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Human Angiopoietin 2/ANG2 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Angiopoietin 2/ANG2 in Serum, EDTA Plasma, Citrate plasma, Heparin Plasma, Saliva, Cell culture supernatant samples.
Alternative names=Angiopoietin-2, ANG-2, ANGPT2
- Detection method
Colorimetric
- Sample types
Serum, EDTA Plasma, Citrate plasma, Heparin Plasma, Saliva, Cell culture supernatant
- Assay type
Sandwich (quantitative)
- Reactive species
Human
- Range
125 - 8000 pg/mL
- Assay time
1h 30m
- Sensitivity
= 73 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Images
Target data
Function
Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling. Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal.
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Human Angiopoietin 2/ANG2 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Angiopoietin 2/ANG2 in Serum, EDTA Plasma, Citrate plasma, Heparin Plasma, Saliva, Cell culture supernatant samples.
Alternative names=Angiopoietin-2, ANG-2, ANGPT2
Key facts
- Detection method
Colorimetric
- Sample types
Serum, EDTA Plasma, Citrate plasma, Heparin Plasma, Saliva, Cell culture supernatant
- Assay type
Sandwich (quantitative)
- Reactive species
Human
- Range
125 - 8000 pg/mL
- Assay time
1h 30m
- Assay Platform
Pre-coated microplate (12 x 8 well strips)
- Sensitivity
= 73 pg/mL
- General recovery
96%
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 8 | mean - | SD - | C.V. 5.2 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. 5 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 96 | Range 80 - 112 % |
Sample type EDTA Plasma | Average % = 85 | Range 69 - 94 % |
Sample type Citrate plasma | Average % = 90 | Range 80 - 107 % |
Sample type Heparin Plasma | Average % = 83 | Range 65 - 99 % |
Sample type Saliva | Average % = 92 | Range 89 - 95 % |
Sample type Cell culture supernatant | Average % = 90 | Range 85 - 97 % |
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
+4°C
Notes
Human Angiopoietin 2/ANG2 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Angiopoietin 2/ANG2 protein in Serum, EDTA Plasma, Cit Plasma, Hep Plasma, Saliva, Cell culture supernatant. Quantitate Human Angiopoietin 2/ANG2 with 73 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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