Human Apolipoprotein AII ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Apolipoprotein AII with a sensitivity of 6 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Urine, Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 31.25 - 2000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 6 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
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Target data
Function
May stabilize HDL (high density lipoprotein) structure by its association with lipids, and affect the HDL metabolism.
Alternative names
Apolipoprotein A-II, Apo-AII, ApoA-II, Apolipoprotein A2, APOA2
What's included?
Recommended products
Human Apolipoprotein AII ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Apolipoprotein AII with a sensitivity of 6 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Urine, Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 31.25 - 2000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Microplate
- Sensitivity
- = 6 pg/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample HeLa Extract | n 5 | C.V. 2 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample HeLa Extract | n 3 | C.V. 10.6 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Urine | Average % = 90.2 | Range 89.3 - 90.8 % |
Sample type Cell culture media | Average % = 125.2 | Range 122.2 - 127.8 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human Apolipoprotein A II ELISA kit (ab184859) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Apolipoprotein A II protein in human serum, plasma, urine, cell culture supernatant samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate human Apolipoprotein A II with 6 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpeStep ELISA® kits.
Apolipoprotein AII may stabilize HDL (high density lipoprotein) structure by its association with lipids, and affect the HDL metabolism. ApoA2 forms a disulfide-linked homodimer. Apolipoprotein AII also forms a disulfide-linked heterodimer with APOD and interacts with HCV core protein, APOA1BP and NDRG1.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Apolipoprotein A-II (also known as ApoA-II or Apolipoprotein A2) is a pivotal protein component of high-density lipoproteins (HDL) in the plasma. It is encoded by the APOA2 gene in humans and displays a molecular mass of approximately 17.4 kDa. Apolipoprotein A-II is primarily expressed in the liver and secreted into the bloodstream where it plays a direct role in lipid metabolism. Its structural composition includes a highly amphipathic α-helical structure that facilitates interaction with lipids aiding in lipoprotein formation and stability.
- Biological function summary
Apolipoprotein A-II serves as a significant regulator of HDL metabolism and lipid transport. It is an integral part of HDL particles contributing to their stability and function. Apolipoprotein A-II influences lecithin-cholesterol acyltransferase (LCAT) activity which affects cholesterol esterification. Although less studied than its counterpart Apolipoprotein A-I ApoA-II's interaction with lipids and proteins such as CETP (cholesteryl ester transfer protein) suggests its involvement in lipid exchange and reverse cholesterol transport processes.
- Pathways
Apolipoprotein A-II impacts the reverse cholesterol transport pathway and lipid metabolic processes. ApoA-II works closely with its relative Apolipoprotein A-I both part of the same HDL complex. Together they play essential roles in maintaining cholesterol homeostasis and promoting cholesterol efflux from cells back to the liver. The mechanism by which it modulates the function of lipoprotein lipase and hepatic lipase further implicates its role in lipid metabolism and energy balance processes.
- Associated diseases and disorders
Dysfunction or abnormal levels of Apolipoprotein A-II have associations with cardiovascular diseases and metabolic syndromes. Altered ApoA-II levels correlate with an increased risk of atherosclerosis as its imbalance can drive perturbations in HDL composition and function. Moreover research links Apolipoprotein A-II with insulin resistance linking it with ApoA-I imbalanced lipid transport and metabolism in obesity and diabetes contexts. Understanding these connections may guide therapeutic strategies targeting ApoA-II to mitigate the risks and progression of these disorders.
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3 product images
Sandwich ELISA - Human Apolipoprotein AII ELISA Kit (ab184859)
Example of ApoA2 standard curve.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Apolipoprotein AII ELISA Kit (ab184859)
Titration of Human plasma-EDTA within the working range of the assay.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Apolipoprotein AII ELISA Kit (ab184859)
ApoA2 concentrations in 10 individual Human serum donors.
100,000 X diluted serum samples from 10 apparently healthy male donors were measured using this kit. Interpolated data values corrected for sample dilution are graphed in mg of ApoA2 per mL of serum (mean +/- SD, n=3). The mean of ApoA2 concentration of these ten individual serum samples was determined to be 209 μg/mL with a range of 143 – 225 μg/mL.
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