AB264612

Human Annexin A2 ELISA Kit

Recombinant
SimpleStep
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Human Annexin A2 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Annexin A2 with a sensitivity of 1.86 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

ANX2, ANX2L4, CAL1H, LPC2D, ANXA2, Annexin A2, Annexin II, Annexin-2, Calpactin I heavy chain, Calpactin-1 heavy chain, Chromobindin-8, Lipocortin II, Placental anticoagulant protein IV, Protein I, p36, PAP-IV

2 Images

Sandwich ELISA - Human Annexin A2 ELISA Kit (AB264612)

sELISA

Supplier Data

Sandwich ELISA - Human Annexin A2 ELISA Kit (AB264612)

Interpolated concentrations of native Annexin A2 in human A431 cell extract based on a 4.4 μg/mL extract load.

The concentrations of Annexin A2 were measured in duplicate and interpolated from the Annexin A2 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

sELISA

Supplier Data

Sandwich ELISA - Human Annexin A2 ELISA Kit (AB264612)

Example of human Annexin A2 standard curve in Sample Diluent 1X Cell Extract Buffer PTR.

The Annexin A2 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 1.86 ng/mL

Range

8.8 - 100 ng/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human Annexin A2 ELISA Kit (ab264612) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Annexin A2 protein in cell lysate. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Annexin A2 with 1.86 ng/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Annexin A2 is a calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. May be involved in heat-stress response. Inhibits PCSK9-enhanced LDLR degradation, probably reduces PCSK9 protein levels via a translational mechanism, but also competes with LDLR for binding with PCSK9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 4, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.1" }, { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.6" } ]

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Recovery

[ { "sample": "Cell Lysate", "range": "94 - 99 %", "average": "= 97" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab264612-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB264612-CMP06", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB264612-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB264612-CMP12", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB264612-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB264612-CMP10", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Annexin A2 Lyophilized Recombinant Protein", "number":"AB264612-CMP09", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Annexin A2 Capture Antibody", "number":"AB264612-CMP01", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB264612-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Annexin A2 Detector Antibody", "number":"AB264612-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB264612-CMP05", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB264612-CMP08", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Annexin-2 also known as ANXA2 is a multifunctional protein of approximately 36 kDa. It belongs to the annexin family and is known for its strong calcium-dependent interactions with phospholipid membranes. Commonly expressed in endothelial cells it also appears in various tissues including epithelial cells and certain types of cancer cells. ANXA2 forms a heterotetramer complex with S100A10 also known as p11 which modulates its availability and function. Other names for ANXA2 include its gene designation M298 and its role in complexes often goes by ANXA2R.

Biological function summary

Annexin-2 acts beyond mere cellular structural support. It plays a part in fibrinolysis by acting as a co-receptor for plasminogen and tissue plasminogen activator promoting plasmin generation. The protein is also implicated in membrane trafficking signal transduction and organization of microdomains on cellular surfaces. As part of a complex with S100A10 ANXA2 can bind to cytoskeletal elements facilitating vesicle trafficking and influencing cell shape and motility. This complex assembly hints at ANXA2's importance in maintaining cellular dynamism and interplay with external signals.

Pathways

ANXA2 is integral in the regulation of the plasminogen activation pathway important for fibrinolytic activity. It also participates in the signal transduction pathways influencing cellular responses to stress and growth factors. ANXA2 interacts with various proteins like Src kinase and is involved in processes like the EGFR signaling pathway. This protein often crosstalks with the cytoskeletal network enabling the coupling of membrane dynamics with intracellular signaling cascades.

Annexin-2's dysregulation associates with conditions such as cancer and thrombosis. In cancer overexpression of ANXA2 links with tumor progression invasion and metastasis acting synergistically with proteins like Src to promote oncogenic pathways. In thrombosis ANXA2’s role in fibrinolysis becomes impaired contributing to excessive clot formation. Studies also indicate interactions with plasminogen-related proteins as being central to understanding its pathological roles in these diseases.

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Product protocols

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Target data

Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. May be involved in heat-stress response. Inhibits PCSK9-enhanced LDLR degradation, probably reduces PCSK9 protein levels via a translational mechanism but also competes with LDLR for binding with PCSK9 (PubMed : 18799458, PubMed : 22848640, PubMed : 24808179).. (Microbial infection) Binds M.pneumoniae CARDS toxin, probably serves as one receptor for this pathogen. When ANXA2 is down-regulated by siRNA, less toxin binds to human cells and less vacuolization (a symptom of M.pneumoniae infection) is seen.

See full target information ANXA2

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Publications (1)

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Frontiers in oncology 14:1484319 PubMed39850816

2025

Mechanistic insights into HPV-positivity in non-smokers and HPV-negativity in smokers with head and neck cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Markus Hoffmann,Susanne Hille,Asita Fazel,Martin Laudien,Susanne Wiegand,Martin Müller,Oliver J Müller,Elgar Susanne Quabius

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human Annexin A2 ELISA Kit

Sandwich ELISA - Human Annexin A2 ELISA Kit (AB264612)

Sandwich ELISA - Human Annexin A2 ELISA Kit (AB264612)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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