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AB283993

Human Annexin-A7/ANXA7 ELISA Kit

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Human Annexin-A7/ANXA7 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human Annexin-A7/ANXA7 in Human in Cell/Tissue Extracts samples.

View Alternative Names

ANX7, SNX, OK/SW-cl.95, ANXA7, Annexin A7, Annexin VII, Annexin-7, Synexin

1 Images
Sandwich ELISA - Human Annexin-A7/ANXA7 ELISA Kit (AB283993)
  • sELISA

Supplier Data

Sandwich ELISA - Human Annexin-A7/ANXA7 ELISA Kit (AB283993)

Example of Human Annexin-A7/ANXA7 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.45 ng/mL

Range

0.625 - 40 ng/mL

Assay time

4h

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

The Human Annexin A7/ANXA7 ELISA Kit (ab283993) is designed for detection of annexin A7 in human cell lysate samples. This assay employs a quantitative sandwich enzyme immunoassay technique that measures human annexin A7 in approximately 4 hours.

A polyclonal antibody specific for human annexin A7 has been pre-coated onto a 96-well microplate with removable strips. Annexin A7 in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for human annexin A7, which is recognized by a streptavidin-peroxidase (SP) conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The colour development is stopped and the intensity of the colour is measured.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 60, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.1" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 60, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.2" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab283993-1x96Tests", "assetComponentDetails": [ { "size":"2 x 30 mL", "name":"20X Wash Buffer Concentrate", "number":"AB283993-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB283993-CMP09", "productcode":"" }, { "size":"1 x 8 mL", "name":"Chromogen Substrate", "number":"AB283993-CMP04", "productcode":"" }, { "size":"1 x 120 µL", "name":"50X Biotinylated Human Annexin-A7/ANXA7 Antibody", "number":"AB283993-CMP07", "productcode":"" }, { "size":"3 x 1 Unit", "name":"Sealing Tapes", "number":"AB283993-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human Annexin-A7/ANXA7 Microplate", "number":"AB283993-CMP05", "productcode":"" }, { "size":"1 x 30 mL", "name":"10X Diluent N Concentrate", "number":"AB283993-CMP02", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Annexin-A7/ANXA7 Standard", "number":"AB283993-CMP01", "productcode":"" }, { "size":"1 x 80 µL", "name":"100X Streptavidin-Peroxidase Conjugate", "number":"AB283993-CMP10", "productcode":"" }, { "size":"1 x 2 mL", "name":"1X Standard Diluent", "number":"AB283993-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Annexin-7 (ANXA7) also known as synexin is a calcium-dependent phospholipid-binding protein. It has a molecular weight of approximately 51 kDa. This protein is expressed in various tissues with notable expression in the heart and brain. Mechanically ANXA7 plays a role in membrane fusion and exocytosis which are critical processes for cellular communication and transport.
Biological function summary

Annexin-7 functions as an important player in cell proliferation and signal transduction. It forms part of the annexin family of proteins which are known for their role in calcium ion binding and membrane-related activities. Annexin-7 has a role in linking Ca2+ signaling with phospholipid membrane dynamics which suggests its involvement in cellular events like growth and differentiation.

Pathways

Annexin-7 influences both the MAPK and EGFR signaling pathways. These pathways are essential for regulating cell division apoptosis and cellular response to external stimuli. Within these pathways Annexin-7 interacts with proteins such as Ras and PI3K which are important for propagating signals that control cellular behaviors.

Abnormalities in Annexin-7 expression and function associate it with cancer and schizophrenia. In cancer altered ANXA7 function may disrupt normal cell communication and growth pathways contributing to tumorigenesis. In schizophrenia disrupted calcium signaling involving ANXA7 may affect neuronal communication and brain function. In these conditions Annexin-7 interacts with proteins like p53 and NMDA receptors which are critical to the pathology of cancer and neurological disorders respectively.
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Product protocols

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Target data

Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis.
See full target information ANXA7
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websiteProtocolBooklet
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