Human Annexin A1 ELISA Kit

Product details

Human Annexin A1 ELISA Kit ab222868 is a sandwich ELISA to measure Human Annexin A1 in serum, plasma, cell culture supernatant, cell lysate, tissue with a sensitivity of 0.13 ng/ml.

How the assay works

The kit employs a quantitative sandwich enzyme immunoassay technique that measures human Annexin A1 in approximately 4 hours. A polyclonal antibody specific for human Annexin A1 has been pre-coated onto a 96-well microplate with removable strips. Annexin A1 in standards and samples is sandwiched by the immobilized antibody and biotinylated polyclonal antibody specific for Annexin A1, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

Assay Specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details

Human Annexin A1 ELISA Kit ab222868 protocol summary

1. Add standard or sample to appropriate wells. Incubate at room temperature
2. Wash and add prepared biotin antibody to each well. Incubate at room temperature.
3. Wash and add prepared Streptavidin-Peroxidase Conjugate. Incubate at room temperature
4. Add Chromogen Substrate to each well. Incubate at room temperature
5. Add Stop Solution to each well. Read at 450 nm immediately.

Annexin A1, also known as lipocortin I, annexin I, chromobindin-9, and calpactin II, belongs to the annexin family of calcium-dependent phospholipid-binding proteins. The annexin proteins are structurally characterized by a core domain containing a conserved 70 amino acid sequence repeated four or eight times. This core is preceded by a unique N-terminal domain that varies in sequence and length and is thought to be responsible for specifying different annexin functions. Annexin A1 has an apparent relative molecular mass of 40 kDa with 346 amino acids and is preferentially located on the cytosolic face of the plasma membrane. It mimics the effect of steroids, mediates anti-inflammatory activity and is a potent inhibitor of phospholipase A2 activity. Annexin A1 regulates neutrophil extravasation by interacting with the formyl peptide receptor and triggers different signaling pathways.