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AB108804

Human Apolipoprotein AI ELISA Kit (APOA1)

4

(3 Reviews)

|

(12 Publications)

Human Apolipoprotein AI ELISA Kit (APOA1) is a Competitive ELISA for the measurement of Human Apolipoprotein AI (APOA1) in Human in Biofluids samples.

View Alternative Names

Apolipoprotein A-I, Apo-AI, ApoA-I, Apolipoprotein A1, APOA1

2 Images
Competitive ELISA - Human Apolipoprotein AI ELISA Kit (APOA1) (AB108804)
  • cELISA

Supplier Data

Competitive ELISA - Human Apolipoprotein AI ELISA Kit (APOA1) (AB108804)

Representative Standard Curve using ab108804

Competitive ELISA - Human Apolipoprotein AI ELISA Kit (APOA1) (AB108804)
  • cELISA

Lab

Competitive ELISA - Human Apolipoprotein AI ELISA Kit (APOA1) (AB108804)

Apolipoprotein A1 measured in biological fluids and cell culture medium with background signal subtracted (duplicates +/- SD).

Key facts

Detection method

Colorimetric

Sample types

Plasma, Serum

Reacts with

Human

Assay type

Competitive

Sensitivity

= 0.12 µg/mL

Range

0.156 - 10 µg/mL

Assay time

3h

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "cELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Apolipoprotein AI Human (APOA1) in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Apolipoprotein AI levels in plasma and serum.

An Apolipoprotein AI specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Apolipoprotein AI is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Apolipoprotein AI captured in plate.

Get results in 90 minutes with Human Apolipoprotein AI ELISA Kit (ab189576) from our SimpleStep ELISA® range.

The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.1" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.8" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab108804-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Vial", "name":"2X Biotinylated Human Apolipoprotein AI (Lyophilized)", "number":"AB108804-CMP01", "productcode":"" }, { "size":"1 x 30 mL", "name":"20X Wash Buffer Concentrate", "number":"AB108804-CMP06", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Solution", "number":"AB108804-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Apolipoprotein AI Microplate (12 x 8 well strips)", "number":"AB108804-CMP04", "productcode":"" }, { "size":"1 x 7 mL", "name":"Chromogen Substrate", "number":"AB108804-CMP03", "productcode":"" }, { "size":"1 x 30 mL", "name":"10X Diluent N Concentrate", "number":"AB108804-CMP02", "productcode":"" }, { "size":"1 x 80 µL", "name":"100X Streptavidin-Peroxidase Conjugate", "number":"AB108804-CMP09", "productcode":"" }, { "size":"3 x 1 Unit", "name":"Sealing Tapes", "number":"AB108804-CMP07", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Apolipoprotein AI Standard", "number":"AB108804-CMP05", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Apolipoprotein A-I (ApoA-I) is a major component of high-density lipoprotein (HDL) particles often referred to as 'good cholesterol'. It has a molecular mass of approximately 28 kDa. ApoA-I is mainly expressed in the liver and intestine. It plays a mechanical role in the reverse transport of cholesterol acting to facilitate the efflux of cholesterol from tissues to the liver for excretion. Its alternative names include ApoAI ApoA1 and a component of the AI kits used for measuring this protein.
Biological function summary

ApoA-I functions in cholesterol homeostasis and inflammation. It is a structural component of the HDL complex that mobilizes cholesterol. ApoA-I acts as an activator of the enzyme lecithin-cholesterol acyltransferase (LCAT) which is essential for the maturation of HDL particles. This maturation is necessary for effective cholesterol transport and clearance. ApoA-I's ability to stabilize HDL particles and enhance their functionality makes it significant for maintaining lipid balance and cellular homeostasis.

Pathways

The interaction of ApoA-I with HDL formation and function marks its role in lipid metabolism pathways. Its participation in the reverse cholesterol transport pathway highlights its influence on cardiovascular health. ApoA-I also interacts with other proteins like ApoA-II and paraoxonase-1 which further influence lipid metabolism and antioxidant activities. Understanding these relationships helps elucidate the dynamics of cholesterol removal from the bloodstream.

Disturbances in ApoA-I levels correlate with cardiovascular disease and atherosclerosis. Deficiency or dysfunction in ApoA-I can impair HDL function leading to poor cholesterol removal and buildup within arteries. It is also connected to amyloidosis where misfolded ApoA-I forms deposits in tissues. Understanding these pathological conditions helps researchers target ApoA-I in therapeutic strategies to mitigate disease progression often studying it alongside proteins like ApoB which is associated with low-density lipoprotein (LDL) particles.
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Product protocols

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Target data

Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.
See full target information APOA1
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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

JCI insight 7: PubMed35202004

2022

Serological profiling reveals hsa-miR-451a as a possible biomarker of anaphylaxis.

Applications

Unspecified application

Species

Unspecified reactive species

Wojciech Francuzik,Kristijan Pažur,Magdalena Dalke,Sabine Dölle-Bierke,Magda Babina,Margitta Worm

ACS chemical neuroscience 13:1030-1045 PubMed35200010

2022

Fibrinogen and Complement Factor H Are Promising CSF Protein Biomarkers for Parkinson's Disease with Cognitive Impairment─A Proteomics-ELISA-Based Study.

Applications

Unspecified application

Species

Unspecified reactive species

Aditi Naskar,Albert Stezin,Arpitha Dharmappa,Shantala Hegde,Mariamma Philip,Nitish Kamble,Jitender Saini,K Sandhya,Utpal Tatu,Ravi Yadav,Pramod Kumar Pal,Phalguni Anand Alladi

EBioMedicine 75:103802 PubMed34990893

2022

Blood levels of adiponectin and IL-1Ra distinguish type 3c from type 2 diabetes: Implications for earlier pancreatic cancer detection in new-onset diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Lucy Oldfield,Anthony Evans,Rohith Gopala Rao,Claire Jenkinson,Tejpal Purewal,Eftychia E Psarelli,Usha Menon,John F Timms,Stephen P Pereira,Paula Ghaneh,William Greenhalf,Christopher Halloran,Eithne Costello

Antioxidants (Basel, Switzerland) 9: PubMed32967340

2020

Paraoxonase 1 Phenotype and Protein N-Homocysteinylation in Patients with Rheumatoid Arthritis: Implications for Cardiovascular Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Jolanta Parada-Turska,Grażyna Wójcicka,Jerzy Beltowski

International journal of environmental research an 17: PubMed32824020

2020

Can Active Aerobic Exercise Reduce the Risk of Cardiovascular Disease in Prehypertensive Elderly Women by Improving HDL Cholesterol and Inflammatory Markers?

Applications

Unspecified application

Species

Unspecified reactive species

Nayoung Ahn,Kijin Kim

Functional & integrative genomics 20:191-200 PubMed31444657

2019

Plasma protein(s)-based conceptual diagnostic tool for assessing high-altitude acclimation in humans.

Applications

Unspecified application

Species

Unspecified reactive species

Subhojit Paul,Anamika Gangwar,Kalpana Bhargava,Nilofar Khan,Pankaj Khurana,Yasmin Ahmad

Molecular oncology 12:1735-1752 PubMed30098223

2018

ABCA1 overexpression worsens colorectal cancer prognosis by facilitating tumour growth and caveolin-1-dependent invasiveness, and these effects can be ameliorated using the BET inhibitor apabetalone.

Applications

Unspecified application

Species

Unspecified reactive species

Cristina Aguirre-Portolés,Jaime Feliu,Guillermo Reglero,Ana Ramírez de Molina

Lipids in health and disease 15:64 PubMed27015844

2016

The mechanism of apoliprotein A1 down-regulated by Hepatitis B virus.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanyuan Wang,Junli Hao,Xiaohong Liu,Hongxin Wang,Xin Zeng,Jing Yang,Lei Li,Xi Kuang,Tao Zhang

Drug design, development and therapy 9:5421-31 PubMed26491253

2015

Decreased expression of the APOA1-APOC3-APOA4 gene cluster is associated with risk of Alzheimer's disease.

Applications

ELISA

Species

Unspecified reactive species

Qiao Lin,Yunpeng Cao,Jie Gao

Bioanalysis 7:2895-911 PubMed26394123

2015

Serum apolipoprotein A-1 quantification by LC-MS with a SILAC internal standard reveals reduced levels in smokers.

Applications

Unspecified application

Species

Unspecified reactive species

Qingqing Wang,Suhong Zhang,Lili Guo,Christine M Busch,Wenying Jian,Naidong Weng,Nathaniel W Snyder,Kannan Rangiah,Clementina Mesaros,Ian A Blair
View all publications
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