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AB99971

Human Angiopoietin 2 ELISA Kit (ANG2)

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(5 Publications)

Human Angiopoietin 2 ELISA Kit (ANG2) is a Sandwich (quantitative) ELISA for the measurement of Human Angiopoietin 2 (ANG2) in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

Angiopoietin-2, ANG-2, ANGPT2

1 Images
Sandwich ELISA - Human Angiopoietin 2 ELISA Kit (ANG2) (AB99971)
  • sELISA

Supplier Data

Sandwich ELISA - Human Angiopoietin 2 ELISA Kit (ANG2) (AB99971)

Representative Standard Curve using ab99971.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

< 10 pg/mL

Range

4.12 - 3000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's Angiopoietin 2 (ANG2) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Angiopoietin 2 in serum, plasma and cell culture supernates.

This assay employs an antibody specific for Human Angiopoietin 2 coated on a 96-well plate. Standards and samples are pipetted into the wells and Angiopoietin 2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Angiopoietin 2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Angiopoietin 2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Optimisation may be required with urine samples.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "76 - 92 %", "average": "= 82.1" }, { "sample": "Serum", "range": "82 - 109 %", "average": "= 85.3" }, { "sample": "Plasma", "range": "95 - 116 %", "average": "= 96.9" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab99971-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB99971-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB99971-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Human Angiopoietin 2", "number":"AB99971-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Angiopoietin 2 Microplate (12 x 8 well strip)", "number":"AB99971-CMP06", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB99971-CMP03", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin Concentrate", "number":"AB99971-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Angiopoietin 2 Standard Human (lyophilized)", "number":"AB99971-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB99971-CMP05", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Angiopoietin-2 also known as ANG2 or ANGPT2 is a glycoprotein with a molecular mass of approximately 70 kDa. It plays an important role in angiogenesis facilitating blood vessel remodeling and maturation. The protein is mainly expressed in endothelial cells where it functions by binding to the Tie2 receptor partially disrupting the Angiopoietin-Tie2 signaling axis. This disruption modulates vascular stability and permeability. The lyophilized form of the ANG-2 protein is often used in research for functional assays like ANG-2 ELISA to quantify its levels in various biological samples.
Biological function summary

Human angiogenesis processes are significantly affected by Angiopoietin-2 which serves as an antagonist to Angiopoietin-1 in regulating vascular dynamics. Although it does not form a stable complex by itself ANG2 activity is closely linked to its interactions with the Tie2 receptor modulating signal pathways that govern endothelial cell survival and permeability. Because of its dual role either stabilizing or destabilizing blood vessels under different conditions ANG2 serves as an important regulator during both normal physiological events and pathophysiological conditions.

Pathways

Angiopoietin-2 significantly contributes to the angiogenesis signaling network. It is deeply involved in the VEGF (Vascular Endothelial Growth Factor) pathway where ANG2 acts to balance VEGF activities in angiogenesis and vessel permeability. The interplay between Angiopoietin-2 and other proteins such as Angiopoietin-1 which stabilizes the vessels is vital for the precise regulation of blood vessel homeostasis during normal and disturbed conditions.

Angiopoietin-2 has a prominent role in the pathology of diseases like cancer and diabetic retinopathy. In cancer excessive ANG2 expression may lead to increased tumor angiogenesis and metastasis making it a target for cancer therapies. In diabetic retinopathy elevated levels of ANG2 correlate with vascular leakage contributing to disease progression. The relationship between Angiopoietin-1 and Angiopoietin-2 in these conditions emphasizes their collective impact on vascular anomalies associated with these disorders.
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Product protocols

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Target data

Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling (PubMed : 15284220, PubMed : 19116766, PubMed : 19223473, PubMed : 9204896). Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1 (PubMed : 15284220, PubMed : 19116766, PubMed : 19223473, PubMed : 9204896). In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal (PubMed : 15284220, PubMed : 19116766, PubMed : 19223473, PubMed : 9204896). Involved in the regulation of lymphangiogenesis (PubMed : 32908006).
See full target information ANGPT2
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

BMC pediatrics 24:68 PubMed38245695

2024

The association between prolonged capillary refill time and microcirculation changes in children with sepsis.

Applications

Unspecified application

Species

Unspecified reactive species

Jaime Fernández-Sarmiento,Shirley Lamprea,Sofia Barrera,Lorena Acevedo,Catalina Duque,Manuela Trujillo,Valeria Aguirre,Carolina Jimenez

Microcirculation (New York, N.Y. : 1994) 30:e12829 PubMed37639384

2023

The association between hypoalbuminemia and microcirculation, endothelium, and glycocalyx disorders in children with sepsis.

Applications

Unspecified application

Species

Unspecified reactive species

Jaime Fernández-Sarmiento,Ricardo Hernández-Sarmiento,María Paula Salazar,Sofia Barrera,Valeria Castilla,Catalina Duque

Experimental and therapeutic medicine 14:3993-4000 PubMed29104621

2017

Abnormal expression of microRNA-575 leads to missed abortion through regulating apoptosis and angiogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Shuqin Xia,Yihui Zhen,Hongsheng Ma,Aiming Wang

Medicine 95:e4493 PubMed27512865

2016

Plasma angiopoietin-2 is persistently elevated after non-small cell lung cancer surgery and stimulates angiogenesis in vitro.

Applications

Unspecified application

Species

Human

Leng Zhou,Haidan Lan,Qinghua Zhou,Jianming Yue,Bin Liu

Annals of the rheumatic diseases 72:1265-7 PubMed23482472

2013

Antitumour necrosis factor-α therapy modulates angiopoietin-2 serum levels in non-diabetic ankylosing spondylitis patients.

Applications

Unspecified application

Species

Unspecified reactive species

Fernanda Genre,José A Miranda-Filloy,Raquel López-Mejias,Beatriz Carnero-López,Rodrigo Ochoa,Javier Rueda,Carlos González-Juanatey,Ricardo Blanco,Javier Llorca,Miguel A González-Gay
View all publications
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