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AB222869

Human Annexin A10 ELISA Kit

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Human Annexin A10 ELISA Kit is a Sandwich ELISA for the measurement of Human Annexin A10 in Human in Cell/Tissue Extracts samples.

View Alternative Names

ANX14, ANXA10, Annexin A10, Annexin-10, Annexin-14

1 Images
Sandwich ELISA - Human Annexin A10 ELISA Kit (AB222869)
  • sELISA

Supplier Data

Sandwich ELISA - Human Annexin A10 ELISA Kit (AB222869)

Example of human Annexin A10 standard curve.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate, Tissue

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 89 pg/mL

Range

0.156 - 10 ng/mL

Assay time

4h

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

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Secondary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

ab222869 Human Annexin A10 ELISA Kit is designed for the quantitative measurement of Annexin A10 in plasma, serum, urine and cell culture samples.

The kit employs a quantitative sandwich enzyme immunoassay technique that measures human Annexin A10 in 4 hours. A polyclonal antibody specific for human Annexin A10 has been pre-coated onto a 96-well microplate with removable strips. Annexin A10 in standards and samples is sandwiched by the immobilized antibody and biotinylated polyclonal antibody specific for Annexin A10, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.

The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

Annexin A10, also known as ANXA10, Annexin-10, or Annexin-14, is a member of the annexin family of calcium-dependent phospholipid-binding proteins. Annexins have unique N-terminal domains and homologous C-terminal domains containing the calcium-dependent phospholipid-binding sites. Annexin A10 contains deduced 324 amino acids and weighs 37 kDa. It has several distinct features, including rare expression, a codon deletion in conserved repeat 3, and an unusual ablation of the type II calcium-binding sites in tetrad core repeats, indicating a calcium- and membrane-binding-independent function. Annexin A10 has been implicated in cellular functions in endocytosis and exocytosis, anticoagulant activity, cellular differentiation and proliferation, invasion, inhibition of calcium channels, and signal transduction pathways.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9.8" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.4" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Annexin A10 (ANXA10) also known as Annexin-10 is a protein with a molecular weight of approximately 37 kDa. You can find its expression mainly in the cytoplasm of epithelial cells especially in the gastrointestinal tract and liver. ANXA10 belongs to the annexin family which is known for calcium-dependent phospholipid binding. Its unique N-terminal region differentiates it from other annexin family members and may confer specific functions in cellular processes.
Biological function summary

ANXA10 functions as a calcium-dependent phospholipid-binding protein that regulates cell proliferation and differentiation. Though not part of a larger protein complex it may interact with cytoplasmic or membrane-associated proteins to perform its functions. ANXA10 helps maintain cellular integrity and possibly plays a role in the development of certain tissue types.

Pathways

ANXA10 plays a role in the cellular mechanisms involving vesicle trafficking and signal transduction. This protein contributes to the phospholipid metabolism and Annexin mechanisms working with other annexin proteins to mediate intracellular events. ANXA10 shares pathway relations with proteins such as Annexin A1 and Annexin A2 which also influence cell growth and signaling pathways.

Researchers have associated ANXA10 with liver cancer and gastric cancer. Altered expression of ANXA10 could contribute to tumor development and progression in these cancers. Studies show potential links with proteins like Annexin A8 in gastric cancer which makes ANXA10 a critical target of interest in cancer diagnostics and therapeutic research.
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Product protocols

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Target data

See full target information ANXA10
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websiteProtocolBooklet
en
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Product promise

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