AB272212

Human Annexin A4 ELISA Kit (ANXA4)

Recombinant
SimpleStep
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Human Annexin A4 ELISA Kit (ANXA4) is a single-wash 90-min Simplestep used to quantify Human Annexin A4 (ANXA4) with a sensitivity of 16 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

ANX4, ANXA4, Annexin A4, 35-beta calcimedin, Annexin IV, Annexin-4, Carbohydrate-binding protein p33/p41, Chromobindin-4, Endonexin I, Lipocortin IV, P32.5, PP4-X, Placental anticoagulant protein II, Protein II, PAP-II

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Sandwich ELISA - Human Annexin A4 ELISA Kit (ANXA4) (AB272212)

sELISA

Supplier Data

Sandwich ELISA - Human Annexin A4 ELISA Kit (ANXA4) (AB272212)

Example of human Annexin A4 standard curve in 1X Cell Extraction Buffer PTR

sELISA

Supplier Data

Sandwich ELISA - Human Annexin A4 ELISA Kit (ANXA4) (AB272212)

Interpolated concentrations of native Annexin A4 in human HepG2 sample based on a 1 μg/mL extract load and lung sample based on an 8 μg/mL extract load.

The concentrations of Annexin A4 were measured in duplicate and interpolated from the Annexin A4 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Annexin A4 concentration was determined to be 1,767 pg/mL in HepG2 extract and 1,094 pg/mL in lung extract.

Key facts

Detection method

Colorimetric

Sample types

Tissue Extracts, Cell Lysate

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 16 pg/mL

Range

39.06 - 2500 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human Annexin A4 ELISA kit (ab272212) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Annexin A4 protein in cell and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Annexin A4 with 16 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less

-High sensitivity, specificity and reproducibility from superior antibodies

-Fully validated in biological samples

-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Annexin A4 (ANXA4) is a calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. Human Annexin A4 amino acids sequence is 92% identical to mouse and rat Annexin A4 sequence.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 10, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.8" } ]

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Recovery

[ { "sample": "Tissue Extracts", "range": "85 - 98 %", "average": "= 92" }, { "sample": "Cell Lysate", "range": "101 - 111 %", "average": "= 106" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab272212-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB272212-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB272212-CMP12", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB272212-CMP13", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Annexin A4 (ANXA4) Lyophilized Recombinant Protein", "number":"AB272212-CMP09", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB272212-CMP11", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Annexin A4 (ANXA4) Capture Antibody", "number":"AB272212-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB272212-CMP10", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Annexin A4 (ANXA4) Detector Antibody", "number":"AB272212-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB272212-CMP05", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB272212-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB272212-CMP07", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Annexin-4 also known as ANXA4 is a protein with a molecular mass of approximately 36 kDa. It belongs to the annexin family characterized by their ability to bind phospholipids in a calcium-dependent manner. Annexin-4 is widely expressed in various tissues including epithelial cells in the gastrointestinal tract kidney and placenta. This protein associates with the inner surface of the plasma membrane and plays roles in processes like vesicle trafficking and membrane dynamics.

Biological function summary

The protein functions in cellular processes involving membrane-related activities. It does not appear to be part of a larger stable complex but researchers have observed its interaction with cell membranes and involvement in membrane repair and cell signal transduction. Annexin-4 can mediate interactions between the cytoskeleton and the plasma membrane impacting cell motility and structure.

Pathways

Annexin-4 functions in the phospholipid signaling pathways particularly influencing pathways related to calcium signaling. In the calcium-dependent signaling pathway Annexin-4 regulates cellular responses by cooperating with proteins such as calmodulin and other annexins contributing to processes like cell proliferation and apoptosis.

Annexin-4 has shown associations with cancer and inflammatory diseases. Abnormal expression of Annexin-4 has been observed in certain cancer types where it may affect tumor metastasis and progression by modifying cellular adhesion and migration. Additionally its interactions with inflammation-related proteins such as interleukins suggest its involvement in inflammatory responses and disorders like inflammatory bowel disease. Researchers continue to explore its potential as a biomarker for early detection and as a target for therapeutic intervention.

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Product protocols

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Target data

Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis.

See full target information ANXA4

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human Annexin A4 ELISA Kit (ANXA4)

Sandwich ELISA - Human Annexin A4 ELISA Kit (ANXA4) (AB272212)

Sandwich ELISA - Human Annexin A4 ELISA Kit (ANXA4) (AB272212)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Assay Kits

Assay Kits

Primary Antibodies

Cell Lines & Lysates

Product promise