AB108801

Human Antithrombin III ELISA Kit

4

(1 Review)

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(3 Publications)

Human Antithrombin III ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human Antithrombin III in Human in Biofluids samples.

View Alternative Names

AT3, PRO0309, SERPINC1, Antithrombin-III, ATIII, Serpin C1

7 Images

Sandwich ELISA - Human Antithrombin III ELISA Kit (AB108801)

sELISA

Supplier Data

Sandwich ELISA - Human Antithrombin III ELISA Kit (AB108801)

Representative standard curve using ab108801.

Sandwich ELISA - Human Antithrombin III ELISA Kit (AB108801)

sELISA

Supplier Data

Sandwich ELISA - Human Antithrombin III ELISA Kit (AB108801)

Representative standard curve using ab108801.

ELISA - Human Antithrombin III ELISA Kit (AB108801)

ELISA

Lab

ELISA - Human Antithrombin III ELISA Kit (AB108801)

ELISA : Antithrombin III Human ELISA Kit.

Antithrombin III in human biological fluids (duplicates +/- SD). The samples were differently diluted (serum and Li-heparine plasma : 1e4-1e5; CSF : 1e2-1e3; milk : 10-100; saliva : 5-100; urine : neat).

ELISA - Human Antithrombin III ELISA Kit (AB108801)

ELISA

Lab

ELISA - Human Antithrombin III ELISA Kit (AB108801)

ELISA : Antithrombin III Human ELISA Kit.

Linearity : Antithrombin III in samples at different diutions, corrected for the factor of dilution (duplicates +/- SD), showing ng x mL^-1 on y-axis.

ELISA - Human Antithrombin III ELISA Kit (AB108801)

ELISA

Lab

ELISA - Human Antithrombin III ELISA Kit (AB108801)

ELISA : Antithrombin III Human ELISA Kit.

Linearity : Antithrombin III in samples at different diutions, corrected for the factor of dilution (duplicates +/- SD).

ELISA - Human Antithrombin III ELISA Kit (AB108801)

ELISA

Lab

ELISA - Human Antithrombin III ELISA Kit (AB108801)

ELISA : Antithrombin III Human ELISA Kit.

Antithrombin III in cell culture supernatants (duplicates +/- SD), tested in the dilution range of 1 : 1-1 : 100.

ELISA - Human Antithrombin III ELISA Kit (AB108801)

ELISA

Lab

ELISA - Human Antithrombin III ELISA Kit (AB108801)

ELISA : Antithrombin III Human ELISA Kit.

Linearity : Antithrombin III in samples at different diutions, corrected for the factor of dilution (duplicates +/- SD).

Key facts

Detection method

Colorimetric

Sample types

Cerebral Spinal Fluid, Milk, Plasma, Saliva, Serum, Urine

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.99 ng/mL

Range

1.563 - 100 ng/mL

Assay time

4h

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Abcam's Antithrombin III Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Antithrombin III in urine, plasma, milk, saliva, cerebrospinal fluid, serum, and cell culture supernatants.

An antithrombin III specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an antithrombin III specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of antithrombin III captured in plate.

The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.6" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab108801-1x96Tests", "assetComponentDetails": [ { "size":"2 x 30 mL", "name":"20X Wash Buffer Concentrate", "number":"AB108801-CMP06", "productcode":"" }, { "size":"1 x 120 µL", "name":"50X Biotinylated Human Antithrombin III Antibody", "number":"AB108801-CMP01", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Solution", "number":"AB108801-CMP08", "productcode":"" }, { "size":"1 x 30 mL", "name":"10X Diluent N Concentrate", "number":"AB108801-CMP02", "productcode":"" }, { "size":"1 x 7 mL", "name":"Chromogen Substrate", "number":"AB108801-CMP03", "productcode":"" }, { "size":"1 x 80 µL", "name":"100X Streptavidin-Peroxidase Conjugate", "number":"AB108801-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Antithrombin III Microplate (12 x 8 well strips)", "number":"AB108801-CMP04", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Antithrombin III Standard", "number":"AB108801-CMP05", "productcode":"" }, { "size":"3 x 1 Unit", "name":"Sealing Tapes", "number":"AB108801-CMP07", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

Multi

Storage information

Please refer to protocols

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Antithrombin III also known as ATIII or antithrombin 3 is a glycoprotein with a mass of approximately 58 kDa. This protein is mainly synthesized in the liver and circulates in the blood plasma. Antithrombin III functions as a serine protease inhibitor targeting and inhibiting several key enzymes involved in blood coagulation including thrombin and Factor Xa. Its activity is influenced by the presence of heparin which increases its inhibitory effects on these enzymes. This interaction is essential for regulating blood clot formation and maintaining hemostatic balance.

Biological function summary

Antithrombin III plays a critical role in blood clotting by specifically inhibiting clotting factors. It forms complexes with its target enzymes leading to their inactivation and preventing excessive thrombin production. The protein does not form part of larger complexes but acts independently to exert its anticoagulant function. The normal range for antithrombin 3 levels in plasma is important for ensuring a balanced blood coagulation system. Its function helps prevent inappropriate clot formation and ensures smooth blood flow through the circulatory system.

Pathways

Antithrombin III is integral to the coagulation cascade. It is primarily involved in the intrinsic and common coagulation pathways where it regulates thrombin and Factor Xa activity. This regulation prevents the unchecked formation of fibrin clots which can lead to thrombosis. ATIII works closely with proteins like heparin cofactor II and protein C which also modulate the coagulation pathway emphasizing its role in maintaining hemostatic balance. Its function is essential in controlling the coagulation cascade and ensuring that it proceeds appropriately without leading to pathology.

Antithrombin III deficiency can lead to coagulation disorders such as thrombophilia characterized by an increased risk of forming clots. Inherited or acquired deficiencies in antithrombin 3 can result in life-threatening thrombotic events. Furthermore liver diseases which affect the production of ATIII can also result in decreased antithrombin activity compounding coagulation problems. Other proteins related to this process include protein S and plasminogen which also play roles in clot regulation and fibrinolysis. Understanding the connection of antithrombin III to these pathways and diseases is important for developing effective therapeutic strategies.

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Product protocols

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Target data

Most important serine protease inhibitor in plasma that regulates the blood coagulation cascade (PubMed : 15140129, PubMed : 15853774). AT-III inhibits thrombin, matriptase-3/TMPRSS7, as well as factors IXa, Xa and XIa (PubMed : 15140129). Its inhibitory activity is greatly enhanced in the presence of heparin.

See full target information SERPINC1

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Bioactive materials 45:181-200 PubMed39651397

2024

Engineering aortic valves via transdifferentiating fibroblasts into valvular endothelial cells without using viruses or iPS cells.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Tang,Fuxiang Wei,Weihua Qiao,Xing Chen,Chenyang Ji,Wanzhi Yang,Xinyu Zhang,Sihan Chen,Yanyan Wu,Mingxing Jiang,Chenyu Ma,Weiqiang Shen,Qi Dong,Hong Cao,Minghui Xie,Ziwen Cai,Li Xu,Jiawei Shi,Nianguo Dong,Junwei Chen,Ning Wang

Frontiers in neurology 13:886040 PubMed36003300

2022

Identification of protein biomarkers in host cerebrospinal fluid for differential diagnosis of tuberculous meningitis and other meningitis.

Applications

Unspecified application

Species

Unspecified reactive species

Mailing Huang,Zeyu Ding,Wensheng Li,Weibi Chen,Yadong Du,Hongyan Jia,Qi Sun,Boping Du,Rongrong Wei,Aiying Xing,Qi Li,Naihui Chu,Liping Pan

Scientific reports 11:21852 PubMed34750441

2021

Human endothelial cells and fibroblasts express and produce the coagulation proteins necessary for thrombin generation.

Applications

Unspecified application

Species

Unspecified reactive species

Clay T Cohen,Nancy A Turner,Joel L Moake

View all publications

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websiteProtocolBooklet

en

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Product promise

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