Human APLP1 ELISA Kit - Intracellular is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human APLP1 - Intracellular in Cell Lysate, Tissue Extracts samples.
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell Lysate, Tissue Extracts
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 21.094 - 1350 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 4.5 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
May play a role in postsynaptic function. The C-terminal gamma-secretase processed fragment, ALID1, activates transcription activation through APBB1 (Fe65) binding (By similarity). Couples to JIP signal transduction through C-terminal binding. May interact with cellular G-protein signaling pathways. Can regulate neurite outgrowth through binding to components of the extracellular matrix such as heparin and collagen I. The gamma-CTF peptide, C30, is a potent enhancer of neuronal apoptosis.
Alternative names
Amyloid beta precursor like protein 1, Amyloid beta (A4) precursor-like protein 1, Amyloid-like protein 1, APLP, APLP-1, APLP1
What's included?
Recommended products
Human APLP1 ELISA Kit - Intracellular is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human APLP1 - Intracellular in Cell Lysate, Tissue Extracts samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell Lysate, Tissue Extracts
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 21.094 - 1350 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 4.5 pg/mL
- General recovery
- 104%
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Extract | n 8 | mean - | SD - | C.V. 7 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Extract | n 3 | mean - | SD - | C.V. 5.5 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Tissue Extracts | Average % = 104 | Range 100 - 107 % |
Sample type Cell Lysate | Average % = 110 | Range 100 - 120 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human APLP1 (Intracellular) SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of APLP1 (Intracellular) protein in human cell lysate and tissue extract samples. Quantitate Human APLP1 (Intracellular) with 4.5 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (® kits.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
APLP1 also known as amyloid precursor-like protein 1 is a protein with a molecular mass of approximately 91 kDa. Researchers have observed its expression in the brain particularly in regions like the cortex and hippocampus. This protein shares structural similarities with the Amyloid Precursor Protein (APP) family and plays important roles in cellular functions through its protein domains and motifs.
- Biological function summary
APLP1 functions by contributing to cell-cell adhesion and synaptic formation impacting neuronal development. It forms part of a larger protein complex that modulates these processes interacting with other proteins to maintain cellular communication. This protein's activity supports the stability and plasticity of synapses which is essential for proper neural network function.
- Pathways
Proteins related to APLP1 participate notably in the Notch signaling and MAPK pathways. These pathways influence cell fate decisions growth and survival. APLP1 interacts with proteins such as APP and APLP2 both of which also engage in similar signaling pathways. This interconnectedness highlights APLP1's role in neurobiological processes essential for normal brain operation.
- Associated diseases and disorders
Researchers link APLP1 to neurodegenerative disorders like Alzheimer's disease and autism spectrum disorders. Changes in APLP1 expression or function can affect amyloidogenic processing connecting it to APP and potentially leading to plaque formation a hallmark of Alzheimer's disease. Additionally this protein's relationship with synaptic function may contribute to the pathophysiology observed in autism spectrum disorders where alterations in synaptic communication are common.
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3 product images
Sandwich ELISA - Human APLP1 ELISA Kit - Intracellular (ab309110)
Example of human APLP1 standard curve. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human APLP1 ELISA Kit - Intracellular (ab309110)
Human, rat, and mouse brain tissue extract samples were measured in duplicate at different sample concentrations. Undiluted samples are 2.5 µg/mL human brain cortex, rat whole brain, and mouse whole brain tissue extracts. The interpolated (using human protein standard) dilution factor corrected values in pg per microgram of tissue extract are plotted (mean +/- SD, n=2).
Sandwich ELISA - Human APLP1 ELISA Kit - Intracellular (ab309110)
Interpolated concentration of native APLP1 was measured in duplicate at different sample concentrations. Undiluted samples are as follows: SH-SY5Y 112.5 µg/mL and brain cortex tissue extract 2.5 µg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.
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