AB309112

Human APLP1 ELISA Kit - Extracellular

SimpleStep
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Human APLP1 ELISA Kit - Extracellular is a single-wash 90-min Simplestep used to quantify Human APLP1 - Extracellular with a sensitivity of 7.5 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Validated on a number of sample types including cerebrospinal fluid (CSF)
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Amyloid beta precursor like protein 1, Amyloid beta (A4) precursor-like protein 1, Amyloid-like protein 1, APLP, APLP-1, APLP1

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Sandwich ELISA - Human APLP1 ELISA Kit - Extracellular (AB309112)

sELISA

Supplier Data

Sandwich ELISA - Human APLP1 ELISA Kit - Extracellular (AB309112)

Interpolated concentration of native APLP1 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : serum 12%, plasma (citrate) 15%, plasma (EDTA) 25%, and plasma (heparin) 16%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Human APLP1 ELISA Kit - Extracellular (AB309112)

Example of human APLP1 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human APLP1 ELISA Kit - Extracellular (AB309112)

Serum of ten individual healthy human male donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean APLP1 concentration was determined to be 10,190 pg/mL with a range of 5,923 – 18,049 pg/mL.

sELISA

Supplier Data

Sandwich ELISA - Human APLP1 ELISA Kit - Extracellular (AB309112)

Interpolated concentration of native APLP1 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : SH-SY5Y cell culture supernatant 25%, CSF 1 : 500. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Key facts

Detection method

Colorimetric

Sample types

Cerebral Spinal Fluid, Cell culture supernatant, Heparin Plasma, EDTA Plasma, Citrate plasma, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 7.5 pg/mL

Range

23.438 - 1500 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human APLP1 (Extracellular) SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of APLP1 (Extracellular) protein in human serum, plasma, saliva and cell culture supernatant. Quantitate Human APLP1 (Extracellular) with 7.5 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9.6" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.4" } ]

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Recovery

[ { "sample": "Serum", "range": "99 - 111 %", "average": "= 104" }, { "sample": "Citrate plasma", "range": "84 - 117 %", "average": "= 104" }, { "sample": "EDTA Plasma", "range": "79.2 - 100 %", "average": "= 92" }, { "sample": "Heparin Plasma", "range": "82 - 94 %", "average": "= 89" }, { "sample": "Cell culture supernatant", "range": "88 - 105 %", "average": "= 95" }, { "sample": "Cerebral Spinal Fluid", "range": "98 - 100 %", "average": "= 99" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

APLP1 also known as amyloid precursor-like protein 1 is a protein with a molecular mass of approximately 91 kDa. Researchers have observed its expression in the brain particularly in regions like the cortex and hippocampus. This protein shares structural similarities with the Amyloid Precursor Protein (APP) family and plays important roles in cellular functions through its protein domains and motifs.

Biological function summary

APLP1 functions by contributing to cell-cell adhesion and synaptic formation impacting neuronal development. It forms part of a larger protein complex that modulates these processes interacting with other proteins to maintain cellular communication. This protein's activity supports the stability and plasticity of synapses which is essential for proper neural network function.

Pathways

Proteins related to APLP1 participate notably in the Notch signaling and MAPK pathways. These pathways influence cell fate decisions growth and survival. APLP1 interacts with proteins such as APP and APLP2 both of which also engage in similar signaling pathways. This interconnectedness highlights APLP1's role in neurobiological processes essential for normal brain operation.

Researchers link APLP1 to neurodegenerative disorders like Alzheimer's disease and autism spectrum disorders. Changes in APLP1 expression or function can affect amyloidogenic processing connecting it to APP and potentially leading to plaque formation a hallmark of Alzheimer's disease. Additionally this protein's relationship with synaptic function may contribute to the pathophysiology observed in autism spectrum disorders where alterations in synaptic communication are common.

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Product protocols

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Target data

May play a role in postsynaptic function. The C-terminal gamma-secretase processed fragment, ALID1, activates transcription activation through APBB1 (Fe65) binding (By similarity). Couples to JIP signal transduction through C-terminal binding. May interact with cellular G-protein signaling pathways. Can regulate neurite outgrowth through binding to components of the extracellular matrix such as heparin and collagen I.. The gamma-CTF peptide, C30, is a potent enhancer of neuronal apoptosis.

See full target information APLP1

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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