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AB189576

Human Apolipoprotein AI ELISA Kit

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(9 Publications)

Human Apolipoprotein AI ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Apolipoprotein AI with a sensitivity of 59 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders

View Alternative Names

Apolipoprotein A-I, Apo-AI, ApoA-I, Apolipoprotein A1, APOA1

5 Images
ELISA - Human Apolipoprotein AI ELISA Kit (AB189576)
  • ELISA

Supplier Data

ELISA - Human Apolipoprotein AI ELISA Kit (AB189576)
Sandwich ELISA - Human Apolipoprotein AI ELISA Kit (AB189576)
  • sELISA

Supplier Data

Sandwich ELISA - Human Apolipoprotein AI ELISA Kit (AB189576)

Titration of Human serum and plasma (citrate).

Human serum and plasma (citrate) diluted 2.5x105 –fold to 8x106 –fold in Sample Diluent NS. Background subtracted data from duplicate measurements are plotted.

Sandwich ELISA - Human Apolipoprotein AI ELISA Kit (AB189576)
  • sELISA

Supplier Data

Sandwich ELISA - Human Apolipoprotein AI ELISA Kit (AB189576)

Example of Apolipoprotein A1 standard curve.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Apolipoprotein AI ELISA Kit (AB189576)
  • sELISA

Supplier Data

Sandwich ELISA - Human Apolipoprotein AI ELISA Kit (AB189576)

Interpolated concentrations of Apolipoprotein A1 in Human serum from 10 donors. Serum from 10 apparently healthy male donors was measured in duplicate. The mean Apolipoprotein A1 concentration was determined to be 526 μg/mL with a range of 9.8-2011 μg/mL.

Sandwich ELISA - Human Apolipoprotein AI ELISA Kit (AB189576)
  • sELISA

Supplier Data

Sandwich ELISA - Human Apolipoprotein AI ELISA Kit (AB189576)

Interpolated concentrations of Apolipoprotein A1 in Human serum and plasma heparin. The concentrations of Apolipoprotein A1 were measured in duplicate and interpolated from the Apolipoprotein A1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Apolipoprotein A1 concentration was determined to be 2500 μg/mL in serum and 1053ug/mL in plasma heparin.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 59 pg/mL

Range

0.313 - 20 ng/mL

Assay time

1h 30m

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Apolipoprotein AI ELISA Kit ab189576 is a rapid single-wash 90-min Sandwich ELISA to measure Human Apolipoprotein AI in cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 59 pg/mL.

How the assay works

Human Apolipoprotein AI SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human Apolipoprotein AI ELISA Kit ab189576 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Apolipoprotein AI (ApoA1) is secreted by the liver and small intestine and is a major protein of plasma HDL (high density lipoprotein). APOA-I participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). Defects in APOA1I are associated with several diseases associated with low HDL levels (HDLD1 and HDLD2) and amyloidosis (AMYL8).

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.9" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.1" } ]
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Recovery

[ { "sample": "Serum", "range": "111 - 122 %", "average": "= 115" }, { "sample": "EDTA Plasma", "range": "108 - 115 %", "average": "= 112" }, { "sample": "Heparin Plasma", "range": "76 - 111 %", "average": "= 95" }, { "sample": "Citrate plasma", "range": "117 - 123 %", "average": "= 119" }, { "sample": "Tissue Culture Media", "range": "85 - 92 %", "average": "= 89" } ]
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What's included?

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab189576-10x96Tests", "assetComponentDetails": [ { "size":"1 x 120 mL", "name":"Stop Solution", "number":"AB189576-CMP07" }, { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB189576-CMP10" }, { "size":"2 x 250 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB189576-CMP05" }, { "size":"20 x 1 Vial", "name":"Human APOA1 Lyophilized Recombinant Protein", "number":"AB189576-CMP04" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB189576-CMP03" }, { "size":"10 x 600 µL", "name":"10X Human APOA1 Capture Antibody", "number":"AB189576-CMP01" }, { "size":"10 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB189576-CMP06" }, { "size":"10 x 600 µL", "name":"10X Human APOA1 Detector Antibody", "number":"AB189576-CMP02" }, { "size":"10 x 1 Unit", "name":"Plate Seals", "number":"AB189576-CMP09" }, { "size":"1 x 120 mL", "name":"TMB Development Solution", "number":"AB189576-CMP08" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab189576-1x96Tests", "assetComponentDetails": [ { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB189576-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB189576-CMP07", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB189576-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB189576-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB189576-CMP09", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB189576-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human APOA1 Lyophilized Recombinant Protein", "number":"AB189576-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human APOA1 Capture Antibody", "number":"AB189576-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB189576-CMP06", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human APOA1 Detector Antibody", "number":"AB189576-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Apolipoprotein A-I (ApoA-I) is a major component of high-density lipoprotein (HDL) particles often referred to as 'good cholesterol'. It has a molecular mass of approximately 28 kDa. ApoA-I is mainly expressed in the liver and intestine. It plays a mechanical role in the reverse transport of cholesterol acting to facilitate the efflux of cholesterol from tissues to the liver for excretion. Its alternative names include ApoAI ApoA1 and a component of the AI kits used for measuring this protein.
Biological function summary

ApoA-I functions in cholesterol homeostasis and inflammation. It is a structural component of the HDL complex that mobilizes cholesterol. ApoA-I acts as an activator of the enzyme lecithin-cholesterol acyltransferase (LCAT) which is essential for the maturation of HDL particles. This maturation is necessary for effective cholesterol transport and clearance. ApoA-I's ability to stabilize HDL particles and enhance their functionality makes it significant for maintaining lipid balance and cellular homeostasis.

Pathways

The interaction of ApoA-I with HDL formation and function marks its role in lipid metabolism pathways. Its participation in the reverse cholesterol transport pathway highlights its influence on cardiovascular health. ApoA-I also interacts with other proteins like ApoA-II and paraoxonase-1 which further influence lipid metabolism and antioxidant activities. Understanding these relationships helps elucidate the dynamics of cholesterol removal from the bloodstream.

Disturbances in ApoA-I levels correlate with cardiovascular disease and atherosclerosis. Deficiency or dysfunction in ApoA-I can impair HDL function leading to poor cholesterol removal and buildup within arteries. It is also connected to amyloidosis where misfolded ApoA-I forms deposits in tissues. Understanding these pathological conditions helps researchers target ApoA-I in therapeutic strategies to mitigate disease progression often studying it alongside proteins like ApoB which is associated with low-density lipoprotein (LDL) particles.
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Product protocols

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Target data

Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.
See full target information APOA1
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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

FEBS letters 599:1055-1074 PubMed39739361

2025

Insights into the renal pathophysiology in Hermansky-Pudlak syndrome-1 from urinary extracellular vesicle proteomics and a new mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Dawn M Maynard,Bernadette R Gochuico,Hadass Pri Chen,Christopher K E Bleck,Patricia M Zerfas,Wendy J Introne,William A Gahl,May C V Malicdan

eLife 12: PubMed38787378

2024

Secreted dengue virus NS1 from infection is predominantly dimeric and in complex with high-density lipoprotein.

Applications

Unspecified application

Species

Unspecified reactive species

Bing Liang Alvin Chew,A N Qi Ngoh,Wint Wint Phoo,Kitti Wing Ki Chan,Zheng Ser,Nikhil K Tulsian,Shiao See Lim,Mei Jie Grace Weng,Satoru Watanabe,Milly M Choy,Jenny Low,Eng Eong Ooi,Christiane Ruedl,Radoslaw M Sobota,Subhash G Vasudevan,Dahai Luo

Frontiers in neurology 13:886040 PubMed36003300

2022

Identification of protein biomarkers in host cerebrospinal fluid for differential diagnosis of tuberculous meningitis and other meningitis.

Applications

Unspecified application

Species

Unspecified reactive species

Mailing Huang,Zeyu Ding,Wensheng Li,Weibi Chen,Yadong Du,Hongyan Jia,Qi Sun,Boping Du,Rongrong Wei,Aiying Xing,Qi Li,Naihui Chu,Liping Pan

Frontiers in immunology 13:855513 PubMed35677050

2022

Proteomic Profiling of Cryoglobulinemia.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Liu,Jianqiang Wu,Dandan Sun,Haolong Li,Zhihong Qi,Xiaoyue Tang,Wei Su,Yongzhe Li,Xuzhen Qin

Arteriosclerosis, thrombosis, and vascular biology 40:656-669 PubMed31852220

2019

Retinoids Repress Human Cardiovascular Cell Calcification With Evidence for Distinct Selective Retinoid Modulator Effects.

Applications

Unspecified application

Species

Unspecified reactive species

Maximillian A Rogers,Jiaohua Chen,Shriram Nallamshetty,Tan Pham,Shinji Goto,Jochen D Muehlschlegel,Peter Libby,Masanori Aikawa,Elena Aikawa,Jorge Plutzky

Journal of proteome research 18:3977-3984 PubMed31545048

2019

Site-Specific Glycoprofiles of HDL-Associated ApoE are Correlated with HDL Functional Capacity and Unaffected by Short-Term Diet.

Applications

Unspecified application

Species

Unspecified reactive species

Chenghao Zhu,Maurice Wong,Qiongyu Li,Lisa Sawrey-Kubicek,Elizabeth Beals,Chris H Rhodes,Romina Sacchi,Carlito B Lebrilla,Angela M Zivkovic

Atherosclerosis 286:88-96 PubMed31103880

2019

Identification of circular RNA Hsa_circ_0001879 and Hsa_circ_0004104 as novel biomarkers for coronary artery disease.

Applications

Unspecified application

Species

Unspecified reactive species

Laiyuan Wang,Chong Shen,Yanyu Wang,Tianyu Zou,Huijuan Zhu,Xiaomei Lu,Lin Li,Bin Yang,Jichun Chen,Shufeng Chen,Xiangfeng Lu,Dongfeng Gu

Atherosclerosis 253:171-177 PubMed27451002

2016

Short-term isocaloric fructose restriction lowers apoC-III levels and yields less atherogenic lipoprotein profiles in children with obesity and metabolic syndrome.

Applications

sELISA

Species

Human

Alejandro Gugliucci,Robert H Lustig,Russell Caccavello,Ayca Erkin-Cakmak,Susan M Noworolski,Viva W Tai,Michael J Wen,Kathleen Mulligan,Jean-Marc Schwarz

Journal of lipid research 56:635-43 PubMed25593328

2015

Macrophage apoAI protects against dyslipidemia-induced dermatitis and atherosclerosis without affecting HDL.

Applications

ELISA

Species

Human

Hagai Tavori,Yan Ru Su,Patricia G Yancey,Ilaria Giunzioni,Ashley J Wilhelm,John L Blakemore,Manal Zabalawi,MacRae F Linton,Mary G Sorci-Thomas,Sergio Fazio
View all publications
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