Human Antithrombin III ELISA Kit
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Human Antithrombin III ELISA Kit is a Competitive ELISA for the measurement of Human Antithrombin III in Human in Biofluids samples.
View Alternative Names
AT3, PRO0309, SERPINC1, Antithrombin-III, ATIII, Serpin C1
- cELISA
Supplier Data
Competitive ELISA - Human Antithrombin III ELISA Kit (AB220073)
Human ATIII Standard curve (ab220073)
Reactivity data
Product details
The Human Antithrombin III ELISA (Enzyme-Linked Immunosorbent Assay) kit (ab220073) is designed for detection of Human Antithrombin III (AT3) in plasma and serum samples.
This assay employs a quantitative competitive enzyme immunoassay technique that measures Antithrombin III in less than 3 hours. A polyclonal antibody specific for Antithrombin III has been pre-coated onto a 96-well microplate with removable strips. Antithrombin III in standards and samples compete with a biotinylated Antithrombin III sandwiched by the immobilized antibody and streptavidin-peroxidase conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
The serine protease inhibitor antithrombin III, the most important natural inhibitor of thrombin activity, has been shown to exert marked anti-inflammatory properties. AT3 levels are positively correlated with plasma total cholesterol levels, plasma low-density lipoprotein cholesterol levels, plasma triglycerides and D-dimer levels.
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Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
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Biological function summary
Antithrombin III plays a critical role in blood clotting by specifically inhibiting clotting factors. It forms complexes with its target enzymes leading to their inactivation and preventing excessive thrombin production. The protein does not form part of larger complexes but acts independently to exert its anticoagulant function. The normal range for antithrombin 3 levels in plasma is important for ensuring a balanced blood coagulation system. Its function helps prevent inappropriate clot formation and ensures smooth blood flow through the circulatory system.
Pathways
Antithrombin III is integral to the coagulation cascade. It is primarily involved in the intrinsic and common coagulation pathways where it regulates thrombin and Factor Xa activity. This regulation prevents the unchecked formation of fibrin clots which can lead to thrombosis. ATIII works closely with proteins like heparin cofactor II and protein C which also modulate the coagulation pathway emphasizing its role in maintaining hemostatic balance. Its function is essential in controlling the coagulation cascade and ensuring that it proceeds appropriately without leading to pathology.
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