Human Apolipoprotein AI ELISA Kit (APOA1) is a Sandwich (quantitative) ELISA kit for the measurement of Human Apolipoprotein AI (APOA1) in Human in Cerebral Spinal Fluid, Milk, Plasma, Saliva, Serum, Urine samples.
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- PloS one 18:e02874632023Prospective outcome analysis of multiple sclerosis cases reveals candidate prognostic cerebrospinal fluid markers.Applications:Unspecified applicationReactive species:Unspecified reactive speciesElif Everest,Ugur Uygunoglu,Melih Tutuncu,Alper Bulbul,Umut Inci Onat,Mehmetcan Unal,Timucin Avsar,Sabahattin Saip,Ugur Bilge,Eda Tahir Turanli,Aksel SivaPubMed 37339131
- Molecular neurodegeneration 18:202023Cerebrospinal fluid lipoproteins inhibit α-synuclein aggregation by interacting with oligomeric species in seed amplification assays.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGiovanni Bellomo,Silvia Paciotti,Luis Concha-Marambio,Domenico Rizzo,Anna Lidia Wojdaƚa,Davide Chiasserini,Leonardo Gatticchi,Linda Cerofolini,Stefano Giuntini,Chiara Maria Giulia De Luca,Yihua Ma,Carly M Farris,Giuseppe Pieraccini,Sara Bologna,Marta Filidei,Enrico Ravera,Moreno Lelli,Fabio Moda,Marco Fragai,Lucilla Parnetti,Claudio LuchinatPubMed 37005644
- Frontiers in cell and developmental biology 8:3672020Analysis of Neat Biofluids Obtained During Cardiac Surgery Using Nanoparticle Tracking Analysis: Methodological Considerations.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAndrew I U Shearn et. al.PubMed 32528952
- Cell metabolism 29:475-487.e72018Interleukin-17 Drives Interstitial Entrapment of Tissue Lipoproteins in Experimental Psoriasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLi-Hao Huang,Bernd H Zinselmeyer,Chih-Hao Chang,Brian T Saunders,Andrew Elvington,Osamu Baba,Thomas J Broekelmann,Lina Qi,Joseph S Rueve,Melody A Swartz,Brian S Kim,Robert P Mecham,Helge Wiig,Michael J Thomas,Mary G Sorci-Thomas,Gwendalyn J RandolphPubMed 30415924
- The Journal of clinical endocrinology and metabolism 102:4117-41232017Adiponectin Predicts High-Density Lipoprotein Cholesterol Efflux Capacity in Adults Irrespective of Body Mass Index and Fat Distribution.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGunther Marsche,Sieglinde Zelzer,Andreas Meinitzer,Sabine Kern,Sabine Meissl,Gudrun Pregartner,Daniel Weghuber,Gunter Almer,Harald ManggePubMed 28938424
Key facts
- Detection method
- Colorimetric
- Sample types
- Cerebral Spinal Fluid, Milk, Plasma, Saliva, Serum, Urine
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 12.5 - 100 ng/mL
- Assay time
- 4h
- Sensitivity
- = 0.95 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.
Alternative names
Apolipoprotein A-I, Apo-AI, ApoA-I, Apolipoprotein A1, APOA1
What's included?
Recommended products
Human Apolipoprotein AI ELISA Kit (APOA1) is a Sandwich (quantitative) ELISA kit for the measurement of Human Apolipoprotein AI (APOA1) in Human in Cerebral Spinal Fluid, Milk, Plasma, Saliva, Serum, Urine samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Cerebral Spinal Fluid, Milk, Plasma, Saliva, Serum, Urine
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 12.5 - 100 ng/mL
- Assay time
- 4h
- Assay Platform
- Microplate
- Sensitivity
- = 0.95 ng/mL
- General recovery
- 98%
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. 5.6 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. 10.5 |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- Multi
- Storage information
- Please refer to protocols
Notes
Abcam's Apolipoprotein AI (APOA1) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of apolipoprotein AI concentrations in plasma, serum, urine, saliva, milk, CSF and cell culture samples.
An Apolipoprotein AI specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an Apolipoprotein AI specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Apolipoprotein AI captured in plate.
Get results in 90 minutes with Human Apolipoprotein AI ELISA Kit (Human Apolipoprotein AI ELISA Kit ab189576) from our SimpleStep ELISA® range.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Apolipoprotein A-I (ApoA-I) is a major component of high-density lipoprotein (HDL) particles often referred to as 'good cholesterol'. It has a molecular mass of approximately 28 kDa. ApoA-I is mainly expressed in the liver and intestine. It plays a mechanical role in the reverse transport of cholesterol acting to facilitate the efflux of cholesterol from tissues to the liver for excretion. Its alternative names include ApoAI ApoA1 and a component of the AI kits used for measuring this protein.
- Biological function summary
ApoA-I functions in cholesterol homeostasis and inflammation. It is a structural component of the HDL complex that mobilizes cholesterol. ApoA-I acts as an activator of the enzyme lecithin-cholesterol acyltransferase (LCAT) which is essential for the maturation of HDL particles. This maturation is necessary for effective cholesterol transport and clearance. ApoA-I's ability to stabilize HDL particles and enhance their functionality makes it significant for maintaining lipid balance and cellular homeostasis.
- Pathways
The interaction of ApoA-I with HDL formation and function marks its role in lipid metabolism pathways. Its participation in the reverse cholesterol transport pathway highlights its influence on cardiovascular health. ApoA-I also interacts with other proteins like ApoA-II and paraoxonase-1 which further influence lipid metabolism and antioxidant activities. Understanding these relationships helps elucidate the dynamics of cholesterol removal from the bloodstream.
- Associated diseases and disorders
Disturbances in ApoA-I levels correlate with cardiovascular disease and atherosclerosis. Deficiency or dysfunction in ApoA-I can impair HDL function leading to poor cholesterol removal and buildup within arteries. It is also connected to amyloidosis where misfolded ApoA-I forms deposits in tissues. Understanding these pathological conditions helps researchers target ApoA-I in therapeutic strategies to mitigate disease progression often studying it alongside proteins like ApoB which is associated with low-density lipoprotein (LDL) particles.
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2 product images
Sandwich ELISA - Human Apolipoprotein AI ELISA Kit (APOA1) (ab108803)
APOA1 measured in various samples showing quantity (microgram) per mL of tested sample.
Sandwich ELISA - Human Apolipoprotein AI ELISA Kit (APOA1) (ab108803)
Representative Standard Curve using ab108803
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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