopoietin 2/ANG2 was measured in duplicate at different sample concentrations. Undiluted samples are 50% serum, plasma (citrate), plasma (EDTA), plasma (heparin), and HUVEC cell culture supernatant.  The interpolated dilution factor corrected values to neat sample are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human Angiopoietin 2/ANG2 ELISA Kit (AB316914)
  • sELISA

Supplier Data

Sandwich ELISA - Human Angiopoietin 2/ANG2 ELISA Kit (AB316914)

Example of human Angiopoietin 2/ANG2 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Angiopoietin 2/ANG2 ELISA Kit (AB316914)
  • sELISA

Supplier Data

Sandwich ELISA - Human Angiopoietin 2/ANG2 ELISA Kit (AB316914)

Serum of ten individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Angiopoietin 2/ANG2 concentration was determined to be 2,168 pg/mL with a range of 1,269 – 3,011 pg/mL.

Key facts

Detection method

Colorimetric

Sample types

Serum, EDTA Plasma, Citrate plasma, Heparin Plasma, Saliva, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 73 pg/mL

Range

125 - 8000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Angiopoietin 2/ANG2 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Angiopoietin 2/ANG2 protein in Serum, EDTA Plasma, Cit Plasma, Hep Plasma, Saliva, Cell culture supernatant. Quantitate Human Angiopoietin 2/ANG2 with 73 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.2" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5" } ]
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Recovery

[ { "sample": "Serum", "range": "80 - 112 %", "average": "= 96" }, { "sample": "EDTA Plasma", "range": "69 - 94 %", "average": "= 85" }, { "sample": "Citrate plasma", "range": "80 - 107 %", "average": "= 90" }, { "sample": "Heparin Plasma", "range": "65 - 99 %", "average": "= 83" }, { "sample": "Saliva", "range": "89 - 95 %", "average": "= 92" }, { "sample": "Cell culture supernatant", "range": "85 - 97 %", "average": "= 90" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab316914-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB316914-CMP08", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB316914-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB316914-CMP07", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB316914-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Angiopoietin 2/ANG2 Lyophilized Recombinant Protein", "number":"AB316914-CMP03", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB316914-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Angiopoietin 2/ANG2 Capture Antibody", "number":"AB316914-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Angiopoietin 2/ANG2 Detector Antibody", "number":"AB316914-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB316914-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB316914-CMP10", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Angiopoietin-2 also known as ANG2 or ANGPT2 is a glycoprotein with a molecular mass of approximately 70 kDa. It plays an important role in angiogenesis facilitating blood vessel remodeling and maturation. The protein is mainly expressed in endothelial cells where it functions by binding to the Tie2 receptor partially disrupting the Angiopoietin-Tie2 signaling axis. This disruption modulates vascular stability and permeability. The lyophilized form of the ANG-2 protein is often used in research for functional assays like ANG-2 ELISA to quantify its levels in various biological samples.
Biological function summary

Human angiogenesis processes are significantly affected by Angiopoietin-2 which serves as an antagonist to Angiopoietin-1 in regulating vascular dynamics. Although it does not form a stable complex by itself ANG2 activity is closely linked to its interactions with the Tie2 receptor modulating signal pathways that govern endothelial cell survival and permeability. Because of its dual role either stabilizing or destabilizing blood vessels under different conditions ANG2 serves as an important regulator during both normal physiological events and pathophysiological conditions.

Pathways

Angiopoietin-2 significantly contributes to the angiogenesis signaling network. It is deeply involved in the VEGF (Vascular Endothelial Growth Factor) pathway where ANG2 acts to balance VEGF activities in angiogenesis and vessel permeability. The interplay between Angiopoietin-2 and other proteins such as Angiopoietin-1 which stabilizes the vessels is vital for the precise regulation of blood vessel homeostasis during normal and disturbed conditions.

Angiopoietin-2 has a prominent role in the pathology of diseases like cancer and diabetic retinopathy. In cancer excessive ANG2 expression may lead to increased tumor angiogenesis and metastasis making it a target for cancer therapies. In diabetic retinopathy elevated levels of ANG2 correlate with vascular leakage contributing to disease progression. The relationship between Angiopoietin-1 and Angiopoietin-2 in these conditions emphasizes their collective impact on vascular anomalies associated with these disorders.
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Product protocols

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Target data

Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling (PubMed : 15284220, PubMed : 19116766, PubMed : 19223473, PubMed : 9204896). Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1 (PubMed : 15284220, PubMed : 19116766, PubMed : 19223473, PubMed : 9204896). In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal (PubMed : 15284220, PubMed : 19116766, PubMed : 19223473, PubMed : 9204896). Involved in the regulation of lymphangiogenesis (PubMed : 32908006).
See full target information ANGPT2
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