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AB315780

Human Apo-D ELISA Kit

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Human Apo-D ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Apo-D with a sensitivity of 6.008 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Apolipoprotein D, Apo-D, ApoD, APOD

5 Images
Sandwich ELISA - Human Apo-D ELISA Kit (AB315780)
  • sELISA

Supplier Data

Sandwich ELISA - Human Apo-D ELISA Kit (AB315780)

Serum of ten individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Apo-D concentration was determined to be 143.4 µg/mL with a range of 71.9 – 186.1 µg/mL.

Sandwich ELISA - Human Apo-D ELISA Kit (AB315780)
  • sELISA

Supplier Data

Sandwich ELISA - Human Apo-D ELISA Kit (AB315780)

Interpolated concentration of native Apo-D was measured in duplicate at different sample concentrations. Undiluted samples are as follows : 1 : 80,000 serum, 1 : 40,000 plasma (citrate), 1 : 80,000 plasma (EDTA), and 1 : 80,000 plasma (heparin).  The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human Apo-D ELISA Kit (AB315780)
  • sELISA

Supplier Data

Sandwich ELISA - Human Apo-D ELISA Kit (AB315780)

Interpolated concentration of native Apo-D was measured in duplicate at different sample concentrations. Undiluted samples are as follows : 100% A431 cell culture supernatant.  The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human Apo-D ELISA Kit (AB315780)
  • sELISA

Supplier Data

Sandwich ELISA - Human Apo-D ELISA Kit (AB315780)

Example of human Apo-D standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Apo-D ELISA Kit (AB315780)
  • sELISA

Supplier Data

Sandwich ELISA - Human Apo-D ELISA Kit (AB315780)

Interpolated concentration of native Apo-D was measured in duplicate at different sample concentrations. Undiluted samples are as follows : 1 : 800 urine.  The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Key facts

Detection method

Colorimetric

Sample types

Serum, EDTA Plasma, Citrate plasma, Heparin Plasma, Urine, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 6.008 pg/mL

Range

25 - 1600 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Apo-D SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Apo-D protein in Serum, EDTA Plasma, Cit Plasma, Hep Plasma, Urine, Cell culture supernatant . Quantitate Human Apo-D with 6.008 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9.8" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.1" } ]
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Recovery

[ { "sample": "Serum", "range": "92 - 117 %", "average": "= 105" }, { "sample": "EDTA Plasma", "range": "79 - 107 %", "average": "= 89" }, { "sample": "Citrate plasma", "range": "86 - 103 %", "average": "= 96" }, { "sample": "Heparin Plasma", "range": "98 - 118 %", "average": "= 109" }, { "sample": "Urine", "range": "84 - 88 %", "average": "= 86" }, { "sample": "Cell culture media", "range": "100 - 117 %", "average": "= 108" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Apo-D also known as apolipoprotein D is a glycoprotein with an approximate mass of 29 kilodaltons. As an apolipoprotein Apo-D is part of the lipocalin family which participates in the transport of small hydrophobic molecules. It is widely expressed in human tissues particularly in the brain liver and adrenal glands. The expression of Apo-D in these tissues suggests important roles in diverse physiological processes and lipid metabolism. Scientists often study Apo-D to understand its involvement in lipid transport and its biological activities.
Biological function summary

Apo-D interacts with various molecules affecting lipid and cholesterol transport and metabolism. Apo-D operates as part of a protein complex and binds ligands such as progesterone and arachidonic acid. These interactions suggest it might modulate their availability and activity in biological systems. Furthermore the role of Apo-D in protecting cells against oxidative stress emerges as an important mechanism of action contributing to its protective properties in neural and other tissues.

Pathways

Apo-D plays significant roles by influencing the HDL (high-density lipoprotein) metabolism pathway and the arachidonic acid pathway. Within the HDL pathway Apo-D associates with other apolipoproteins like apoA-I to regulate cholesterol transport and anti-inflammatory responses. Its involvement in the arachidonic acid pathway suggests a possible regulatory effect on inflammation and cellular stress responses highlighting the complex interaction of Apo-D with other proteins like cyclooxygenases which are key players in inflammation.

Researchers have linked Apo-D to conditions such as Alzheimer's disease and schizophrenia. In Alzheimer's disease its altered expression in the brain suggests a protective response or involvement in disease progression possibly through interaction with amyloid precursor proteins or influencing cholesterol metabolism. In schizophrenia abnormal levels of Apo-D in cerebrospinal fluid mark potential disturbance in lipid metabolism making it a protein of interest in the disease's complex pathophysiology. Understanding these connections provides insight into therapeutic strategies that target the biological functions of Apo-D.
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Product protocols

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Target data

APOD occurs in the macromolecular complex with lecithin-cholesterol acyltransferase. It is probably involved in the transport and binding of bilin. Appears to be able to transport a variety of ligands in a number of different contexts.
See full target information Apolipoprotein D
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websiteProtocolBooklet
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Complementary Products

Primary Antibodies

AB256496

Anti-Apo-D antibody [EPR22967-72]

RabMAb|Recombinant

Western blot - Anti-Apo-D antibody [EPR22967-72] (AB256496)

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Proteins & Peptides

AB184598

Recombinant Human Apo-D protein

SDS-PAGE - Recombinant Human Apo-D protein (AB184598)

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