Human Annexin A1 ELISA Kit is a sandwich ELISA designed to quantify Human Annexin A1 with a sensitivity of 0.13 ng/mL.
- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 0.313 - 20 ng/mL
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- Nature communications 14:38702023Single cell transcriptomics identifies distinct profiles in pediatric acute respiratory distress syndrome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTim Flerlage,Jeremy Chase Crawford,E Kaitlynn Allen,Danielle Severns,Shaoyuan Tan,Sherri Surman,Granger Ridout,Tanya Novak,Adrienne Randolph,Alina N West,Paul G ThomasPubMed 37391405
- Molecular cancer research : MCR 21:614-6272023JMJD6 Shapes a Pro-tumor Microenvironment via ANXA1-Dependent Macrophage Polarization in Breast Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBianca Cioni,Silvia Ratti,Annamaria Piva,Irene Tripodi,Matteo Milani,Francesca Menichetti,Tiziana Langella,Laura Botti,Loris De Cecco,Claudia Chiodoni,Daniele Lecis,Mario P ColomboPubMed 36867680
- Frontiers in medicine 9:7698132022Renal Expression of Annexin A1 Is Associated With the Severity of Renal Injury in Antineutrophil Cytoplasmic Autoantibody-Associated Vasculitis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRui-Xue Wang,Liang Wu,Su-Fang Chen,Zhi-Ying Li,Ming-Hui Zhao,Min ChenPubMed 35783659
- Experimental gerontology 158:1116512021Effects of resistance exercise and whey protein supplementation on skeletal muscle strength, mass, physical function, and hormonal and inflammatory biomarkers in healthy active older men: a randomised, double-blind, placebo-controlled trial.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCorbin Griffen,Michael Duncan,John Hattersley,Martin O Weickert,Alexander Dallaway,Derek RenshawPubMed 34896568
- Journal of neuroinflammation 18:1192021Annexin A1 protects against cerebral ischemia-reperfusion injury by modulating microglia/macrophage polarization via FPR2/ALX-dependent AMPK-mTOR pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXin Xu,Weiwei Gao,Lei Li,Jiheng Hao,Bin Yang,Tao Wang,Long Li,Xuesong Bai,Fanjian Li,Honglei Ren,Meng Zhang,Liyong Zhang,Jiyue Wang,Dong Wang,Jianning Zhang,Liqun JiaoPubMed 34022892
- BMC infectious diseases 21:2192021Low Annexin A1 level in HTLV-1 infected patients is a potential biomarker for the clinical progression and diagnosis of HAM/TSP.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBárbara Brasil Santana,Maria Alice Freitas Queiroz,Rodrigo Arcoverde Cerveira,Claudia Mendonça Rodrigues,Ednelza da Silva Graça Amoras,Carlos Araújo da Costa,Maisa Silva de Sousa,Ricardo Ishak,Luiz Ricardo Goulart,Antonio Carlos Rosário VallinotoPubMed 33632146
- Oncology letters 21:1412021Annexin A1 is a potential biomarker of bone metastasis in small cell lung cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPeng Chen,Jie Min,Hong Wu,Helong Zhang,Chaoli Wang,Guangguo Tan,Feng ZhangPubMed 33552260
- Scientific reports 10:172492020Application of small molecule FPR1 antagonists in the treatment of cancers.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDjevdet S Ahmet et. al.PubMed 33057069
- Journal for immunotherapy of cancer 8:2020Targeting ANXA1 abrogates Treg-mediated immune suppression in triple-negative breast cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFang Bai,Peng Zhang,Yipeng Fu,Hongliang Chen,Mingdi Zhang,Qianru Huang,Dan Li,Bin Li,Kejin WuPubMed 32300050
- Oncology letters 18:321-3292019Annexin A1-mediated inhibition of inflammatory cytokines may facilitate the resolution of inflammation in acute radiation-induced lung injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGaohua Han,Kaijin Lu,Wansong Xu,Sihui Zhang,Junxing Huang,Chunlei Dai,Guangzhi Sun,Jun YePubMed 31289503
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Tissue, Cell culture supernatant, Serum, Cell Lysate
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.313 - 20 ng/mL
- Assay time
- 4h
- Sensitivity
- = 0.13 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Plays important roles in the innate immune response as effector of glucocorticoid-mediated responses and regulator of the inflammatory process. Has anti-inflammatory activity (PubMed:8425544). Plays a role in glucocorticoid-mediated down-regulation of the early phase of the inflammatory response (By similarity). Contributes to the adaptive immune response by enhancing signaling cascades that are triggered by T-cell activation, regulates differentiation and proliferation of activated T-cells (PubMed:17008549). Promotes the differentiation of T-cells into Th1 cells and negatively regulates differentiation into Th2 cells (PubMed:17008549). Has no effect on unstimulated T cells (PubMed:17008549). Negatively regulates hormone exocytosis via activation of the formyl peptide receptors and reorganization of the actin cytoskeleton (PubMed:19625660). Has high affinity for Ca(2+) and can bind up to eight Ca(2+) ions (By similarity). Displays Ca(2+)-dependent binding to phospholipid membranes (PubMed:2532504, PubMed:8557678). Plays a role in the formation of phagocytic cups and phagosomes. Plays a role in phagocytosis by mediating the Ca(2+)-dependent interaction between phagosomes and the actin cytoskeleton (By similarity). Annexin Ac2-26. Functions at least in part by activating the formyl peptide receptors and downstream signaling cascades (PubMed:15187149, PubMed:22879591, PubMed:25664854). Promotes chemotaxis of granulocytes and monocytes via activation of the formyl peptide receptors (PubMed:15187149). Promotes rearrangement of the actin cytoskeleton, cell polarization and cell migration (PubMed:15187149). Promotes resolution of inflammation and wound healing (PubMed:25664854). Acts via neutrophil N-formyl peptide receptors to enhance the release of CXCL2 (PubMed:22879591).
Alternative names
ANX1, LPC1, ANXA1, Annexin A1, Annexin I, Annexin-1, Calpactin II, Calpactin-2, Chromobindin-9, Lipocortin I, Phospholipase A2 inhibitory protein, p35
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Human Annexin A1 ELISA Kit is a sandwich ELISA designed to quantify Human Annexin A1 with a sensitivity of 0.13 ng/mL.
- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 0.313 - 20 ng/mL
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Tissue, Cell culture supernatant, Serum, Cell Lysate
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.313 - 20 ng/mL
- Assay time
- 4h
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 0.13 ng/mL
- General recovery
- 99%
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Average CV | n 0 | C.V. 3.8 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Average CV | n 0 | C.V. 9.7 |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- Multi
- Storage information
- Please refer to protocols
Notes
ab222868 Human Annexin A1 ELISA Kit is designed for the quantitative measurement of Annexin A1 in plasma, serum, cell lysate and cell culture samples.
The kit employs a quantitative sandwich enzyme immunoassay technique that measures human Annexin A1 in approximately 4 hours. A polyclonal antibody specific for human Annexin A1 has been pre-coated onto a 96-well microplate with removable strips. Annexin A1 in standards and samples is sandwiched by the immobilized antibody and biotinylated polyclonal antibody specific for Annexin A1, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
Annexin A1, also known as lipocortin I, annexin I, chromobindin-9, and calpactin II, belongs to the annexin family of calcium-dependent phospholipid-binding proteins. The annexin proteins are structurally characterized by a core domain containing a conserved 70 amino acid sequence repeated four or eight times. This core is preceded by a unique N-terminal domain that varies in sequence and length and is thought to be responsible for specifying different annexin functions. Annexin A1 has an apparent relative molecular mass of 40 kDa with 346 amino acids and is preferentially located on the cytosolic face of the plasma membrane. It mimics the effect of steroids, mediates anti-inflammatory activity and is a potent inhibitor of phospholipase A2 activity. Annexin A1 regulates neutrophil extravasation by interacting with the formyl peptide receptor and triggers different signaling pathways.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Annexin A1 also known as ANXA1 or annexin 1 is a multifunctional protein with a molecular mass of approximately 37 kDa. This protein belongs to the annexin family and is distributed widely in various tissue types including the central nervous system respiratory system and immune cells. Annexin A1 functions by binding to phospholipids in a calcium-dependent manner influencing membrane-related processes and signal transduction. Its expression can be examined using techniques like annexin A1 immunohistochemistry and annexin A1 ELISA to study protein localization and concentration.
- Biological function summary
Annexin A1 is involved in modulating inflammatory responses and cell proliferation. It acts as a mediator in the resolution of inflammation by promoting apoptotic cell clearance and regulating leukocyte migration. Annexin A1 participates in complex interactions often associating with cell membrane phospholipids to exert its effects. It contributes significantly to cell adhesion migration and intracellular signaling events which are important for maintaining homeostasis and proper immune function.
- Pathways
Annexin A1 plays a significant role in the glucocorticoid signaling pathway and the formation of the cytoskeleton. It interacts with proteins like lipocortin 1 and phospholipase A2 impacting processes such as arachidonic acid metabolism and cytoskeletal reorganization. These interactions underline annexin A1's involvement in regulating inflammation and maintaining cellular architecture linking it to cellular processes that govern stress responses and cell movement.
- Associated diseases and disorders
Annexin A1 exhibits relevance to inflammatory diseases and cancer. It has been implicated in conditions such as asthma where it modulates inflammatory cell recruitment and activation. In cancer especially in breast cancer annexin A1 influences tumor progression by interacting with proteins like COX-2 affecting tumor cell invasion and metastasis. The protein's role in these conditions highlights its potential as both a biomarker and therapeutic target helping in understanding disease mechanisms and developing treatment strategies.
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Sandwich ELISA - Human Annexin A1 ELISA Kit (ab222868)
Example of human Annexin A1 standard curve.
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