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AB309168

Human APOA1 ELISA Kit

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Human APOA1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human APOA1 with a sensitivity of 73.07 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader

View Alternative Names

Apolipoprotein A-I, Apo-AI, ApoA-I, Apolipoprotein A1, APOA1

5 Images
Sandwich ELISA - Human APOA1 ELISA Kit (AB309168)
  • sELISA

Supplier Data

Sandwich ELISA - Human APOA1 ELISA Kit (AB309168)

Interpolated concentration of native APOA1 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : serum 1 : 600,000 and HepG2 supernatant 1 : 800.  The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS

Sandwich ELISA - Human APOA1 ELISA Kit (AB309168)
  • sELISA

Supplier Data

Sandwich ELISA - Human APOA1 ELISA Kit (AB309168)

Standard curve comparison between the original Human APOA1 SimpleStep ELISA (ab189576) and current Human APOA1 SimpleStep ELISA (ab309168).

Sandwich ELISA - Human APOA1 ELISA Kit (AB309168)
  • sELISA

Supplier Data

Sandwich ELISA - Human APOA1 ELISA Kit (AB309168)

Standard curve comparison between Human APOA1 SimpleStep ELISA kit (ab309168) and traditional ELISA kit from leading competitor. The SimpleStep ELISA kit shows increased sensitivity.

Sandwich ELISA - Human APOA1 ELISA Kit (AB309168)
  • sELISA

Supplier Data

Sandwich ELISA - Human APOA1 ELISA Kit (AB309168)

Serum of ten individual healthy human male donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +- SD, n=2). The mean APOA1 concentration was determined to be 3.04 mg/mL with a range of 1.65 – 5.61 mg/mL

Sandwich ELISA - Human APOA1 ELISA Kit (AB309168)
  • sELISA

Supplier Data

Sandwich ELISA - Human APOA1 ELISA Kit (AB309168)

Example of human APOA1 standard curve. Background-subtracted data values (mean +/- SD) are graphed

Key facts

Detection method

Colorimetric

Sample types

Serum, Cell culture supernatant, Urine

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 73.07 pg/mL

Range

281.25 - 18000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human APOA1 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of APOA1 protein in human serum, cell culture superrnatant and urine. Quantitate Human APOA1 with 73.07 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.2" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "0.8" } ]
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Recovery

[ { "sample": "Serum", "range": "93 - 119 %", "average": "= 107" }, { "sample": "Cell culture supernatant", "range": "95 - 109 %", "average": "= 103" }, { "sample": "Urine", "range": "87 - 91 %", "average": "= 89" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab309168-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB309168-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB309168-CMP08", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB309168-CMP05", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB309168-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB309168-CMP07", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB309168-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human APOA1 Capture Antibody", "number":"AB309168-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human APOA1 Lyophilized Recombinant Protein", "number":"AB309168-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB309168-CMP10", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human APOA1 Detector Antibody", "number":"AB309168-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Apolipoprotein A-I (ApoA-I) is a major component of high-density lipoprotein (HDL) particles often referred to as 'good cholesterol'. It has a molecular mass of approximately 28 kDa. ApoA-I is mainly expressed in the liver and intestine. It plays a mechanical role in the reverse transport of cholesterol acting to facilitate the efflux of cholesterol from tissues to the liver for excretion. Its alternative names include ApoAI ApoA1 and a component of the AI kits used for measuring this protein.
Biological function summary

ApoA-I functions in cholesterol homeostasis and inflammation. It is a structural component of the HDL complex that mobilizes cholesterol. ApoA-I acts as an activator of the enzyme lecithin-cholesterol acyltransferase (LCAT) which is essential for the maturation of HDL particles. This maturation is necessary for effective cholesterol transport and clearance. ApoA-I's ability to stabilize HDL particles and enhance their functionality makes it significant for maintaining lipid balance and cellular homeostasis.

Pathways

The interaction of ApoA-I with HDL formation and function marks its role in lipid metabolism pathways. Its participation in the reverse cholesterol transport pathway highlights its influence on cardiovascular health. ApoA-I also interacts with other proteins like ApoA-II and paraoxonase-1 which further influence lipid metabolism and antioxidant activities. Understanding these relationships helps elucidate the dynamics of cholesterol removal from the bloodstream.

Disturbances in ApoA-I levels correlate with cardiovascular disease and atherosclerosis. Deficiency or dysfunction in ApoA-I can impair HDL function leading to poor cholesterol removal and buildup within arteries. It is also connected to amyloidosis where misfolded ApoA-I forms deposits in tissues. Understanding these pathological conditions helps researchers target ApoA-I in therapeutic strategies to mitigate disease progression often studying it alongside proteins like ApoB which is associated with low-density lipoprotein (LDL) particles.
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Product protocols

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Target data

Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.
See full target information APOA1
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websiteProtocolBooklet
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Product promise

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