Human Apolipoprotein AI ELISA Kit (APOA1) is a Competitive ELISA kit for the measurement of Human Apolipoprotein AI (APOA1) in Human in Plasma, Serum samples.
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Publications
- JCI insight 7:2022Serological profiling reveals hsa-miR-451a as a possible biomarker of anaphylaxis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWojciech Francuzik,Kristijan Pažur,Magdalena Dalke,Sabine Dölle-Bierke,Magda Babina,Margitta WormPubMed 35202004
- ACS chemical neuroscience 13:1030-10452022Fibrinogen and Complement Factor H Are Promising CSF Protein Biomarkers for Parkinson's Disease with Cognitive Impairment─A Proteomics-ELISA-Based Study.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAditi Naskar,Albert Stezin,Arpitha Dharmappa,Shantala Hegde,Mariamma Philip,Nitish Kamble,Jitender Saini,K Sandhya,Utpal Tatu,Ravi Yadav,Pramod Kumar Pal,Phalguni Anand AlladiPubMed 35200010
Key facts
- Detection method
Colorimetric
- Sample types
Plasma, Serum
- Assay type
Competitive
- Reactive species
Human
- Range
0.625 - 5 µg/mL
- Assay time
3h
- Sensitivity
= 0.12 µg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application cELISA | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
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Target data
Function
Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.
Alternative names
Apolipoprotein A-I, Apo-AI, ApoA-I, Apolipoprotein A1, APOA1
What's included?
Recommended products
Human Apolipoprotein AI ELISA Kit (APOA1) is a Competitive ELISA kit for the measurement of Human Apolipoprotein AI (APOA1) in Human in Plasma, Serum samples.
Alternative names
Apolipoprotein A-I, Apo-AI, ApoA-I, Apolipoprotein A1, APOA1
Key facts
- Detection method
Colorimetric
- Sample types
Plasma, Serum
- Assay type
Competitive
- Reactive species
Human
- Range
0.625 - 5 µg/mL
- Assay time
3h
- Assay Platform
Microplate
- Sensitivity
= 0.12 µg/mL
- General recovery
98%
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. 4.8 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. 10.1 |
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
Multi
- Storage information
Please refer to protocols
Notes
Apolipoprotein AI Human (APOA1) in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Apolipoprotein AI levels in plasma and serum.
An Apolipoprotein AI specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Apolipoprotein AI is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Apolipoprotein AI captured in plate.
Get results in 90 minutes with Human Apolipoprotein AI ELISA Kit (Human Apolipoprotein AI ELISA Kit ab189576) from our SimpleStep ELISA® range.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
Supplementary info
- Activity summary
Apolipoprotein A-I (ApoA-I) is a major component of high-density lipoprotein (HDL) particles often referred to as 'good cholesterol'. It has a molecular mass of approximately 28 kDa. ApoA-I is mainly expressed in the liver and intestine. It plays a mechanical role in the reverse transport of cholesterol acting to facilitate the efflux of cholesterol from tissues to the liver for excretion. Its alternative names include ApoAI ApoA1 and a component of the AI kits used for measuring this protein.
- Biological function summary
ApoA-I functions in cholesterol homeostasis and inflammation. It is a structural component of the HDL complex that mobilizes cholesterol. ApoA-I acts as an activator of the enzyme lecithin-cholesterol acyltransferase (LCAT) which is essential for the maturation of HDL particles. This maturation is necessary for effective cholesterol transport and clearance. ApoA-I's ability to stabilize HDL particles and enhance their functionality makes it significant for maintaining lipid balance and cellular homeostasis.
- Pathways
The interaction of ApoA-I with HDL formation and function marks its role in lipid metabolism pathways. Its participation in the reverse cholesterol transport pathway highlights its influence on cardiovascular health. ApoA-I also interacts with other proteins like ApoA-II and paraoxonase-1 which further influence lipid metabolism and antioxidant activities. Understanding these relationships helps elucidate the dynamics of cholesterol removal from the bloodstream.
- Associated diseases and disorders
Disturbances in ApoA-I levels correlate with cardiovascular disease and atherosclerosis. Deficiency or dysfunction in ApoA-I can impair HDL function leading to poor cholesterol removal and buildup within arteries. It is also connected to amyloidosis where misfolded ApoA-I forms deposits in tissues. Understanding these pathological conditions helps researchers target ApoA-I in therapeutic strategies to mitigate disease progression often studying it alongside proteins like ApoB which is associated with low-density lipoprotein (LDL) particles.
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2 product images
Competitive ELISA - Human Apolipoprotein AI ELISA Kit (APOA1) (ab108804)
Apolipoprotein A1 measured in biological fluids and cell culture medium with background signal subtracted (duplicates +/- SD).
Competitive ELISA - Human Apolipoprotein AI ELISA Kit (APOA1) (ab108804)
Representative Standard Curve using ab108804
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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