JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB108805

Human Apolipoprotein AII ELISA Kit (APOA2)

1

(1 Review)

|

(1 Publication)

Human Apolipoprotein AII ELISA Kit (APOA2) is a Sandwich (quantitative) ELISA for the measurement of Human Apolipoprotein AII (APOA2) in Human in Biofluids samples.

View Alternative Names

Apolipoprotein A-II, Apo-AII, ApoA-II, Apolipoprotein A2, APOA2

1 Images
Sandwich ELISA - Human Apolipoprotein AII ELISA Kit (APOA2) (AB108805)
  • sELISA

Supplier Data

Sandwich ELISA - Human Apolipoprotein AII ELISA Kit (APOA2) (AB108805)

Representative Standard Curve using ab108805.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.2 µg/mL

Range

0.5 - 32 µg/mL

Assay time

4h

Assay Platform

Microplate

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Abcam's Apolipoprotein AII (APOA2) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of apolipoprotein AII concentrations in plasma, serum, cell culture, cell lysates and tissue samples.

An Apolipoprotein AII specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an Apolipoprotein AII specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Apolipoprotein AII captured in plate.

Get better reproducibility in only 90 minutes with Human Apolipoprotein AII ELISA Kit (ab184859) from our SimpleStep ELISA® range.

The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

style
left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.5" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.6" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab108805-1x96Tests", "assetComponentDetails": [ { "size":"1 x 75 µL", "name":"80X Biotinylated Human Apolipoprotein AII Antibody", "number":"AB108805-CMP01", "productcode":"" }, { "size":"2 x 30 mL", "name":"20X Wash Buffer Concentrate", "number":"AB108805-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Apolipoprotein AII Standard", "number":"AB108805-CMP05", "productcode":"" }, { "size":"1 x 7 mL", "name":"Chromogen Substrate", "number":"AB108805-CMP03", "productcode":"" }, { "size":"3 x 1 Unit", "name":"Sealing Tapes", "number":"AB108805-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Apolipoprotein AII Microplate (12 x 8 well strips)", "number":"AB108805-CMP04", "productcode":"" }, { "size":"1 x 80 µL", "name":"100X Streptavidin-Peroxidase Conjugate", "number":"AB108805-CMP09", "productcode":"" }, { "size":"1 x 30 mL", "name":"10X Diluent M Concentrate", "number":"AB108805-CMP02", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Solution", "number":"AB108805-CMP08", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Apolipoprotein A-II (also known as ApoA-II or Apolipoprotein A2) is a pivotal protein component of high-density lipoproteins (HDL) in the plasma. It is encoded by the APOA2 gene in humans and displays a molecular mass of approximately 17.4 kDa. Apolipoprotein A-II is primarily expressed in the liver and secreted into the bloodstream where it plays a direct role in lipid metabolism. Its structural composition includes a highly amphipathic α-helical structure that facilitates interaction with lipids aiding in lipoprotein formation and stability.
Biological function summary

Apolipoprotein A-II serves as a significant regulator of HDL metabolism and lipid transport. It is an integral part of HDL particles contributing to their stability and function. Apolipoprotein A-II influences lecithin-cholesterol acyltransferase (LCAT) activity which affects cholesterol esterification. Although less studied than its counterpart Apolipoprotein A-I ApoA-II's interaction with lipids and proteins such as CETP (cholesteryl ester transfer protein) suggests its involvement in lipid exchange and reverse cholesterol transport processes.

Pathways

Apolipoprotein A-II impacts the reverse cholesterol transport pathway and lipid metabolic processes. ApoA-II works closely with its relative Apolipoprotein A-I both part of the same HDL complex. Together they play essential roles in maintaining cholesterol homeostasis and promoting cholesterol efflux from cells back to the liver. The mechanism by which it modulates the function of lipoprotein lipase and hepatic lipase further implicates its role in lipid metabolism and energy balance processes.

Dysfunction or abnormal levels of Apolipoprotein A-II have associations with cardiovascular diseases and metabolic syndromes. Altered ApoA-II levels correlate with an increased risk of atherosclerosis as its imbalance can drive perturbations in HDL composition and function. Moreover research links Apolipoprotein A-II with insulin resistance linking it with ApoA-I imbalanced lipid transport and metabolism in obesity and diabetes contexts. Understanding these connections may guide therapeutic strategies targeting ApoA-II to mitigate the risks and progression of these disorders.
style
left-column

Product protocols

style
left-column

Target data

May stabilize HDL (high density lipoprotein) structure by its association with lipids, and affect the HDL metabolism.
See full target information APOA2
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

PloS one 12:e0170741 PubMed28122020

2017

iTRAQ-Based Proteomics Reveals Novel Biomarkers for Idiopathic Pulmonary Fibrosis.

Applications

ELISA

Species

Human

Rui Niu,Ying Liu,Ying Zhang,Yuan Zhang,Hui Wang,Yongbin Wang,Wei Wang,Xiaohui Li
View all publications
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Complementary Products

Assay Kits

AB233623

Human Apo E ELISA Kit

Recombinant|SimpleStep

ELISA - Human Apo E ELISA Kit (AB233623)

  • 0
  • 0
Assay Kits

AB189576

Human Apolipoprotein AI ELISA Kit

SimpleStep

ELISA - Human Apolipoprotein AI ELISA Kit (AB189576)

  • 0
  • 0
Assay Kits

AB190806

Human Apolipoprotein B ELISA Kit

Recombinant|SimpleStep

ELISA - Human Apolipoprotein B ELISA Kit (AB190806)

  • 4
  • 1
Assay Kits

AB214567

Human Apolipoprotein A-IV ELISA Kit

Recombinant|SimpleStep

ELISA - Human Apolipoprotein A-IV ELISA Kit (AB214567)

  • 4
  • 1

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com