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AB193693

Human ANGPT4 ELISA Kit

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Human ANGPT4 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human ANGPT4 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

ANG3, ANG4, ANGPT4, Angiopoietin-4, ANG-4, Angiopoietin-3, ANG-3

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Sandwich ELISA - Human ANGPT4 ELISA Kit (AB193693)
  • sELISA

Supplier Data

Sandwich ELISA - Human ANGPT4 ELISA Kit (AB193693)

Standard Curve in Assay Diluent B using ab193693 ANGPT4 Human ELISA Kit.

Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

Sandwich ELISA - Human ANGPT4 ELISA Kit (AB193693)
  • sELISA

Supplier Data

Sandwich ELISA - Human ANGPT4 ELISA Kit (AB193693)

Standard Curve in Assay Diluent A using ab193693 ANGPT4 Human ELISA Kit.

Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.25 ng/mL

Range

0.25 - 200 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's ANGPT4 Human ELISA Kit (ab193693) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human ANGPT4 in serum plasma and cell culture supernatants.

This assay employs an antibody specific for human ANGPT4 coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures ANGPT4 present in the samples. The wells are washed and biotinylated anti-human ANGPT4 antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of ANGPT4 bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" } ]
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Recovery

[ { "sample": "Serum", "range": "88 - 120 %", "average": "= 101.4" }, { "sample": "Plasma", "range": "67 - 91 %", "average": "= 83.13" }, { "sample": "Cell culture media", "range": "95 - 130 %", "average": "= 113.7" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab193693-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB193693-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB193693-CMP08", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB193693-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Angiopoietin-4 Standards (lyophilized)", "number":"AB193693-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB193693-CMP09", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB193693-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Pre-coated Human Angiopoietin-4 Microplate (12 strips x 8 wells)", "number":"AB193693-CMP04", "productcode":"" }, { "size":"1 x 200 µL", "name":"600X HRP-Streptavidin Concentrate", "number":"AB193693-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human Angiopoietin-4 detection antibody", "number":"AB193693-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Angiopoietin 4 also known as ANGPT4 or AGP4 is a secreted glycoprotein involved in vascular development. This protein has a molecular mass of around 56 kDa. ANGPT4 is expressed in a variety of tissues including the lung heart and kidney. It functions by interacting with the Tie receptors influencing vascular remodeling and angiogenesis. Angiopoietin 4 shares homology with other angiopoietins but has distinct regulatory roles compared to its counterparts.
Biological function summary

Angiopoietin 4 regulates angiogenesis and vascular permeability. It acts independently and does not part of a known protein complex. ANGPT4 contributes to the restructuring of blood vessels by promoting endothelial cell survival and migration. By balancing the actions of other angiopoietins such as Angiopoietin 1 it supports the maturation and stabilization of vascular networks impacting overall vascular function and growth.

Pathways

Angiopoietin 4 affects early morphogenetic signaling in vascular development. ANGPT4 operates within the angiopoietin-Tie signaling pathway important for angiogenesis and lymphangiogenesis. It interacts with Tie2 a receptor tyrosine kinase to mediate these effects. Within the pathway ANGPT4 coordinates with Angiopoietin 1 and Angiopoietin 2 modulating the balance between vessel stability and growth as required.

Angiopoietin 4 shows relevance to conditions like cancer and diabetic retinopathy. Abnormal expression or signaling of ANGPT4 can lead to defective vascular structures contributing to the pathology of these diseases. In cancer altered ANGPT4 and its interaction with Tie2 may disrupt normal angiogenesis facilitating tumor growth. Additionally in diabetic retinopathy ANGPT4 expression changes can exacerbate abnormal vessel formation highlighting its importance alongside Angiopoietin 2 in disease progression.
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Product protocols

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Target data

Binds to TEK/TIE2, modulating ANGPT1 signaling. Can induce tyrosine phosphorylation of TEK/TIE2. Promotes endothelial cell survival, migration and angiogenesis.
See full target information ANGPT4
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websiteProtocolBooklet
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