Human Annexin V ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Annexin V with a sensitivity of 23.4 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Images
Publications
Filter by
- Nature methods 19:1449-14602022Geometric engineering of organoid culture for enhanced organogenesis in a dish.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSunghee Estelle Park,Shawn Kang,Jungwook Paek,Andrei Georgescu,Jeehan Chang,Alex Yoon Yi,Benjamin J Wilkins,Tatiana A Karakasheva,Kathryn E Hamilton,Dan Dongeun HuhPubMed 36280722
Key facts
- Detection method
- Colorimetric
- Sample types
- Urine, Cell culture extracts, Tissue Extracts, Cell culture supernatant
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 46.9 - 3000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 23.4 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
The protein expressed by gene ANXA5 is an anticoagulant protein that indirectly inhibits the thromboplastin-specific complex, a component involved in the blood coagulation cascade. This supplementary information is collated from multiple sources and compiled automatically.
Alternative names
ANX5, ENX2, PP4, ANXA5, Annexin A5, Anchorin CII, Annexin V, Annexin-5, Calphobindin I, Endonexin II, Lipocortin V, Placental anticoagulant protein 4, Placental anticoagulant protein I, Thromboplastin inhibitor, Vascular anticoagulant-alpha, CPB-I, PAP-I, VAC-alpha
What's included?
Recommended products
Human Annexin V ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Annexin V with a sensitivity of 23.4 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Urine, Cell culture extracts, Tissue Extracts, Cell culture supernatant
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 46.9 - 3000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 23.4 pg/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Supernatant | n 5 | C.V. 3.8 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Supernatant | n 3 | C.V. 10.8 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture extracts | Average % = 90 | Range 86 - 96 % |
Sample type Urine | Average % = 98 | Range 96 - 101 % |
Sample type Tissue Extracts | Average % = 103 | Range 100 - 105 % |
Sample type Cell culture media | Average % = 107 | Range 99 - 114 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human Annexin V ELISA Kit ab223863 is a rapid single-wash 90-min Sandwich ELISA to measure Human Annexin V in cell culture extracts, cell culture supernatant, tissue extracts, urine. This SimpleStep sensitivity is 23.4 pg/mL.
How the assay works
Human Annexin V SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Human Annexin V ELISA Kit ab223863 protocol summary
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:
- Anti-Annexin V/ANXA5 antibody [EPR20167-5] - BSA and Azide free (Capture) Anti-Annexin V/ANXA5 antibody [EPR20167-52] - BSA and Azide free (Detector) ab242907
- Anti-Annexin V/ANXA5 antibody [EPR20167-52] - BSA and Azide free (Detector) Anti-Annexin V/ANXA5 antibody [EPR20167-52] - BSA and Azide free (Detector) ab242907
Annexin V is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade. Annexin V is a 36 kDa protein that binds to phospholipids in a calcium-dependent manner. Annexin V preferentially binds phosphatidylserine, in competition with prothrombin, leading to inhibition of blood coagulation at sites of injury preceding contact between lipid components and coagulation factors that initiate thrombosis. The ability of Annexin V to bind to phosphatidylserine makes it an attractive reagent in detecting apoptotic cells.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Annexin V also known as ANXA5 is a protein with a mass of approximately 35-36 kDa. This protein belongs to the annexin family which includes proteins capable of binding to phospholipids in a calcium-dependent manner. Annexin V is mostly expressed in placental tissue though it can be found in various other tissues like the vascular endothelium. Researchers often utilize annexin V for staining dead cells or identifying apoptosis using assays such as annexin V FITC or 7-AAD staining. It forms part of the annexin V binding buffer which enhances its binding affinity.
- Biological function summary
Annexin V has an important role in cell membrane dynamics and signaling. The protein does not function as part of a larger complex but its ability to bind negatively charged phospholipids like phosphatidylserine makes it important in apoptosis. During apoptosis phosphatidylserine translocates to the outer leaflet of the plasma membrane where annexin V marks these apoptotic cells. This property enables annexin V-based assays to detect early stages of apoptosis.
- Pathways
Annexin V involvement is seen in pathways associated with blood coagulation and apoptosis. In the apoptosis pathway annexin V interacts with apoptotic markers to identify dying cells. It relates to proteins such as caspases which are key executors of apoptosis. In coagulation annexin V has been shown to interact with prothrombinase complex and can affect calcium ion concentration thereby influencing blood clotting processes.
- Associated diseases and disorders
Annexin V plays a role in antiphospholipid syndrome and cardiovascular diseases. Annexin V has been researched for its connection to antiphospholipid antibodies where it affects normal annexin V function impacting clot formation. In cardiovascular diseases annexin V can interact with proteins such as thrombomodulin influencing thrombotic and atherosclerotic pathways. Understanding these interactions helps researchers develop potential therapeutic approaches for related conditions.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
ELISA - Human Annexin V ELISA Kit (ab223863)
Sandwich ELISA - Human Annexin V ELISA Kit (ab223863)
Example of human Annexin V standard curve in Sample Diluent NS + Enhancer.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Annexin V ELISA Kit (ab223863)
Interpolated concentrations of native Annexin V in human HepG2 cell extract based on a 1 μg/mL extract load and heart and brain tissue extract samples based on a 2 μg/mL extract load. The concentrations of Annexin V were measured in duplicate and interpolated from the Annexin V standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Annexin V concentration was determined to be 1299 pg/mL in HepG2 cell extract, 2159 pg/mL in human heart tissue extract, and 2585 pg/mL in human brain tissue extract.
Sandwich ELISA - Human Annexin V ELISA Kit (ab223863)
Example of human Annexin V standard curve in 1X Cell Extraction Buffer PTR.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Annexin V ELISA Kit (ab223863)
Interpolated concentrations of native Annexin V in human urine and HepG2 cell culture supernatant (5 days) samples.
The concentrations of Annexin V were measured in duplicates, interpolated from the Annexin V standard curves and corrected for sample dilution. Undiluted samples are as follows: urine 20% and HepG2 cell culture supernatant 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Annexin V concentration was determined to be 1601 pg/mL in neat urine and 14,220 pg/mL in neat HepG2 Cell Culture Supernatant.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com