Human Antithrombin III / ATIII ELISA Kit
- Recombinant
- SimpleStep
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(1 Publication)
Human Antithrombin III / ATIII ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Antithrombin III / ATIII with a sensitivity of 0.33 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
View Alternative Names
AT3, PRO0309, SERPINC1, Antithrombin-III, ATIII, Serpin C1
- ELISA
Supplier Data
ELISA - Human Antithrombin III / ATIII ELISA Kit (AB222507)
- sELISA
Supplier Data
Sandwich ELISA - Human Antithrombin III / ATIII ELISA Kit (AB222507)
Serum from ten individual healthy human female donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Antithrombin III concentration was determined to be 77.20 μg/mL with a range of 34.09 – 121.12 μg/mL.
- sELISA
Supplier Data
Sandwich ELISA - Human Antithrombin III / ATIII ELISA Kit (AB222507)
Interpolated concentrations of native Antithrombin III in human serum and plasma samples.
The concentrations of Antithrombin III were measured in duplicates, interpolated from the Antithrombin III standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 4,000 plasma (citrate) 1 : 8,000, plasma (EDTA) 1 : 4,000, and plasma (heparin) 1 : 4,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Antithrombin III concentration was determined to be 157.69 μg/mL in neat serum, 279.95 μg/mL in neat plasma (citrate), 160.27 μg/mL in neat plasma (EDTA), and 224.88 μg/mL in neat plasma (heparin).
- sELISA
Supplier Data
Sandwich ELISA - Human Antithrombin III / ATIII ELISA Kit (AB222507)
Example of human Antithrombin III standard curve.
Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Supplier Data
Sandwich ELISA - Human Antithrombin III / ATIII ELISA Kit (AB222507)
Representative image of Human Antithrombin III / ATIII ELISA Kit (ab222507)
Components shown from left to right :
- Sample diluent NS
- Denaturant
- 10X Human Antithrombin III / ATIII Capture Antibody
- 10X Human Antithrombin III / ATIII Detector Antibody
- Human Antithrombin III / ATIII Lyophilised Purified Protein (2 vials)
- 50X Cell Extraction Enhancer Solution (ab193971)
- Stop Solution
- TMB solution
- 10X Wash Buffer PT (ab206977)
- Antibody Diluent 5BC
SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.
Plate Seals - shown underneath bottles / vials.
Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.
Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit
- sELISA
Supplier Data
Sandwich ELISA - Human Antithrombin III / ATIII ELISA Kit (AB222507)
Interpolated concentrations of native Antithrombin III in human milk and human Hep G2 supernatant samples and spiked Antithrombin III in human urine.
The concentrations of Antithrombin III were measured in duplicates, interpolated from the Antithrombin III standard curves and corrected for sample dilution. Undiluted samples are as follows : milk 3.13%, Hep G2 supernatant 6.25%, and urine 6.25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Antithrombin III concentration was determined to be 810.16 ng/mL in neat milk, 218.86 ng/mL in neat Hep G2 supernatant, and 618.60 ng/mL in neat spiked urine.
Reactivity data
Product details
Human Antithrombin III / ATIII ELISA Kit ab222507 is a rapid single-wash 90-min Sandwich ELISA to measure Human Antithrombin III / ATIII in cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, serum, urine. This SimpleStep sensitivity is 0.33 ng/mL.
How the assay works
Human Antithrombin III / ATIII SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Human Antithrombin III / ATIII ELISA Kit ab222507 protocol summary
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:
- Anti-Antithrombin III/ATIII antibody [EP5372] - BSA and Azide free (Capture) ab244918
- Anti-Antithrombin III/ATIII antibody [EPR20132-63] - BSA and Azide free (Detector) ab244918
Antithrombin-III (ATIII), also known as Serpin C1, is a 58 kDa extracellular glycoprotein belonging to the Serpin serine protease inhibitor superfamily. Antithrombin III functions primarily as a blood anticoagulant but also modulates inflammatory cytokine expression. Human Antithrombin-III is synthesized in the liver and is secreted as a 432 amino acid (aa) mature protein. Most circulating Antithrombin-III (90-95%) is the fully glycosylated a-isoform while the partially glycosylated Я-isoform constitutes 5-10%. The biological activity of Antithrombin-III is inherently low and is enhanced up to 1000-fold in the presence of heparin or heparin-like glycosaminoglycans. The Я-isoform of Antithrombin-III has a higher affinity for heparin. Mature human Antithrombin-III shares 90% sequence homology with mature mouse Antithrombin-III. Human Antithrombin-III inactivates thrombin and other blood coagulation factors. Human adult Antithrombin-III levels in plasma are relatively constant. However, reduction of Antithrombin-III is observed in individuals with diabetes or sepsis. Neonates also exhibit low levels of Serpin C1 in plasma.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Precision
Recovery
What's included?
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Antithrombin III plays a critical role in blood clotting by specifically inhibiting clotting factors. It forms complexes with its target enzymes leading to their inactivation and preventing excessive thrombin production. The protein does not form part of larger complexes but acts independently to exert its anticoagulant function. The normal range for antithrombin 3 levels in plasma is important for ensuring a balanced blood coagulation system. Its function helps prevent inappropriate clot formation and ensures smooth blood flow through the circulatory system.
Pathways
Antithrombin III is integral to the coagulation cascade. It is primarily involved in the intrinsic and common coagulation pathways where it regulates thrombin and Factor Xa activity. This regulation prevents the unchecked formation of fibrin clots which can lead to thrombosis. ATIII works closely with proteins like heparin cofactor II and protein C which also modulate the coagulation pathway emphasizing its role in maintaining hemostatic balance. Its function is essential in controlling the coagulation cascade and ensuring that it proceeds appropriately without leading to pathology.
Product protocols
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Target data
Publications (1)
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Thrombosis journal 21:3 PubMed36624481
2023
Applications
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Species
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