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AB283976

Human Annexin A3/ANXA3 ELISA Kit

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Human Annexin A3/ANXA3 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human Annexin A3/ANXA3 in Human in Biofluids, Cell/Tissue Extracts samples.

View Alternative Names

ANX3, ANXA3, Annexin A3, 35-alpha calcimedin, Annexin III, Annexin-3, Lipocortin III, Placental anticoagulant protein III, PAP-III

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Sandwich ELISA - Human Annexin A3/ANXA3 ELISA Kit (AB283976)
  • sELISA

Supplier Data

Sandwich ELISA - Human Annexin A3/ANXA3 ELISA Kit (AB283976)

Example of Human Annexin A3/ANXA3 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Urine, Plasma, Tissue, Serum, Cell Lysate

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.68 ng/mL

Range

1.563 - 100 ng/mL

Assay time

4h

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

The Human Annexin A3 ELISA Kit (ab283976) is designed for detection of Annexin A3 in human plasma, serum, urine, cell culture, and cell lysate samples. This assay employs a quantitative sandwich enzyme immunoassay technique that measures human annexin A3 in approximately 4 hours.

A polyclonal antibody specific for human annexin A3 has been pre-coated onto a 96-well microplate with removable strips. Annexin A3 in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for human annexin A3, which is recognized by a streptavidin-peroxidase (SP) conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 60, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.5" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 60, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.8" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Annexin A3 also known as ANXA3 is a 36 kDa protein that belongs to the annexin family. This family is known for calcium-dependent phospholipid-binding activity. ANXA3 specifically binds to phosphatidylserine a type of phospholipid found in cell membranes. It is expressed in various tissues with notable presence in myeloid cells particularly in the bone marrow and granulocytes.
Biological function summary

ANXA3 plays roles in membrane-related events including vesicle trafficking and exocytosis. It is not part of a large complex but associates with other proteins depending on its cellular function. ANXA3 can also regulate inflammation by modulating macrophage activation and may influence signal transduction pathways that are linked to immune responses.

Pathways

ANXA3 functions in both inflammation and apoptosis. It is closely related to the apoptosis pathway by interacting with signaling molecules like caspases which are critical for the execution of cell death. Additionally through its action in the inflammation pathway ANXA3 is known to interact with proteins such as NF-κB an important regulator of immune and inflammatory responses.

Alterations in ANXA3 expression or function associate with various conditions most notably cancer and autoimmune diseases. For instance in certain cancers ANXA3 may contribute to tumor growth and metastasis. In autoimmune diseases its regulation of macrophage activity can misbalance immune responses leading to pathogenesis. Proteins such as ANXA1 and S100A11 also play roles in these pathways and may function along with ANXA3 in disease progression.
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Product protocols

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Target data

Inhibitor of phospholipase A2, also possesses anti-coagulant properties. Also cleaves the cyclic bond of inositol 1,2-cyclic phosphate to form inositol 1-phosphate.
See full target information ANXA3
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