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AB223863

Human Annexin V ELISA Kit

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(1 Review)

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(2 Publications)

Human Annexin V ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Annexin V with a sensitivity of 23.4 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

ANX5, ENX2, PP4, ANXA5, Annexin A5, Anchorin CII, Annexin V, Annexin-5, Calphobindin I, Endonexin II, Lipocortin V, Placental anticoagulant protein 4, Placental anticoagulant protein I, Thromboplastin inhibitor, Vascular anticoagulant-alpha, CPB-I, PAP-I, VAC-alpha

5 Images
ELISA - Human Annexin V ELISA Kit (AB223863)
  • ELISA

Supplier Data

ELISA - Human Annexin V ELISA Kit (AB223863)
Sandwich ELISA - Human Annexin V ELISA Kit (AB223863)
  • sELISA

Unknown

Sandwich ELISA - Human Annexin V ELISA Kit (AB223863)

Interpolated concentrations of native Annexin V in human HepG2 cell extract based on a 1 μg/mL extract load and heart and brain tissue extract samples based on a 2 μg/mL extract load. The concentrations of Annexin V were measured in duplicate and interpolated from the Annexin V standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Annexin V concentration was determined to be 1299 pg/mL in HepG2 cell extract, 2159 pg/mL in human heart tissue extract, and 2585 pg/mL in human brain tissue extract.

Sandwich ELISA - Human Annexin V ELISA Kit (AB223863)
  • sELISA

Unknown

Sandwich ELISA - Human Annexin V ELISA Kit (AB223863)

Example of human Annexin V standard curve in 1X Cell Extraction Buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Annexin V ELISA Kit (AB223863)
  • sELISA

Unknown

Sandwich ELISA - Human Annexin V ELISA Kit (AB223863)

Interpolated concentrations of native Annexin V in human urine and HepG2 cell culture supernatant (5 days) samples.

The concentrations of Annexin V were measured in duplicates, interpolated from the Annexin V standard curves and corrected for sample dilution. Undiluted samples are as follows : urine 20% and HepG2 cell culture supernatant 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Annexin V concentration was determined to be 1601 pg/mL in neat urine and 14,220 pg/mL in neat HepG2 Cell Culture Supernatant.

Sandwich ELISA - Human Annexin V ELISA Kit (AB223863)
  • sELISA

Unknown

Sandwich ELISA - Human Annexin V ELISA Kit (AB223863)

Example of human Annexin V standard curve in Sample Diluent NS + Enhancer.

Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Urine, Cell culture extracts, Tissue Extracts, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 23.4 pg/mL

Range

46.9 - 3000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Annexin V ELISA Kit ab223863 is a rapid single-wash 90-min Sandwich ELISA to measure Human Annexin V in cell culture extracts, cell culture supernatant, tissue extracts, urine. This SimpleStep sensitivity is 23.4 pg/mL.

How the assay works

Human Annexin V SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human Annexin V ELISA Kit ab223863 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-Annexin V/ANXA5 antibody [EPR20167-5] - BSA and Azide free (Capture) ab242907
- Anti-Annexin V/ANXA5 antibody [EPR20167-52] - BSA and Azide free (Detector) ab242907

Annexin V is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade. Annexin V is a 36 kDa protein that binds to phospholipids in a calcium-dependent manner. Annexin V preferentially binds phosphatidylserine, in competition with prothrombin, leading to inhibition of blood coagulation at sites of injury preceding contact between lipid components and coagulation factors that initiate thrombosis. The ability of Annexin V to bind to phosphatidylserine makes it an attractive reagent in detecting apoptotic cells.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.8" }, { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.8" } ]
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Recovery

[ { "sample": "Cell culture extracts", "range": "86 - 96 %", "average": "= 90" }, { "sample": "Urine", "range": "96 - 101 %", "average": "= 98" }, { "sample": "Tissue Extracts", "range": "100 - 105 %", "average": "= 103" }, { "sample": "Cell culture media", "range": "99 - 114 %", "average": "= 107" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Annexin V also known as ANXA5 is a protein with a mass of approximately 35-36 kDa. This protein belongs to the annexin family which includes proteins capable of binding to phospholipids in a calcium-dependent manner. Annexin V is mostly expressed in placental tissue though it can be found in various other tissues like the vascular endothelium. Researchers often utilize annexin V for staining dead cells or identifying apoptosis using assays such as annexin V FITC or 7-AAD staining. It forms part of the annexin V binding buffer which enhances its binding affinity.
Biological function summary

Annexin V has an important role in cell membrane dynamics and signaling. The protein does not function as part of a larger complex but its ability to bind negatively charged phospholipids like phosphatidylserine makes it important in apoptosis. During apoptosis phosphatidylserine translocates to the outer leaflet of the plasma membrane where annexin V marks these apoptotic cells. This property enables annexin V-based assays to detect early stages of apoptosis.

Pathways

Annexin V involvement is seen in pathways associated with blood coagulation and apoptosis. In the apoptosis pathway annexin V interacts with apoptotic markers to identify dying cells. It relates to proteins such as caspases which are key executors of apoptosis. In coagulation annexin V has been shown to interact with prothrombinase complex and can affect calcium ion concentration thereby influencing blood clotting processes.

Annexin V plays a role in antiphospholipid syndrome and cardiovascular diseases. Annexin V has been researched for its connection to antiphospholipid antibodies where it affects normal annexin V function impacting clot formation. In cardiovascular diseases annexin V can interact with proteins such as thrombomodulin influencing thrombotic and atherosclerotic pathways. Understanding these interactions helps researchers develop potential therapeutic approaches for related conditions.
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Product protocols

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Target data

This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
See full target information ANXA5
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Pharmaceuticals (Basel, Switzerland) 17: PubMed39204206

2024

EZH2 Inhibition to Counteract Oral Cancer Progression through Wnt/β-Catenin Pathway Modulation.

Applications

Unspecified application

Species

Unspecified reactive species

Michela Campolo,Sarah Adriana Scuderi,Alessia Filippone,Valentina Bova,Sofia Paola Lombardo,Lorenzo Colarossi,Serena Sava,Anna Paola Capra,Federica De Gaetano,Marco Portelli,Angela Militi,Emanuela Esposito,Irene Paterniti

Nature methods 19:1449-1460 PubMed36280722

2022

Geometric engineering of organoid culture for enhanced organogenesis in a dish.

Applications

Unspecified application

Species

Unspecified reactive species

Sunghee Estelle Park,Shawn Kang,Jungwook Paek,Andrei Georgescu,Jeehan Chang,Alex Yoon Yi,Benjamin J Wilkins,Tatiana A Karakasheva,Kathryn E Hamilton,Dan Dongeun Huh
View all publications
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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