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Human APLP1 ELISA Kit - Extracellular is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human APLP1 - Extracellular in Cerebral Spinal Fluid, Cell culture supernatant, Heparin Plasma, EDTA Plasma, Citrate plasma, Serum samples.

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Images

Sandwich ELISA - Human APLP1 ELISA Kit -  Extracellular (AB309112), expandable thumbnail
  • Sandwich ELISA - Human APLP1 ELISA Kit -  Extracellular (AB309112), expandable thumbnail
  • Sandwich ELISA - Human APLP1 ELISA Kit -  Extracellular (AB309112), expandable thumbnail
  • Sandwich ELISA - Human APLP1 ELISA Kit -  Extracellular (AB309112), expandable thumbnail

Key facts

Detection method

Colorimetric

Sample types

Cerebral Spinal Fluid, Cell culture supernatant, Heparin Plasma, EDTA Plasma, Citrate plasma, Serum

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

23.438 - 1500 pg/mL

Assay time

1h 30m

Sensitivity

= 7.5 pg/mL

Reactivity data

Application

sELISA

Reactivity

Reacts

Dilution info
-
Notes

-

Target data

Function

May play a role in postsynaptic function. The C-terminal gamma-secretase processed fragment, ALID1, activates transcription activation through APBB1 (Fe65) binding (By similarity). Couples to JIP signal transduction through C-terminal binding. May interact with cellular G-protein signaling pathways. Can regulate neurite outgrowth through binding to components of the extracellular matrix such as heparin and collagen I.The gamma-CTF peptide, C30, is a potent enhancer of neuronal apoptosis.

Alternative names

What's included?

1 x 96 Tests
Components
10X Human APLP1 (Extracellular) Capture Antibody
1 x 600 µL
10X Human APLP1 (Extracellular) Detector Antibody
1 x 600 µL
10X Wash Buffer PT (ab206977)
1 x 20 mL
Antibody Diluent CPI2
1 x 6 mL
Human APLP1 (Extracellular) Lyophilized Recombinant Protein
2 x 1 Vial
Plate Seals
1 x 1 Unit
Sample Diluent NS (ab193972)
1 x 50 mL
SimpleStep Pre-Coated 96-Well Microplate (ab206978)
1 x 1 Unit
Stop Solution
1 x 12 mL
TMB Development Solution
1 x 12 mL

Recommended products

Human APLP1 ELISA Kit - Extracellular is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human APLP1 - Extracellular in Cerebral Spinal Fluid, Cell culture supernatant, Heparin Plasma, EDTA Plasma, Citrate plasma, Serum samples.

Key facts

Detection method

Colorimetric

Sample types

Cerebral Spinal Fluid, Cell culture supernatant, Heparin Plasma, EDTA Plasma, Citrate plasma, Serum

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

23.438 - 1500 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

Sensitivity

= 7.5 pg/mL

General recovery

104%

Precision

Intra assay

Sample

Serum

n
8
mean
-
SD
-
C.V.

9.6

Inter assay

Sample

Serum

n
3
mean
-
SD
-
C.V.

3.4

Recovery

Sample specific recovery

Sample type

Serum

Average %

= 104

Range

99 - 111 %

Sample type

Citrate plasma

Average %

= 104

Range

84 - 117 %

Sample type

EDTA Plasma

Average %

= 92

Range

79.2 - 100 %

Sample type

Heparin Plasma

Average %

= 89

Range

82 - 94 %

Sample type

Cell culture supernatant

Average %

= 95

Range

88 - 105 %

Sample type

Cerebral Spinal Fluid

Average %

= 99

Range

98 - 100 %

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

Human APLP1 (Extracellular) SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of APLP1 (Extracellular) protein in human serum, plasma, saliva and cell culture supernatant. Quantitate Human APLP1 (Extracellular) with 7.5 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

APLP1 also known as amyloid precursor-like protein 1 is a protein with a molecular mass of approximately 91 kDa. Researchers have observed its expression in the brain particularly in regions like the cortex and hippocampus. This protein shares structural similarities with the Amyloid Precursor Protein (APP) family and plays important roles in cellular functions through its protein domains and motifs.

Biological function summary

APLP1 functions by contributing to cell-cell adhesion and synaptic formation impacting neuronal development. It forms part of a larger protein complex that modulates these processes interacting with other proteins to maintain cellular communication. This protein's activity supports the stability and plasticity of synapses which is essential for proper neural network function.

Pathways

Proteins related to APLP1 participate notably in the Notch signaling and MAPK pathways. These pathways influence cell fate decisions growth and survival. APLP1 interacts with proteins such as APP and APLP2 both of which also engage in similar signaling pathways. This interconnectedness highlights APLP1's role in neurobiological processes essential for normal brain operation.

Associated diseases and disorders

Researchers link APLP1 to neurodegenerative disorders like Alzheimer's disease and autism spectrum disorders. Changes in APLP1 expression or function can affect amyloidogenic processing connecting it to APP and potentially leading to plaque formation a hallmark of Alzheimer's disease. Additionally this protein's relationship with synaptic function may contribute to the pathophysiology observed in autism spectrum disorders where alterations in synaptic communication are common.

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4 product images

  • Sandwich ELISA - Human APLP1 ELISA Kit -  Extracellular (ab309112), expandable thumbnail

    Sandwich ELISA - Human APLP1 ELISA Kit - Extracellular (ab309112)

    Interpolated concentration of native APLP1 was measured in duplicate at different sample concentrations. Undiluted samples are as follows: SH-SY5Y cell culture supernatant 25%, CSF 1:500.  The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS. 

  • Sandwich ELISA - Human APLP1 ELISA Kit -  Extracellular (ab309112), expandable thumbnail

    Sandwich ELISA - Human APLP1 ELISA Kit - Extracellular (ab309112)

    Example of human APLP1 standard curve. Background-subtracted data values (mean +/- SD) are graphed. 

  • Sandwich ELISA - Human APLP1 ELISA Kit -  Extracellular (ab309112), expandable thumbnail

    Sandwich ELISA - Human APLP1 ELISA Kit - Extracellular (ab309112)

    Serum of ten individual healthy human male donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean APLP1 concentration was determined to be 10,190 pg/mL with a range of 5,923 – 18,049 pg/mL. 

  • Sandwich ELISA - Human APLP1 ELISA Kit -  Extracellular (ab309112), expandable thumbnail

    Sandwich ELISA - Human APLP1 ELISA Kit - Extracellular (ab309112)

    Interpolated concentration of native APLP1 was measured in duplicate at different sample concentrations. Undiluted samples are as follows: serum 12%, plasma (citrate) 15%, plasma (EDTA) 25%, and plasma (heparin) 16%.  The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS. 

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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