Human ANGPTL3 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human ANGPTL3 with a sensitivity of 103.25 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
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- Frontiers in pediatrics 11:11134842023Angiopoietin-like protein 3: a novel potential biomarker for nephrotic syndrome in children.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFujie Wen,Junchao Liu,Rufeng Dai,Sha Hong,Baowei Ji,Jiaojiao Liu,Jun Zhang,Xinli Han,Qianying Lv,Jialu Liu,Qian Shen,Hong XuPubMed 37266537
- Frontiers in cardiovascular medicine 9:9133632022ANGPTL3 Is Involved in the Post-prandial Response in Triglyceride-Rich Lipoproteins and HDL Components in Patients With Coronary Artery Disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXin Guo,Zhijie Huang,Jin Chen,Jiarui Hu,Die Hu,Daoquan Peng,Bilian YuPubMed 35845073
- World journal of clinical cases 10:2095-21052022Correlation between betatrophin/angiogenin-likeprotein3/lipoprotein lipase pathway and severity of coronary artery disease in Kazakh patients with coronary heart disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLian Qin,Rena Rehemuding,Aikeliyaer Ainiwaer,Xiang MaPubMed 35321188
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture media, Heparin Plasma, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 218.75 - 14000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 103.25 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Acts in part as a hepatokine that is involved in regulation of lipid and glucose metabolism (PubMed:11788823, PubMed:12909640, PubMed:23661675, PubMed:25495645). Proposed to play a role in the trafficking of energy substrates to either storage or oxidative tissues in response to food intake (By similarity). Has a stimulatory effect on plasma triglycerides (TG), which is achieved by suppressing plasma TG clearance via inhibition of LPL activity. The inhibition of LPL activity appears to be an indirect mechanism involving recruitment of proprotein convertases PCSK6 and FURIN to LPL leading to cleavage and dissociation of LPL from the cell surface; the function does not require ANGPTL3 proteolytic cleavage but seems to be mediated by the N-terminal domain, and is not inhibited by GPIHBP1 (PubMed:12097324, PubMed:19318355, PubMed:20581395). Can inhibit endothelial lipase, causing increased plasma levels of high density lipoprotein (HDL) cholesterol and phospholipids (PubMed:17110602, PubMed:19028676). Can bind to adipocytes to activate lipolysis, releasing free fatty acids and glycerol (PubMed:12565906). Suppresses LPL specifically in oxidative tissues which is required to route very low density lipoprotein (VLDL)-TG to white adipose tissue (WAT) for storage in response to food; the function may involve cooperation with circulating, liver-derived ANGPTL8 and ANGPTL4 expression in WAT (By similarity). Contributes to lower plasma levels of low density lipoprotein (LDL)-cholesterol by a mechanism that is independent of the canonical pathway implicating APOE and LDLR. May stimulate hypothalamic LPL activity (By similarity). ANGPTL3(17-221). In vitro inhibits LPL activity; not effective on GPIHBP1-stabilized LPL. Involved in angiogenesis. Binds to endothelial cells via integrin alpha-V/beta-3 (ITGAV:ITGB3), activates FAK, MAPK and Akt signaling pathways and induces cell adhesion and cell migration (PubMed:11877390). Secreted from podocytes, may modulate properties of glomerular endothelial cells involving integrin alpha-V/beta-3 and Akt signaling (PubMed:18535744). May increase the motility of podocytes. May induce actin filament rearrangements in podocytes implicating integrin alpha-V/beta-3 and Rac1 activation. Binds to hematopoietic stem cells (HSC) and is involved in the regulation of HSC activity probably implicating down-regulation of IKZF1/IKAROS (By similarity).
Alternative names
ANGPT5, UNQ153/PRO179, ANGPTL3, Angiopoietin-related protein 3, Angiopoietin-5, Angiopoietin-like protein 3, ANG-5
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Human ANGPTL3 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human ANGPTL3 with a sensitivity of 103.25 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture media, Heparin Plasma, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 218.75 - 14000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 103.25 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 8 | mean - | SD - | C.V. 6 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. 7.4 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 104 | Range 100 - 107 % |
Sample type EDTA Plasma | Average % = 96 | Range 83 - 117 % |
Sample type Cell culture media | Average % = 101 | Range 89 - 114 % |
Sample type Heparin Plasma | Average % = 95 | Range 86 - 103 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human ANGPTL3 ELISA Kit (ab254510) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of ANGPTL3 protein in cell culture media, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human ANGPTL3 with 103.25 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
ANGPTL3 (Angiopoietin-like 3) is a glycoprotein made in the liver and involved in lipid metabolism. ANGPTL3 can inhibit endothelial lipase and ANGPTL3 levels correlate with plasma HDL cholesterol, implicating it in modulation of HDL levels. ANGPTL3 expression is also associated with triglyceride levels, as loss of function mutations in ANGPTL3 is often found in individuals with low plasma triglyceride levels. Additionally, interference of ANGPTL3 interactions with LPL (the enzyme that hydrolyzes circulating triglycerides) in mice resulted in a reduction of serum triglyceride levels.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ANGPTL3 also known as angiopoietin-like protein 3 or betatrophin is a protein that functions mechanistically in lipid metabolism. It consists of approximately 70 kDa mass and is expressed mainly in the liver. ANGPTL3 is synthesized and secreted as a glycoprotein and it undergoes post-translational modifications important for its function. The protein exists predominantly in a monomeric form in circulation.
- Biological function summary
ANGPTL3 inhibits lipoprotein lipase (LPL) activity to regulate plasma lipid levels. It independently interacts with lipids and facilitates the storage of triglycerides making it an important modulator of lipid metabolism. ANGPTL3 does not form a stable complex with other proteins but modulates the presence and stability of LPL on the endothelial surface reducing lipid clearance. ANGPT1 ELISA can measure ANGPTL3 levels as part of routine assays including ANGPTL3 ELISA and beta-trophin ELISA.
- Pathways
ANGPTL3 plays a central role in the lipid metabolism and energy homeostasis pathways. In the lipid metabolism pathway ANGPTL3's ability to inhibit LPL links it to other proteins involved in lipid transport and storage such as LDL and HDL particles. Another related protein ANGPTL4 shares similar functions with ANGPTL3 indicating a level of redundancy and regulation within these pathways. ANGPTL3 like ANGPTL4 is a significant player in balancing lipid processing and storage.
- Associated diseases and disorders
ANGPTL3 strongly associates with hyperlipidemia and atherosclerosis. Abnormalities in ANGPTL3 levels can lead to altered lipid profiles raising the risk for cardiovascular diseases. Familial combined hypolipidemia involves mutations in the ANGPTL3 gene leading to drastically lower lipid levels. Disorders such as hypercholesterolemia also demonstrate connections with ANGPTL3 highlighting its role in lipid imbalance. These associations make the anti-ANGPTL3 antibodies important for the study and treatment of such lipid disorders.
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3 product images
Sandwich ELISA - Human ANGPTL3 ELISA Kit (ab254510)
Example of human ANGPTL3 standard curve in Sample Diluent NS.
The ANGPTL3 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human ANGPTL3 ELISA Kit (ab254510)
Interpolated concentrations of native ANGPTL3 in human serum and plasma samples.
The concentrations of ANGPTL3 were measured in duplicates, interpolated from the ANGPTL3 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 6.25%, plasma (heparin) 25%, and plasma (EDTA) 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ANGPTL3 concentration was determined to be 96.46 ng/mL in serum, 47.71 ng/mL in plasma (heparin), and 60.52 ng/mL in plasma (EDTA).
Sandwich ELISA - Human ANGPTL3 ELISA Kit (ab254510)
Serum from ten individual healthy human male donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ANGPTL3 concentration was determined to be 83.11 ng/mL with a range of 39.13 – 196.03 ng/mL.
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